FastQCFastQC Report
Thu 26 May 2016
SRR1546549_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546549_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2055156
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45390.22085914645895494No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25240.12281306139290643No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCG207.857931E-444.0000041
TTTCGCG254.444068E-544.01
ATATCGT254.444068E-544.026
CGCGTAA351.4468242E-744.031
GCTAACG254.444068E-544.01
CGATCGA254.444068E-544.041
GCGTATC254.444068E-544.027
CGTTTTT45200.041.1283151
ACGGGTA1400.040.8571435
CGGTCTA1650.040.031
CGGGTAT2450.039.5102046
ACGGGAT7700.039.428575
CGTATCT452.352499E-839.1111128
ACCGGGT850.038.8235324
TGCGTAG1700.038.8235321
TATGGGC4450.038.56184
TGCGAAC404.1266503E-738.50000411
AACGGGT1200.038.54
GTAGTAG4500.038.1333351
TCGATAG750.038.133331
Produced by FastQC (version 0.11.5)