##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546550_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2133432
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.64499079417577	31.0	30.0	31.0	28.0	34.0
2	30.970173410729753	31.0	31.0	33.0	28.0	34.0
3	31.1097672670139	31.0	31.0	34.0	28.0	34.0
4	34.93986965602841	35.0	35.0	37.0	32.0	37.0
5	34.74132993224063	35.0	35.0	37.0	32.0	37.0
6	34.778134480030296	35.0	35.0	37.0	32.0	37.0
7	35.43331917773803	35.0	35.0	37.0	35.0	37.0
8	34.39955573929706	36.0	35.0	37.0	33.0	37.0
9	36.526198163334946	39.0	37.0	39.0	32.0	39.0
10	35.96012856280397	38.0	35.0	39.0	30.0	39.0
11	35.62845593391306	37.0	35.0	39.0	30.0	39.0
12	35.1700804150308	37.0	34.0	39.0	30.0	39.0
13	34.96685200184491	37.0	34.0	39.0	27.0	39.0
14	35.857445186910105	38.0	34.0	40.0	27.0	41.0
15	36.09489451737857	38.0	34.0	40.0	30.0	41.0
16	36.19230094983107	38.0	34.0	40.0	30.0	41.0
17	36.08465092864455	38.0	34.0	40.0	30.0	41.0
18	35.97906799935503	38.0	34.0	40.0	29.0	41.0
19	35.961762081003755	37.0	34.0	40.0	29.0	41.0
20	35.7846502724249	37.0	34.0	40.0	29.0	41.0
21	35.621071587939056	37.0	34.0	40.0	29.0	41.0
22	35.585888840141145	37.0	34.0	40.0	29.0	41.0
23	35.51964112284807	37.0	34.0	40.0	29.0	41.0
24	35.45284030613584	37.0	34.0	40.0	29.0	41.0
25	35.26402528883039	37.0	34.0	40.0	27.0	41.0
26	35.09455281443233	37.0	33.0	40.0	27.0	41.0
27	34.89762082878667	36.0	33.0	40.0	27.0	41.0
28	34.903351501243066	36.0	33.0	40.0	27.0	41.0
29	34.75585113563498	36.0	33.0	40.0	26.0	41.0
30	34.74731043689229	36.0	33.0	40.0	26.0	41.0
31	34.636090112082314	36.0	33.0	40.0	25.0	41.0
32	34.35677537413895	36.0	33.0	40.0	24.0	41.0
33	34.159182012831906	36.0	33.0	40.0	23.0	41.0
34	33.98418276279722	36.0	33.0	40.0	23.0	41.0
35	33.76946769336918	36.0	32.0	40.0	23.0	41.0
36	33.76005937850375	36.0	32.0	40.0	22.0	41.0
37	33.794075461509905	36.0	32.0	40.0	23.0	41.0
38	33.81630958943149	36.0	32.0	40.0	23.0	41.0
39	33.72927658345802	36.0	32.0	40.0	22.0	41.0
40	33.66113098519193	36.0	32.0	40.0	22.0	41.0
41	33.69321825115588	36.0	32.0	40.0	22.0	41.0
42	33.54051218881127	36.0	32.0	40.0	21.0	41.0
43	33.593790662181874	36.0	32.0	40.0	22.0	41.0
44	33.30799013045647	36.0	32.0	40.0	21.0	41.0
45	33.23657468342089	35.0	31.0	40.0	20.0	41.0
46	33.17892063117081	35.0	31.0	39.0	20.0	41.0
47	33.13458080688768	35.0	31.0	39.0	20.0	41.0
48	33.145802631628285	35.0	31.0	39.0	20.0	41.0
49	33.15925232208011	35.0	31.0	39.0	20.0	41.0
50	32.61716801847915	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	2.0
12	2.0
13	11.0
14	40.0
15	116.0
16	327.0
17	832.0
18	1820.0
19	3645.0
20	5917.0
21	9112.0
22	13638.0
23	19146.0
24	26587.0
25	36525.0
26	47124.0
27	55697.0
28	62368.0
29	70141.0
30	81787.0
31	97076.0
32	116648.0
33	142667.0
34	196529.0
35	210477.0
36	184736.0
37	218567.0
38	264227.0
39	267663.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.84955883290398	23.76424465368477	28.24125634189419	15.144940171517069
2	34.11868763569685	24.60247151069263	28.328861665147986	12.949979188462535
3	29.110044285451796	25.035810843748475	32.9306488324915	12.923496038308228
4	26.427699593893784	27.212632040768113	32.148013154391606	14.211655210946494
5	23.35410737253402	30.91605450747903	31.14245028667424	14.58738783331271
6	20.76396154177869	40.67638434222417	28.688329414764567	9.871324701232568
7	86.44967357759704	3.4795578204508044	8.64531890400069	1.4254496979514697
8	85.21166833533948	5.32442562031506	8.032925352202462	1.4309806921429884
9	83.06189276245973	4.694782866292433	9.632882604179557	2.610441767068273
10	44.28390499439401	29.344736555934286	15.54443731977396	10.826921129897741
11	34.191902999486274	24.483180152917928	26.90238076488962	14.422536082706175
12	31.09417126957878	22.803398467820863	29.608114999681263	16.49431526291909
13	23.478976597332373	27.90044397946595	30.106560696567787	18.514018726633893
14	18.239390803175354	30.51819790834674	32.556275522257096	18.68613576622081
15	18.47886410253526	24.8238987696819	39.858875277018434	16.838361850764404
16	22.762103502713	22.81019502847993	37.00244488692398	17.42525658188309
17	22.153647268813817	22.576487087472206	31.118638887951434	24.151226755762544
18	23.276907818013417	24.63561060300961	33.33398955298318	18.753492025993797
19	26.657095234345412	25.872256533135342	28.75774807915134	18.712900153367904
20	28.314612324180004	25.45705698611439	28.370859722737823	17.857470966967778
21	25.015561780267664	26.512867529876743	30.205696736525933	18.26587395332966
22	23.423104181431608	23.63492250983392	31.150840523625785	21.791132785108687
23	21.928376437589762	26.49543083632382	30.911882825419323	20.664309900667092
24	22.187301962284245	23.84538152610442	34.453969003933565	19.51334750767777
25	21.54326924879724	25.359280258288052	32.479966551546994	20.617483941367713
26	20.05201009453313	29.171635186872606	29.931068813067398	20.845285905526868
27	19.701729420014324	28.29000408731096	31.934038675711246	20.074227816963468
28	18.65009055831168	27.45435523607033	34.57063548310891	19.324918722509086
29	20.802116027133746	26.004250428417684	33.45923376043858	19.73439978400999
30	21.854411108486232	26.532460373707718	31.859463999790012	19.75366451801604
31	26.427465229733127	25.106635693099193	28.985175060653447	19.480724016514237
32	27.355453560272835	25.836445689386867	28.697938345351524	18.11016240498877
33	26.24962970462616	26.128791543391117	28.377328173571968	19.24425057841075
34	21.778289629104655	26.89759973601221	30.355502308018256	20.96860832686488
35	21.380058047315313	26.821243892469976	31.074250315922892	20.724447744291826
36	25.304064062037128	27.877991892874952	27.661251917098834	19.156692127989082
37	22.928361438283478	30.16829221648499	27.401623299922377	19.501723045309156
38	22.22034730893696	30.779092091990744	25.978329752248957	21.022230846823334
39	22.83400642720274	29.318628388437034	27.072997873848337	20.774367310511888
40	23.662671226455775	26.007812763659683	27.562069004308547	22.767447005575992
41	20.37163593683792	26.570286749237848	28.181071625437326	24.877005688486907
42	21.480740890733806	27.241036977039812	26.766637043036763	24.511585089189623
43	21.23358044690433	26.42873079620068	27.862195748446634	24.47549300844836
44	20.856910367895484	27.811151234255416	29.0877328173572	22.2442055804919
45	21.230346221487256	29.641254091998242	27.229084404846276	21.899315281668223
46	21.771868051102636	29.741655698423948	27.42023181427859	21.066244436194825
47	21.33201339438051	27.37439018445397	29.01461119923204	22.278985221933485
48	21.782461311164358	25.590878921849864	30.992316605357	21.634343161628774
49	21.984014489329866	25.473556223024683	30.633880058047314	21.908549229598133
50	20.370557861698895	28.833588321540127	29.051500118119538	21.74435369864144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	550.0
1	1690.0
2	2830.0
3	16346.5
4	29863.0
5	21837.0
6	13811.0
7	13699.5
8	13588.0
9	13643.0
10	13698.0
11	13463.0
12	13228.0
13	12896.5
14	12565.0
15	11800.5
16	11036.0
17	10503.0
18	9970.0
19	9883.0
20	9796.0
21	10503.0
22	11210.0
23	11884.5
24	12559.0
25	15445.5
26	18332.0
27	21438.5
28	24545.0
29	28839.0
30	33133.0
31	39513.0
32	45893.0
33	51822.5
34	57752.0
35	63908.0
36	70064.0
37	77072.0
38	84080.0
39	97269.0
40	110458.0
41	124292.0
42	138126.0
43	152109.0
44	166092.0
45	165920.0
46	165748.0
47	160915.5
48	156083.0
49	150457.5
50	144832.0
51	138360.0
52	131888.0
53	123121.0
54	114354.0
55	109325.0
56	104296.0
57	101990.0
58	99684.0
59	96501.0
60	93318.0
61	86870.0
62	80422.0
63	67765.5
64	55109.0
65	44739.0
66	34369.0
67	27411.5
68	20454.0
69	16468.5
70	12483.0
71	10145.5
72	7808.0
73	6057.5
74	4307.0
75	3396.0
76	2485.0
77	1882.5
78	1280.0
79	1039.5
80	799.0
81	556.5
82	314.0
83	224.0
84	134.0
85	89.5
86	45.0
87	32.5
88	20.0
89	13.5
90	7.0
91	8.5
92	10.0
93	6.0
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2133432.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.564008270342864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68878557388027	19.32911979620031
2	5.161901793236634	2.5359399667952465
3	2.0052875333682674	1.4777369866222068
4	1.2631082205630881	1.2410800310499899
5	0.9851517584131189	1.2099637970601331
6	0.8328899629973623	1.227546956361166
7	0.66502601659057	1.1434993200066752
8	0.5900935981384773	1.1596051219959946
9	0.5180019716021369	1.145178424303992
>10	8.01658067487804	46.63076992925043
>50	1.1918263766697959	19.105013842336525
>100	0.08017607673475767	2.872077380574761
>500	9.753691062281992E-4	0.1719296031075815
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	1.9507382124563983E-4	0.7505388443350194
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15663	0.7341691696759025	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00932769359417127	0.0
2	0.0	0.0	0.0	0.03693579171963297	0.0
3	0.0	0.0	0.0	0.06309083204901773	0.0
4	0.0	0.0	0.0	0.09487061223418417	0.0
5	0.0	0.0	0.0	0.17099209161576276	0.0
6	0.0	0.0	0.0	0.28747107946257483	0.0
7	0.0	0.0	0.0	0.37095159348880113	0.0
8	0.0	0.0	0.0	0.5742859392753085	0.0
9	0.0	0.0	0.0	0.6971396322920065	0.0
10	0.0	0.0	0.0	0.8561791517142332	0.0
11	0.0	0.0	0.0	0.9606118217032462	0.0
12	0.0	0.0	0.0	1.0545918501269316	0.0
13	0.0	0.0	0.0	1.104417670682731	0.0
14	0.0	0.0	0.0	1.126307283288148	0.0
15	0.0	0.0	0.0	1.1511967571499817	0.0
16	0.0	0.0	0.0	1.199382028581178	0.0
17	0.0	0.0	0.0	1.2535670225252082	0.0
18	0.0	0.0	0.0	1.333625819805834	0.0
19	0.0	0.0	0.0	1.3768894438632213	0.0
20	0.0	0.0	0.0	1.4241372586517873	0.0
21	0.0	0.0	0.0	1.4842751022765197	0.0
22	0.0	0.0	0.0	1.545866003697329	0.0
23	0.0	0.0	0.0	1.6128941536453938	0.0
24	0.0	0.0	0.0	1.667641621575002	0.0
25	0.0	0.0	0.0	1.7162956213275136	0.0
26	0.0	0.0	0.0	1.7649027482478936	0.0
27	0.0	0.0	0.0	1.8167909734174794	0.0
28	0.0	0.0	0.0	1.8730852448074276	0.0
29	0.0	0.0	0.0	1.9391759381128624	0.0
30	0.0	0.0	0.0	2.0265000243738727	0.0
31	0.0	0.0	0.0	2.0997153881632973	0.0
32	0.0	0.0	0.0	2.174571301077325	0.0
33	0.0	0.0	0.0	2.249802196648405	0.0
34	0.0	0.0	0.0	2.3305640864110035	0.0
35	0.0	0.0	0.0	2.4184975194897236	0.0
36	0.0	0.0	0.0	2.5031967271513693	0.0
37	0.0	0.0	0.0	2.5944112584792953	0.0
38	0.0	0.0	0.0	2.700625096089306	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	12545	0.0	43.05301	1
CGTTAGG	240	0.0	40.333336	2
GCGTAAG	170	0.0	40.11765	1
CGATTAC	40	4.1267594E-7	38.5	10
ACGGGAT	815	0.0	38.06135	5
GCGATGT	220	0.0	38.0	9
GGGCGAT	2350	0.0	37.16596	7
GCGTTAG	160	0.0	37.125	1
CGACCCG	255	0.0	37.09804	32
AGGGATC	2015	0.0	36.684864	6
GTTTTTT	14720	0.0	36.64674	2
CGGGATC	500	0.0	36.52	6
TGCGGGA	1120	0.0	36.142857	4
GTAGGGC	950	0.0	36.126316	4
TAGGGAT	2435	0.0	36.049282	5
GCGGGAT	935	0.0	36.0	5
TGGGCGA	840	0.0	35.880955	6
CGTAAGG	325	0.0	35.876923	2
CGGGATT	860	0.0	35.813953	6
AGGGCGA	1305	0.0	35.739464	6
>>END_MODULE