FastQCFastQC Report
Thu 26 May 2016
SRR1546548_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546548_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences331384
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14950.4511382565241533No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA4950.1493735364411076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCGA207.848363E-444.016
CGCATGG302.5222453E-644.02
CGACAGG454.783942E-1044.02
ACGGGTC302.5222453E-644.05
CGTTAGG207.848363E-444.02
TGTTACG207.848363E-444.01
AACGAGC302.5222453E-644.015
GCGATAA207.848363E-444.09
CGTAAGG254.4359622E-544.02
TGTAGCG254.4359622E-544.01
CGATCAC207.848363E-444.034
AATACGG207.848363E-444.02
AATCGAT207.848363E-444.017
CGTTTTT11400.042.4561421
GATGAAT2250.042.04444520
ATAGCGG700.040.8571432
ATCCGGG700.040.8571433
TAACGGG650.040.6153873
CGACCCG603.6379788E-1240.33333232
CGATGAA2400.040.33333219
Produced by FastQC (version 0.11.5)