FastQCFastQC Report
Thu 26 May 2016
SRR1546547_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546547_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2000906
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79710.3983695385990147No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT20600.1029533621269565No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT60150.041.3665851
TCGTTGA603.6379788E-1240.33333624
TCACGAC2150.038.8837225
TAGTACG850.038.823531
TAGGGAC12800.038.671885
ACGTTAG750.038.133331
CGGTCTA2150.037.86046631
GGGCGAT19650.037.618327
CGTTGAT651.0913936E-1137.2307725
AATAGGG14600.037.2191773
GTAGGGA19050.036.8398934
AATGCGG2150.036.8372082
TACGGGA5200.036.8076934
AGGGCGA11250.036.7644426
CCGGTTA301.3014398E-436.66666811
TAGGGAT15800.036.3417745
CGTTTGG2550.036.2352942
CTACGAA1100.036.011
GTTAGCG1350.035.8518561
GTTGATC4050.035.85185216
Produced by FastQC (version 0.11.5)