##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546549_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2055156
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.626175823149193	31.0	30.0	31.0	28.0	33.0
2	30.93713421268264	31.0	31.0	33.0	28.0	34.0
3	31.120168006710927	31.0	31.0	34.0	28.0	34.0
4	34.92699386323958	35.0	35.0	37.0	32.0	37.0
5	34.70494843213849	35.0	35.0	37.0	32.0	37.0
6	34.75255747008986	35.0	35.0	37.0	32.0	37.0
7	35.42046637822141	35.0	35.0	37.0	35.0	37.0
8	34.39879454406381	35.0	35.0	37.0	33.0	37.0
9	36.55265488361954	39.0	37.0	39.0	32.0	39.0
10	35.97477223140238	38.0	35.0	39.0	30.0	39.0
11	35.54508222246876	37.0	35.0	39.0	30.0	39.0
12	34.597673363968475	35.0	33.0	39.0	27.0	39.0
13	33.97921033731746	35.0	33.0	39.0	24.0	39.0
14	34.847848533152714	37.0	33.0	40.0	25.0	41.0
15	35.3920398256872	37.0	33.0	40.0	27.0	41.0
16	35.64702582188408	37.0	34.0	40.0	29.0	41.0
17	35.612791924311345	37.0	34.0	40.0	29.0	41.0
18	35.55227243090062	36.0	34.0	40.0	29.0	41.0
19	35.5594699380485	36.0	34.0	40.0	29.0	41.0
20	35.38134817989486	36.0	34.0	40.0	29.0	41.0
21	35.1473605896584	36.0	33.0	40.0	28.0	41.0
22	35.10369723758196	36.0	33.0	40.0	27.0	41.0
23	35.10416192250126	36.0	33.0	40.0	29.0	41.0
24	35.0473107637571	36.0	33.0	40.0	28.0	41.0
25	34.901363205518216	35.0	33.0	40.0	27.0	41.0
26	34.70479661884548	35.0	33.0	40.0	27.0	41.0
27	34.496291279104845	35.0	33.0	39.0	26.0	41.0
28	34.58614333899714	36.0	33.0	39.0	26.0	41.0
29	34.54870335877179	36.0	33.0	39.0	26.0	41.0
30	34.594723222957285	36.0	33.0	39.0	26.0	41.0
31	34.4885006296359	35.0	33.0	39.0	26.0	41.0
32	34.26131446955851	35.0	33.0	39.0	25.0	41.0
33	34.125551539639815	35.0	33.0	39.0	24.0	41.0
34	34.10898491404059	35.0	33.0	39.0	24.0	41.0
35	33.922364044383976	35.0	32.0	39.0	24.0	41.0
36	33.83275430186322	35.0	32.0	39.0	23.0	41.0
37	33.84604769662254	35.0	32.0	40.0	23.0	41.0
38	33.938467931388175	35.0	32.0	40.0	24.0	41.0
39	33.90913633806874	35.0	32.0	40.0	23.0	41.0
40	33.79754967506116	35.0	32.0	39.0	23.0	41.0
41	33.89433259567644	35.0	33.0	39.0	23.0	41.0
42	33.74169308801862	35.0	32.0	39.0	23.0	41.0
43	33.80061708210958	35.0	32.0	39.0	23.0	41.0
44	33.52449546409129	35.0	32.0	39.0	23.0	41.0
45	33.45420542284868	35.0	32.0	39.0	23.0	41.0
46	33.4240539404308	35.0	32.0	39.0	23.0	41.0
47	33.37830656164301	35.0	32.0	39.0	23.0	41.0
48	33.456083139187484	35.0	32.0	39.0	23.0	41.0
49	33.503546689399734	35.0	32.0	39.0	24.0	41.0
50	32.872894807012216	35.0	31.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	8.0
13	9.0
14	26.0
15	104.0
16	262.0
17	728.0
18	1525.0
19	3100.0
20	5246.0
21	8333.0
22	12360.0
23	17530.0
24	24057.0
25	31985.0
26	40973.0
27	49984.0
28	59262.0
29	69340.0
30	83119.0
31	101036.0
32	122178.0
33	152406.0
34	225069.0
35	246068.0
36	157853.0
37	187155.0
38	225535.0
39	229902.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.24077977535525	23.6487157179309	29.104895200169718	12.005609306544127
2	30.354921962128422	25.60287394241605	30.75104760903795	13.291156486417577
3	29.223718296810556	26.197135399940443	31.22930814011199	13.349838163137006
4	26.2582499819965	28.153483239228557	30.601180640301756	14.987086138473185
5	22.90731214564734	32.31234027976465	29.75010169544307	15.03024587914494
6	21.080978767548547	41.61708405590622	26.940047373532718	10.36188980301252
7	90.01725416464735	3.6609386343421133	4.940695499514392	1.3811117014961394
8	88.97324582659418	5.34718532315795	4.27106263466131	1.408506215586554
9	88.07501717631168	4.0881081533469965	5.661711325077025	2.175163345264301
10	53.69748087249825	25.969658750965863	10.756409732399876	9.576450644136017
11	46.353804771997844	21.169974444762342	20.629042272216804	11.847178511023007
12	41.953311573428	22.716669683469284	21.390590300687638	13.939428442415078
13	22.23680343487307	39.65134520201873	22.385940532008274	15.725910831099926
14	15.746201261607393	40.74663918456798	27.067774903705605	16.439384650119017
15	15.260836646950402	23.572225174147363	46.36776964863008	14.799168530272155
16	18.16290344869197	19.814310933087317	43.89759220224645	18.125193415974262
17	18.477575424931246	20.04013320643299	27.320651084394566	34.161640284241194
18	22.634729431731703	24.254119881897044	33.173588768930436	19.937561917440817
19	30.34519034078192	24.963311787523672	24.386859197063384	20.304638674631025
20	32.50595088645339	24.699390216606428	23.94523822035894	18.849420676581243
21	23.721508245602767	28.914982609592656	27.032546434431254	20.330962710373324
22	22.77432953994733	25.112740833299274	26.052718139158294	26.060211487595105
23	20.78377505162625	30.43778671789392	25.823733088875006	22.954705141604823
24	20.775843780228847	23.558746878582454	37.40820648164908	18.257202859539618
25	18.75263970229024	25.484099503881946	34.51616324989441	21.247097543933403
26	18.04155013050104	35.27352668118625	26.251973086228002	20.432950102084707
27	18.662573546728325	33.54981325018636	28.50260515503446	19.28500804805085
28	16.086272769561045	28.702492657491696	37.48907625503855	17.72215831790871
29	17.32554608993186	25.70359622335239	36.13842452835697	20.832433158358782
30	20.281818022573468	29.907559328829542	30.454817055250306	19.355805593346684
31	29.81048640589814	25.953698891957593	24.344915909059946	19.89089879308432
32	31.764741946596754	25.87331569963545	24.901418675759892	17.460523678007895
33	28.64624388610889	26.740743768356275	24.89329277193556	19.719719573599278
34	20.278120006461798	26.957758924383356	29.0616381432845	23.702482925870346
35	20.293058045228683	25.785682449410167	31.504810340431575	22.41644916492957
36	29.911646609795074	26.213241233268906	25.123883539741023	18.751228617194997
37	21.52143194969141	32.77488424236408	26.92321166860326	18.78047213934125
38	20.74859524045863	33.508015936503114	24.027275788309986	21.716113034728263
39	21.413216320318263	31.94920482921978	25.881587577779985	20.755991272681975
40	25.07979929504135	26.26861415873053	24.723330005118832	23.92825654110929
41	18.667731306041972	24.536969456333242	26.788720661594546	30.00657857603024
42	22.18264696207976	26.061233307836485	23.69450299636621	28.061616733717536
43	21.597143963767227	26.762347967745516	25.107972338839485	26.532535729647773
44	20.136378941549935	29.770440784057268	28.496036310625566	21.597143963767227
45	18.597614974240397	35.86146258483541	23.00929953735872	22.53162290356547
46	21.769004396746524	33.48149726833389	24.715058126974302	20.034440207945284
47	21.12905297700029	27.164117955036016	27.127964981733747	24.578864086229952
48	22.51697681343898	23.059903968360555	31.85947928040499	22.563639937795475
49	20.969649019344516	24.061239146809292	32.11026316250445	22.85884867134174
50	19.470006169847935	33.19018118332623	26.09149865022412	21.248313996601716
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	584.0
1	1449.5
2	2315.0
3	5941.0
4	9567.0
5	7448.5
6	5330.0
7	5479.0
8	5628.0
9	6040.5
10	6453.0
11	6404.5
12	6356.0
13	6120.5
14	5885.0
15	5767.0
16	5649.0
17	5321.0
18	4993.0
19	5321.5
20	5650.0
21	5887.0
22	6124.0
23	7736.5
24	9349.0
25	11296.5
26	13244.0
27	16615.5
28	19987.0
29	22541.0
30	25095.0
31	32027.0
32	38959.0
33	44439.0
34	49919.0
35	54716.0
36	59513.0
37	63032.0
38	66551.0
39	86849.0
40	107147.0
41	138563.0
42	169979.0
43	193873.0
44	217767.0
45	215529.5
46	213292.0
47	194242.5
48	175193.0
49	163230.0
50	151267.0
51	145412.5
52	139558.0
53	127682.0
54	115806.0
55	104802.5
56	93799.0
57	87439.0
58	81079.0
59	77926.0
60	74773.0
61	68924.5
62	63076.0
63	52296.5
64	41517.0
65	33898.0
66	26279.0
67	20940.5
68	15602.0
69	12669.5
70	9737.0
71	7724.5
72	5712.0
73	4240.0
74	2768.0
75	2124.5
76	1481.0
77	1405.0
78	1329.0
79	888.5
80	448.0
81	377.5
82	307.0
83	180.5
84	54.0
85	39.0
86	24.0
87	14.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2055156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.10293426389631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45068513045092	15.97191900398979
2	7.2258057298696885	2.9051979518251128
3	2.652458877015588	1.5996661932699778
4	1.4575284169017044	1.1720239181094327
5	0.8722763775819866	0.8767657339240138
6	0.5793518537038533	0.6988003338405021
7	0.4084208688562595	0.5747320515034559
8	0.2987964767052118	0.4805348743590958
9	0.26069984793196993	0.47167487150257387
>10	3.9289204750208473	20.250675862711866
>50	1.9133306468072289	27.849898030117892
>100	0.9498122834962921	26.62393915683085
>500	0.0014347617438344785	0.18464276606724406
>1k	4.7825391461149276E-4	0.33952925194817624
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4539	0.22085914645895494	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2524	0.12281306139290643	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011045390228284373	0.0
2	0.0	0.0	0.0	0.04505740683432304	0.0
3	0.0	0.0	0.0	0.06559112787545082	0.0
4	0.0	0.0	0.0	0.11108645767036662	0.0
5	0.0	0.0	0.0	0.20572647526513801	0.0
6	0.0	0.0	0.0	0.31116859255453116	0.0
7	0.0	0.0	0.0	0.3865399998832205	0.0
8	0.0	0.0	0.0	0.596159123686961	0.0
9	0.0	0.0	0.0	0.6832571347381902	0.0
10	0.0	0.0	0.0	0.8151692620900798	0.0
11	0.0	0.0	0.0	0.9128747404090006	0.0
12	0.0	0.0	0.0	1.0156406618281046	0.0
13	0.0	0.0	0.0	1.0535453269727457	0.0
14	0.0	0.0	0.0	1.067607519818447	0.0
15	0.0	0.0	0.0	1.0885791638201674	0.0
16	0.0	0.0	0.0	1.139378227248929	0.0
17	0.0	0.0	0.0	1.1957729729519315	0.0
18	0.0	0.0	0.0	1.2989281592248958	0.0
19	0.0	0.0	0.0	1.342623139070708	0.0
20	4.865810673253028E-5	0.0	0.0	1.3898215026012624	0.0
21	4.865810673253028E-5	0.0	0.0	1.4513740076179131	0.0
22	4.865810673253028E-5	0.0	0.0	1.5193007246165255	0.0
23	9.731621346506056E-5	0.0	0.0	1.5915093550076005	0.0
24	9.731621346506056E-5	0.0	0.0	1.6431842643575476	0.0
25	9.731621346506056E-5	0.0	0.0	1.6902366535679043	0.0
26	9.731621346506056E-5	0.0	0.0	1.73977060622162	0.0
27	9.731621346506056E-5	0.0	0.0	1.7895478494089987	0.0
28	9.731621346506056E-5	0.0	0.0	1.8444828519100254	0.0
29	9.731621346506056E-5	0.0	0.0	1.9029212380957943	0.0
30	9.731621346506056E-5	0.0	0.0	1.9845208830862475	0.0
31	9.731621346506056E-5	0.0	0.0	2.061643982257308	0.0
32	9.731621346506056E-5	0.0	0.0	2.130251912750176	0.0
33	1.4597432019759085E-4	0.0	0.0	2.198713868922846	0.0
34	1.4597432019759085E-4	0.0	0.0	2.272333584409164	0.0
35	1.4597432019759085E-4	0.0	0.0	2.3659031236558197	0.0
36	1.4597432019759085E-4	0.0	0.0	2.444826572775984	0.0
37	1.4597432019759085E-4	0.0	0.0	2.5262802434462395	0.0
38	1.4597432019759085E-4	0.0	0.0	2.6075879397962978	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGCG	20	7.857931E-4	44.000004	1
TTTCGCG	25	4.444068E-5	44.0	1
ATATCGT	25	4.444068E-5	44.0	26
CGCGTAA	35	1.4468242E-7	44.0	31
GCTAACG	25	4.444068E-5	44.0	1
CGATCGA	25	4.444068E-5	44.0	41
GCGTATC	25	4.444068E-5	44.0	27
CGTTTTT	4520	0.0	41.128315	1
ACGGGTA	140	0.0	40.857143	5
CGGTCTA	165	0.0	40.0	31
CGGGTAT	245	0.0	39.510204	6
ACGGGAT	770	0.0	39.42857	5
CGTATCT	45	2.352499E-8	39.11111	28
ACCGGGT	85	0.0	38.823532	4
TGCGTAG	170	0.0	38.823532	1
TATGGGC	445	0.0	38.5618	4
TGCGAAC	40	4.1266503E-7	38.500004	11
AACGGGT	120	0.0	38.5	4
GTAGTAG	450	0.0	38.133335	1
TCGATAG	75	0.0	38.13333	1
>>END_MODULE