FastQCFastQC Report
Thu 26 May 2016
SRR1546550_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546550_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2133432
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156630.7341691696759025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT125450.043.053011
CGTTAGG2400.040.3333362
GCGTAAG1700.040.117651
CGATTAC404.1267594E-738.510
ACGGGAT8150.038.061355
GCGATGT2200.038.09
GGGCGAT23500.037.165967
GCGTTAG1600.037.1251
CGACCCG2550.037.0980432
AGGGATC20150.036.6848646
GTTTTTT147200.036.646742
CGGGATC5000.036.526
TGCGGGA11200.036.1428574
GTAGGGC9500.036.1263164
TAGGGAT24350.036.0492825
GCGGGAT9350.036.05
TGGGCGA8400.035.8809556
CGTAAGG3250.035.8769232
CGGGATT8600.035.8139536
AGGGCGA13050.035.7394646
Produced by FastQC (version 0.11.5)