##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546551_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1894795
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.741098113516237	31.0	31.0	31.0	28.0	34.0
2	31.072532912531436	31.0	31.0	33.0	28.0	34.0
3	31.209067471678996	31.0	31.0	34.0	28.0	34.0
4	35.04421058742503	35.0	35.0	37.0	32.0	37.0
5	34.86387181726783	35.0	35.0	37.0	32.0	37.0
6	34.89607371773727	35.0	35.0	37.0	32.0	37.0
7	35.491198256275744	36.0	35.0	37.0	35.0	37.0
8	34.45127889824493	37.0	35.0	37.0	33.0	37.0
9	36.60628722368383	39.0	37.0	39.0	32.0	39.0
10	36.09880857823669	38.0	35.0	39.0	31.0	39.0
11	35.80239603756607	37.0	35.0	39.0	30.0	39.0
12	35.293119308421225	37.0	34.0	39.0	30.0	39.0
13	34.99612095239854	37.0	34.0	39.0	27.0	39.0
14	35.92474383772387	38.0	34.0	40.0	28.0	41.0
15	36.22765417894812	38.0	34.0	40.0	30.0	41.0
16	36.34913750564045	38.0	34.0	40.0	30.0	41.0
17	36.26625360527128	38.0	34.0	40.0	30.0	41.0
18	36.16639425373194	38.0	34.0	40.0	30.0	41.0
19	36.13704173802443	37.0	34.0	40.0	30.0	41.0
20	35.96131718734745	37.0	34.0	40.0	30.0	41.0
21	35.76386891457915	37.0	34.0	40.0	29.0	41.0
22	35.70111436857285	37.0	34.0	40.0	29.0	41.0
23	35.64897416343193	37.0	34.0	40.0	29.0	41.0
24	35.569534435123586	37.0	34.0	40.0	29.0	41.0
25	35.42508661886906	37.0	34.0	40.0	29.0	41.0
26	35.24439847054695	37.0	34.0	40.0	27.0	41.0
27	35.07026987088313	37.0	33.0	40.0	27.0	41.0
28	35.07139928066097	36.0	34.0	40.0	27.0	41.0
29	34.962677756696635	36.0	33.0	40.0	27.0	41.0
30	34.967845598072614	36.0	33.0	40.0	27.0	41.0
31	34.87367287754084	37.0	33.0	40.0	26.0	41.0
32	34.60148248227381	37.0	33.0	40.0	25.0	41.0
33	34.40730791457651	37.0	33.0	40.0	24.0	41.0
34	34.249000551510846	36.0	33.0	40.0	24.0	41.0
35	34.04065294662483	36.0	33.0	40.0	23.0	41.0
36	33.986564245736346	36.0	33.0	40.0	23.0	41.0
37	33.992939077842195	36.0	33.0	40.0	23.0	41.0
38	34.03603397729042	36.0	33.0	40.0	23.0	41.0
39	33.963742251800326	36.0	33.0	40.0	23.0	41.0
40	33.88848028414684	36.0	33.0	40.0	23.0	41.0
41	33.91418280077792	36.0	33.0	40.0	23.0	41.0
42	33.77672202006022	36.0	32.0	40.0	23.0	41.0
43	33.81087927717774	36.0	33.0	40.0	23.0	41.0
44	33.534395013708604	36.0	32.0	40.0	22.0	41.0
45	33.451195511915536	35.0	32.0	40.0	22.0	41.0
46	33.40298924158022	35.0	32.0	40.0	22.0	41.0
47	33.36552714145857	35.0	32.0	40.0	22.0	41.0
48	33.38932232774522	35.0	32.0	39.0	22.0	41.0
49	33.40584390395795	35.0	32.0	39.0	22.0	41.0
50	32.84018271105845	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	4.0
12	6.0
13	7.0
14	37.0
15	74.0
16	246.0
17	604.0
18	1325.0
19	2554.0
20	4440.0
21	7207.0
22	10672.0
23	15504.0
24	21805.0
25	29410.0
26	38792.0
27	46411.0
28	51647.0
29	58310.0
30	68178.0
31	83279.0
32	101680.0
33	126659.0
34	178684.0
35	199761.0
36	163590.0
37	197185.0
38	239102.0
39	247620.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.6355647972472	23.247369768233504	27.647951361492932	15.469114073026368
2	33.92630865080391	24.80115263128729	28.064196918400143	13.208341799508656
3	28.986618605178926	24.920426748012318	32.81763990299742	13.275314743811336
4	26.27619346683942	27.574803606722625	31.458601062384055	14.690401864053895
5	23.849176296116468	30.474220166297673	30.77182492037397	14.904778617211889
6	20.887378317971073	40.84790175190456	27.94012017131141	10.32459975881296
7	87.04931140308054	3.3844294501515995	8.154919133732145	1.411340013035711
8	85.30680099958043	5.7737116680168565	7.595597412912743	1.3238899194899714
9	83.33946416366942	4.8900804572526315	9.080507389981502	2.6899479890964457
10	44.024604244786374	30.226911090645693	14.995078623281147	10.753406041286787
11	35.254473439079156	24.749801429706118	26.073480244564717	13.922244886650006
12	33.078301346583665	23.04201773806665	28.17909061402421	15.700590301325473
13	22.33339226670959	32.00773698473978	28.14890265173805	17.509968096812585
14	17.255587016009645	34.14897126074325	30.933161634899818	17.662280088347288
15	17.405101871178676	25.194862768795566	40.842624136120264	16.55741122390549
16	20.284885700036153	22.94659844468663	39.175953071440446	17.592562783836772
17	20.3573473647545	22.183507978435664	30.54631239791112	26.91283225889872
18	22.573840441841995	24.48154021939049	33.4506899163234	19.49392942244412
19	26.987035536825882	25.671853683379997	27.581083969505936	19.760026810288185
20	29.2989479072934	24.789964085824586	27.65022073628018	18.260867270601832
21	24.401953773363346	27.18515723336825	29.691496969329133	18.721392023939266
22	23.20535994659053	24.23032570805813	29.592225016426575	22.972089328924767
23	21.85027931781538	28.39826999754591	28.75487849609061	20.9965721885481
24	20.951395797434554	24.767270337952127	35.44436205499804	18.83697180961529
25	20.033723964861633	26.23545027298468	33.410949469467674	20.319876292686015
26	19.151095501096425	31.656511654295056	29.422813549750764	19.769579294857756
27	19.69474270303648	30.035016980728784	30.610171548901064	19.66006876733367
28	17.831374898076042	28.448565675970222	35.718164761887174	18.001894664066562
29	19.785939903789064	25.77128396475608	34.723967500442	19.718808631012855
30	20.85814032652609	27.701255280914296	31.621784942434406	19.81881945012521
31	26.50492533493069	26.025770597874704	27.573167545829495	19.896136521365108
32	26.981599592568063	26.593853160896035	28.148374890159623	18.276172356376282
33	26.225158922205306	26.895680007599765	27.585200509817685	19.293960560377243
34	20.749790874474545	27.3476022472088	30.065099390699256	21.837507487617394
35	21.220131993170767	27.036909005987454	30.814256951279688	20.92870204956209
36	25.822265733232353	27.742315131716094	27.50788343857779	18.92753569647376
37	22.160128140511244	30.99739021899467	27.66146205790072	19.18101958259337
38	21.275177525800945	31.558822986127787	26.372351626429246	20.793647861642025
39	21.942057056304243	29.9870962294074	27.198667929776043	20.872178784512307
40	23.481537580582597	26.52339699017572	27.35298541530878	22.642080013932905
41	19.29184951406353	26.926659612253566	28.468251182845638	25.313239690837268
42	21.916882829013165	27.5948057705451	26.08662150786761	24.40168989257413
43	21.891708601722083	27.184418367158454	26.885810866083137	24.038062165036322
44	20.868167796516246	29.483083922007392	28.749125894885726	20.899622386590632
45	20.379671679522062	31.891101675906892	26.243525025134645	21.485701619436405
46	21.492562519955985	31.11640045493048	27.009201523119913	20.38183550199362
47	21.43255602848857	28.011684641346424	28.154180267522342	22.40157906264266
48	22.20456566541499	25.5635570074863	30.897854385302896	21.334022941795812
49	21.251322702455937	26.27439907747276	30.706382484648735	21.767895735422567
50	19.83412453589966	30.731187278834916	28.091746072794155	21.34294211247127
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	487.0
1	1438.5
2	2390.0
3	12311.5
4	22233.0
5	16767.0
6	11301.0
7	11238.5
8	11176.0
9	11237.0
10	11298.0
11	11337.5
12	11377.0
13	10982.5
14	10588.0
15	10060.0
16	9532.0
17	9001.0
18	8470.0
19	8321.0
20	8172.0
21	8435.0
22	8698.0
23	10536.0
24	12374.0
25	13592.0
26	14810.0
27	18859.5
28	22909.0
29	27365.5
30	31822.0
31	37485.5
32	43149.0
33	48309.5
34	53470.0
35	60357.0
36	67244.0
37	71147.0
38	75050.0
39	88956.0
40	102862.0
41	116197.5
42	129533.0
43	144041.0
44	158549.0
45	157239.5
46	155930.0
47	151101.0
48	146272.0
49	140198.0
50	134124.0
51	127259.0
52	120394.0
53	113800.0
54	107206.0
55	97350.5
56	87495.0
57	82429.0
58	77363.0
59	73789.0
60	70215.0
61	64466.5
62	58718.0
63	49947.0
64	41176.0
65	33030.0
66	24884.0
67	20657.5
68	16431.0
69	13727.0
70	11023.0
71	9033.5
72	7044.0
73	5502.5
74	3961.0
75	3273.5
76	2586.0
77	1932.0
78	1278.0
79	995.0
80	712.0
81	525.0
82	338.0
83	211.5
84	85.0
85	61.5
86	38.0
87	26.0
88	14.0
89	10.0
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1894795.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.66224850063098
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.42687721465079	18.320940092809096
2	5.9110886436741295	2.7973929679175
3	2.2694137107542875	1.6109829352382108
4	1.369852162035289	1.2965512906882253
5	1.0215816274720908	1.208645916646182
6	0.7943595437556846	1.1277799753916917
7	0.6813376536891016	1.1285386612099861
8	0.5801909247296657	1.0982897471011388
9	0.5119481950324839	1.0902460869277317
>10	8.008011313181859	45.838426582585235
>50	1.3276785573239307	20.41114248870312
>100	0.0960852860273425	3.2471388747967387
>500	0.0011251197666949982	0.16394359351003043
>1k	2.2502395333899965E-4	0.06117934035174569
>5k	0.0	0.0
>10k+	2.2502395333899965E-4	0.5988014461233525
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11246	0.5935206711016231	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.006491467414680744	0.0
2	0.0	0.0	0.0	0.025965869658722975	0.0
3	0.0	0.0	0.0	0.04475418185080708	0.0
4	0.0	0.0	0.0	0.06443968872622104	0.0
5	0.0	0.0	0.0	0.10845500436722706	0.0
6	0.0	0.0	0.0	0.17822508503558432	0.0
7	0.0	0.0	0.0	0.22904852503832868	0.0
8	0.0	0.0	0.0	0.372652450528949	0.0
9	0.0	0.0	0.0	0.4617386049678197	0.0
10	0.0	0.0	0.0	0.589668011579089	0.0
11	0.0	0.0	0.0	0.6795458083856037	0.0
12	0.0	0.0	0.0	0.7648320794597833	0.0
13	0.0	0.0	0.0	0.8091640520478469	0.0
14	0.0	0.0	0.0	0.8340216223918683	0.0
15	0.0	0.0	0.0	0.8559765040545283	0.0
16	0.0	0.0	0.0	0.8918115152298798	0.0
17	0.0	0.0	0.0	0.9342435461355978	0.0
18	0.0	0.0	0.0	1.0058607923284577	0.0
19	0.0	0.0	0.0	1.0452318060792856	0.0
20	0.0	0.0	0.0	1.0859749999340298	0.0
21	0.0	0.0	0.0	1.1332624373612976	0.0
22	0.0	0.0	0.0	1.1806554271042513	0.0
23	0.0	0.0	0.0	1.2359120643658021	0.0
24	0.0	0.0	0.0	1.279135737639164	0.0
25	0.0	0.0	0.0	1.3187178560213637	0.0
26	0.0	0.0	0.0	1.357033346615333	0.0
27	5.2776157842932874E-5	0.0	0.0	1.4005209006779098	0.0
28	5.2776157842932874E-5	0.0	0.0	1.4448528732659733	0.0
29	5.2776157842932874E-5	0.0	0.0	1.4911375636942255	0.0
30	5.2776157842932874E-5	0.0	0.0	1.559271583469452	0.0
31	5.2776157842932874E-5	0.0	0.0	1.6193836272525524	0.0
32	5.2776157842932874E-5	0.0	0.0	1.681131731928784	0.0
33	5.2776157842932874E-5	0.0	0.0	1.7392910578716958	0.0
34	5.2776157842932874E-5	0.0	0.0	1.795022680553833	0.0
35	5.2776157842932874E-5	0.0	0.0	1.8666399267466929	0.0
36	5.2776157842932874E-5	0.0	0.0	1.9303935254209559	0.0
37	5.2776157842932874E-5	0.0	0.0	1.9988442021432398	0.0
38	5.2776157842932874E-5	0.0	0.0	2.0723086138606024	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	50	2.7284841E-11	44.0	1
GTACGTT	20	7.8577764E-4	44.0	9
CGTTTTT	9445	0.0	42.48597	1
CGCGTAA	40	4.126432E-7	38.5	31
CGTATGG	155	0.0	38.32258	2
GACCGAT	525	0.0	37.714283	9
ATATACG	100	0.0	37.399998	1
GCGTAAG	65	1.0913936E-11	37.230766	1
TACGGGA	455	0.0	36.747253	4
GGCACCG	500	0.0	36.52	8
TTTAGCG	175	0.0	36.45714	1
TAATGCG	115	0.0	36.347828	1
TAGGGAT	2300	0.0	36.25217	5
ACGGGAT	610	0.0	36.065575	5
CATACGA	385	0.0	36.0	18
GCGTAAC	55	3.426976E-9	36.0	32
AAATGCG	110	0.0	36.0	1
CGGTCTA	135	0.0	35.851852	31
ACGGGAC	375	0.0	35.786667	5
GTAGGGA	2400	0.0	35.475	4
>>END_MODULE