FastQCFastQC Report
Thu 26 May 2016
SRR1546551_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546551_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1894795
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112460.5935206711016231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG502.7284841E-1144.01
GTACGTT207.8577764E-444.09
CGTTTTT94450.042.485971
CGCGTAA404.126432E-738.531
CGTATGG1550.038.322582
GACCGAT5250.037.7142839
ATATACG1000.037.3999981
GCGTAAG651.0913936E-1137.2307661
TACGGGA4550.036.7472534
GGCACCG5000.036.528
TTTAGCG1750.036.457141
TAATGCG1150.036.3478281
TAGGGAT23000.036.252175
ACGGGAT6100.036.0655755
CATACGA3850.036.018
GCGTAAC553.426976E-936.032
AAATGCG1100.036.01
CGGTCTA1350.035.85185231
ACGGGAC3750.035.7866675
GTAGGGA24000.035.4754
Produced by FastQC (version 0.11.5)