##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546548_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	331384
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.628219829563285	31.0	30.0	31.0	28.0	33.0
2	30.94471972092799	31.0	31.0	33.0	28.0	34.0
3	31.09821838109263	31.0	31.0	34.0	28.0	34.0
4	34.92106740216788	35.0	35.0	37.0	32.0	37.0
5	34.707354609757864	35.0	35.0	37.0	32.0	37.0
6	34.72487205175869	35.0	35.0	37.0	32.0	37.0
7	35.42165584337204	35.0	35.0	37.0	35.0	37.0
8	34.40852304275403	35.0	35.0	37.0	33.0	37.0
9	36.548756125823815	39.0	37.0	39.0	32.0	39.0
10	35.97805868720276	38.0	35.0	39.0	30.0	39.0
11	35.62769475895034	37.0	35.0	39.0	30.0	39.0
12	34.72576527533013	37.0	33.0	39.0	29.0	39.0
13	34.10318241073799	37.0	33.0	39.0	25.0	39.0
14	34.956621321488065	37.0	33.0	40.0	25.0	41.0
15	35.4640477512493	37.0	33.0	40.0	27.0	41.0
16	35.73078362262511	37.0	34.0	40.0	30.0	41.0
17	35.654895830818624	37.0	34.0	40.0	29.0	41.0
18	35.594856722110904	37.0	34.0	40.0	29.0	41.0
19	35.58862829828839	37.0	34.0	40.0	29.0	41.0
20	35.36442012891389	36.0	34.0	40.0	29.0	41.0
21	35.13591784757261	36.0	33.0	40.0	28.0	41.0
22	35.05688566738286	36.0	33.0	40.0	27.0	41.0
23	35.01602974193081	36.0	33.0	40.0	28.0	41.0
24	34.97755775776742	36.0	33.0	40.0	27.0	41.0
25	34.829518021389084	35.0	33.0	39.0	27.0	41.0
26	34.6155698524974	35.0	33.0	39.0	27.0	41.0
27	34.39712840692369	35.0	33.0	39.0	25.0	41.0
28	34.49568476450281	36.0	33.0	39.0	25.0	41.0
29	34.416553001955435	36.0	33.0	39.0	25.0	41.0
30	34.45563153320619	36.0	33.0	39.0	26.0	41.0
31	34.3368629746759	35.0	33.0	39.0	25.0	41.0
32	34.024334307027495	35.0	33.0	39.0	24.0	41.0
33	33.833824203944665	35.0	32.0	39.0	23.0	41.0
34	33.74622492335176	35.0	32.0	39.0	23.0	41.0
35	33.51614441252444	35.0	32.0	39.0	23.0	41.0
36	33.40354694251985	35.0	32.0	39.0	22.0	41.0
37	33.406296019119814	35.0	32.0	39.0	23.0	41.0
38	33.471338386886515	35.0	32.0	39.0	23.0	41.0
39	33.40193250126741	35.0	32.0	39.0	23.0	41.0
40	33.319873620935226	35.0	32.0	39.0	22.0	41.0
41	33.41985732564034	35.0	32.0	39.0	23.0	41.0
42	33.25221797069261	35.0	32.0	39.0	22.0	41.0
43	33.283305772155565	35.0	32.0	39.0	23.0	41.0
44	32.94384460323975	35.0	31.0	39.0	21.0	41.0
45	32.89019687130338	35.0	31.0	39.0	21.0	41.0
46	32.88983173599208	35.0	31.0	39.0	21.0	41.0
47	32.826865509499555	35.0	31.0	39.0	21.0	40.0
48	32.91994785505637	35.0	31.0	39.0	22.0	40.0
49	32.97525529295319	35.0	31.0	39.0	22.0	40.0
50	32.356809622673396	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	3.0
14	5.0
15	13.0
16	43.0
17	113.0
18	286.0
19	565.0
20	928.0
21	1569.0
22	2359.0
23	3332.0
24	4532.0
25	5877.0
26	7375.0
27	8963.0
28	10066.0
29	11512.0
30	13443.0
31	16417.0
32	19627.0
33	24330.0
34	35449.0
35	38686.0
36	25568.0
37	29941.0
38	34980.0
39	35400.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.30466166139584	23.30800521449436	27.820293073896146	13.567040050213649
2	31.59506795741496	25.880247688482243	29.037008425270983	13.487675928831807
3	29.04907901407431	26.397170653984503	31.640936194867585	12.912814137073605
4	25.928228278975446	27.80007483765058	31.259203823962533	15.012493059411439
5	24.522004683388456	31.280930883808512	29.659850807522393	14.53721362528064
6	20.22849624604688	41.87951138256524	27.712261304106416	10.179731067281462
7	87.20547763319895	3.8822031238683823	7.542609178475726	1.3697100644569442
8	85.99479757622576	6.254677353161288	6.303563237814741	1.446961832798204
9	84.25391690606668	4.575960215339305	7.989522728918717	3.1806001496753016
10	51.610820075803296	24.535282331072107	13.304202979021317	10.549694614103275
11	44.78490210752481	21.342309827873404	21.628986311952296	12.243801752649494
12	39.50794244743258	21.917473384351688	23.596492286893756	14.97809188132197
13	22.15164280713613	37.46921999855153	24.837047051155157	15.542090143157184
14	16.521920189266833	39.845918933925596	28.038167201796107	15.593993675011466
15	15.348055428143784	23.853897593124593	45.63648214759916	15.161564831132463
16	17.81166260290177	20.15486565434662	43.54404557854332	18.48942616420829
17	18.733553822755475	20.089080945368515	28.268111918499383	32.909253313376624
18	22.298602225816573	22.7111145981701	34.788040460613665	20.202242715399656
19	28.813400767689444	24.15566231320764	26.1765806436038	20.85435627549912
20	31.875709147092195	23.642058759626295	25.541365907828983	18.940866185452528
21	23.450739927093643	28.313980155952006	28.911172536996354	19.324107379957994
22	23.32369697993868	23.036115201699538	28.231598387369335	25.408589430992446
23	20.772879801076698	29.466721386669242	26.698633609347468	23.0617652029066
24	20.129517417859645	24.493337034980566	37.46590058663062	17.911244960529174
25	18.839171474784543	24.222654081066075	35.826412862419424	21.111761581729958
26	18.17227144340101	33.017586847886434	27.858918958066774	20.951222750645776
27	18.764032059483863	32.64792506578471	29.86595611133911	18.72208676339232
28	16.53640489583082	27.810033073413322	38.10986649928784	17.543695531468025
29	17.369275523260026	25.245938246867684	37.114646452453954	20.270139777418343
30	20.17749800835285	29.33485020399295	31.1369891123289	19.350662675325303
31	29.351145498877436	25.782475919175337	25.212442362938464	19.653936219008763
32	30.238635540641674	25.887490041764238	26.97173068102262	16.90214373657147
33	28.355623687323465	26.314185355961662	26.30211476715834	19.028076189556526
34	20.197112715158244	27.734893658112643	28.45762016271154	23.610373464017577
35	20.52754508364918	25.726649445959975	30.97041498684306	22.77539048354779
36	29.749474929387056	25.15148588948169	26.645824783332934	18.453214397798327
37	21.884581029862638	31.5018226589093	27.465115998358403	19.148480312869662
38	20.845303333896627	32.672368008111434	23.990295246602127	22.49203341138981
39	21.071626873958913	30.640887912512373	26.956944209738552	21.330541003790167
40	24.227784081307487	25.4200564903556	26.148818289356157	24.20334113898076
41	18.703980880187334	24.671378219829563	27.64044130072665	28.98419959925645
42	22.53578929580185	25.094754122106078	25.623445911691572	26.7460106704005
43	22.064734567752215	25.615298264249333	26.229087704898248	26.090879463100208
44	20.6464403833619	28.928976655481254	28.60789899331289	21.816683967843954
45	19.439079738309633	34.848695169350364	23.749788764695943	21.962436327644063
46	22.358653405113103	31.57515148588948	26.046520049248002	20.019675059749414
47	21.973299857567053	28.08041426260773	26.800931849455615	23.145354030369603
48	22.15164280713613	25.396820606909206	30.47099437510562	21.980542210849045
49	21.5755739564976	24.540412331313522	31.8008714965116	22.08314221567728
50	20.535089201651257	31.74836443521715	26.80696714385728	20.909579219274317
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	116.0
1	284.0
2	452.0
3	1602.0
4	2752.0
5	2109.5
6	1467.0
7	1480.5
8	1494.0
9	1551.0
10	1608.0
11	1600.5
12	1593.0
13	1611.0
14	1629.0
15	1551.5
16	1474.0
17	1377.5
18	1281.0
19	1277.0
20	1273.0
21	1280.0
22	1287.0
23	1466.0
24	1645.0
25	1869.0
26	2093.0
27	2541.0
28	2989.0
29	3613.0
30	4237.0
31	5035.0
32	5833.0
33	6554.5
34	7276.0
35	7931.0
36	8586.0
37	9756.0
38	10926.0
39	13336.5
40	15747.0
41	20838.0
42	25929.0
43	29637.0
44	33345.0
45	33410.0
46	33475.0
47	30563.0
48	27651.0
49	26107.0
50	24563.0
51	23332.5
52	22102.0
53	20418.0
54	18734.0
55	17188.5
56	15643.0
57	14849.5
58	14056.0
59	12872.0
60	11688.0
61	10723.5
62	9759.0
63	8559.0
64	7359.0
65	5761.0
66	4163.0
67	3476.0
68	2789.0
69	2257.5
70	1726.0
71	1373.0
72	1020.0
73	887.0
74	754.0
75	581.5
76	409.0
77	310.0
78	211.0
79	165.5
80	120.0
81	95.0
82	70.0
83	48.5
84	27.0
85	23.5
86	20.0
87	12.5
88	5.0
89	5.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	331384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.675833406188175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.05973962650603	21.49140901263619
2	6.146891084243341	3.771220137324643
3	3.204135611397797	2.9486859067822118
4	2.4232608209767763	2.9734218097610556
5	1.9723489981545748	3.025172464312594
6	1.775410798540458	3.2677323530144764
7	1.6734444204384067	3.5934011579118583
8	1.4845071332699218	3.6430794808388893
9	1.3185622272476727	3.640319569686784
>10	9.857727158616239	49.146920768648194
>50	0.06997676717399971	1.3597150173655361
>100	0.012995685332314232	0.6804708217262347
>500	0.0	0.0
>1k	9.996681024857102E-4	0.4584514999913118
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1495	0.4511382565241533	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	495	0.1493735364411076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.00965647104265746	0.0
2	0.0	0.0	0.0	0.04737706105303817	0.0
3	0.0	0.0	0.0	0.072725297540014	0.0
4	0.0	0.0	0.0	0.12221471163363348	0.0
5	0.0	0.0	0.0	0.2549911884701736	0.0
6	0.0	0.0	0.0	0.3603070757791565	0.0
7	0.0	0.0	0.0	0.4315235497187553	0.0
8	0.0	0.0	0.0	0.6005117929652608	0.0
9	0.0	0.0	0.0	0.6602612075417039	0.0
10	0.0	0.0	0.0	0.8295512155082925	0.0
11	0.0	0.0	0.0	0.951765927141926	0.0
12	0.0	0.0	0.0	1.0948024044612896	0.0
13	0.0	0.0	0.0	1.1418777007942447	0.0
14	0.0	0.0	0.0	1.1602853487193105	0.0
15	0.0	0.0	0.0	1.195290056248944	0.0
16	0.0	0.0	0.0	1.2791806484320305	0.0
17	0.0	0.0	0.0	1.3573377109335394	0.0
18	0.0	0.0	0.0	1.4771383048065085	0.0
19	0.0	0.0	0.0	1.5426212490645295	0.0
20	0.0	0.0	0.0	1.605991840281969	0.0
21	0.0	0.0	0.0	1.6838471380633948	0.0
22	0.0	0.0	0.0	1.765021847765734	0.0
23	0.0	0.0	0.0	1.871243029234966	0.0
24	0.0	0.0	0.0	1.9448736209352293	0.0
25	0.0	0.0	0.0	2.0010018588706755	0.0
26	0.0	0.0	0.0	2.0655795089684474	0.0
27	0.0	0.0	0.0	2.1244236293846415	0.0
28	0.0	0.0	0.0	2.201675397725901	0.0
29	0.0	0.0	0.0	2.2894889312700673	0.0
30	0.0	0.0	0.0	2.3884677594573063	0.0
31	0.0	0.0	0.0	2.4829201168432995	0.0
32	0.0	0.0	0.0	2.572242473987881	0.0
33	0.0	0.0	0.0	2.6612630664123795	0.0
34	0.0	0.0	0.0	2.757526012118871	0.0
35	0.0	0.0	0.0	2.8830601356734182	0.0
36	0.0	0.0	0.0	2.98234072858074	0.0
37	0.0	0.0	0.0	3.0707577915650726	0.0
38	0.0	0.0	0.0	3.1842213263162975	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCGA	20	7.848363E-4	44.0	16
CGCATGG	30	2.5222453E-6	44.0	2
CGACAGG	45	4.783942E-10	44.0	2
ACGGGTC	30	2.5222453E-6	44.0	5
CGTTAGG	20	7.848363E-4	44.0	2
TGTTACG	20	7.848363E-4	44.0	1
AACGAGC	30	2.5222453E-6	44.0	15
GCGATAA	20	7.848363E-4	44.0	9
CGTAAGG	25	4.4359622E-5	44.0	2
TGTAGCG	25	4.4359622E-5	44.0	1
CGATCAC	20	7.848363E-4	44.0	34
AATACGG	20	7.848363E-4	44.0	2
AATCGAT	20	7.848363E-4	44.0	17
CGTTTTT	1140	0.0	42.456142	1
GATGAAT	225	0.0	42.044445	20
ATAGCGG	70	0.0	40.857143	2
ATCCGGG	70	0.0	40.857143	3
TAACGGG	65	0.0	40.615387	3
CGACCCG	60	3.6379788E-12	40.333332	32
CGATGAA	240	0.0	40.333332	19
>>END_MODULE