##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546547_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2000906
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.68212549714979	31.0	30.0	31.0	28.0	33.0
2	30.999897546411475	31.0	31.0	33.0	28.0	34.0
3	31.161234960562865	31.0	31.0	34.0	28.0	34.0
4	34.994569460034604	35.0	35.0	37.0	32.0	37.0
5	34.79621231582093	35.0	35.0	37.0	32.0	37.0
6	34.83476635084307	35.0	35.0	37.0	32.0	37.0
7	35.43262402131834	35.0	35.0	37.0	35.0	37.0
8	34.39112182181472	36.0	35.0	37.0	33.0	37.0
9	36.52815924386253	39.0	37.0	39.0	32.0	39.0
10	36.00697883858612	38.0	35.0	39.0	30.0	39.0
11	35.681519271769886	37.0	35.0	39.0	30.0	39.0
12	35.02104696572453	37.0	34.0	39.0	30.0	39.0
13	34.646180280333006	37.0	33.0	39.0	27.0	39.0
14	35.5094552167868	38.0	33.0	40.0	27.0	41.0
15	35.85156074298343	38.0	33.0	40.0	29.0	41.0
16	36.028289184999196	38.0	34.0	40.0	30.0	41.0
17	35.957502751253685	38.0	34.0	40.0	30.0	41.0
18	35.893411284688035	37.0	34.0	40.0	30.0	41.0
19	35.88413648617176	37.0	34.0	40.0	29.0	41.0
20	35.73654184654352	37.0	34.0	40.0	29.0	41.0
21	35.5296580648966	37.0	34.0	40.0	29.0	41.0
22	35.48319561238759	37.0	34.0	40.0	29.0	41.0
23	35.44134557045658	37.0	34.0	40.0	29.0	41.0
24	35.374428383942075	37.0	34.0	40.0	29.0	41.0
25	35.19641952195656	36.0	34.0	40.0	27.0	41.0
26	35.00296815542559	36.0	33.0	40.0	27.0	41.0
27	34.80162286484223	36.0	33.0	40.0	27.0	41.0
28	34.83443600049178	36.0	33.0	40.0	27.0	41.0
29	34.71089746344906	36.0	33.0	40.0	26.0	41.0
30	34.72825709953391	36.0	33.0	40.0	26.0	41.0
31	34.65057928758272	36.0	33.0	40.0	26.0	41.0
32	34.45298929584898	36.0	33.0	40.0	25.0	41.0
33	34.32156333181069	36.0	33.0	40.0	24.0	41.0
34	34.20968101450043	36.0	33.0	40.0	24.0	41.0
35	34.04458580263141	36.0	33.0	40.0	24.0	41.0
36	33.97212762618534	36.0	33.0	40.0	23.0	41.0
37	34.00594930496485	36.0	33.0	40.0	23.0	41.0
38	34.045899207658934	36.0	33.0	40.0	23.0	41.0
39	33.9787816119298	36.0	33.0	40.0	23.0	41.0
40	33.85460586354382	36.0	32.0	40.0	23.0	41.0
41	33.90091688465125	35.0	33.0	40.0	23.0	41.0
42	33.75088884735215	35.0	32.0	40.0	23.0	41.0
43	33.78868172717759	35.0	32.0	40.0	23.0	41.0
44	33.494782363589295	35.0	32.0	39.0	23.0	41.0
45	33.400803436043475	35.0	32.0	39.0	23.0	41.0
46	33.36113490588763	35.0	32.0	39.0	23.0	41.0
47	33.30307070896884	35.0	32.0	39.0	23.0	41.0
48	33.34863256944604	35.0	32.0	39.0	23.0	41.0
49	33.35485325147708	35.0	32.0	39.0	23.0	41.0
50	32.78448962619933	35.0	31.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	2.0
12	4.0
13	17.0
14	23.0
15	93.0
16	246.0
17	659.0
18	1410.0
19	2694.0
20	4683.0
21	7405.0
22	11432.0
23	16089.0
24	22566.0
25	30483.0
26	39563.0
27	47672.0
28	55530.0
29	64784.0
30	77856.0
31	93668.0
32	114429.0
33	142431.0
34	203742.0
35	219886.0
36	171102.0
37	203022.0
38	238079.0
39	231330.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.65954622555982	23.258164051684588	28.425673170053965	12.656616552701628
2	32.570345633428055	24.881928486395662	28.838885984648954	13.708839895527325
3	30.797548710434175	25.531684147081375	29.97342203981596	13.69734510266849
4	27.75567667846466	27.42842492350965	29.232807538185202	15.583090859840492
5	23.803267119994644	31.674051654600465	28.847082271730905	15.675598953673987
6	21.29480345403532	41.0100224598257	27.208224674222574	10.486949411916402
7	88.02227590901322	3.975099280026148	6.35432149236396	1.6483033185966758
8	86.12723436283363	6.093189785027383	6.139868639506304	1.6397072126326775
9	84.46978518731015	4.811220517105752	7.842597303421551	2.87639699216255
10	49.03153871296303	25.634587531848073	13.40457772628999	11.929296028898909
11	40.27120714316415	23.265810587803728	21.161363902152324	15.301618366879804
12	36.10699353193004	22.63754519202801	25.26540477163845	15.990056504403505
13	23.6741755984539	32.2051610620389	26.174742841492805	17.945920498014402
14	18.194957684169072	34.89784127790111	29.12980419869799	17.77739683923183
15	17.769850257833202	23.803666938876688	40.933007347671506	17.493475455618604
16	21.054062509683114	21.147670105442234	38.57507549080267	19.223191894071988
17	21.335085206401498	20.41640137017931	28.15834427004567	30.09016915337352
18	24.026665920338086	23.236673786774592	32.10490647736575	20.63175381552157
19	29.17563343805256	25.38425093432675	25.495350606175403	19.944765021445285
20	31.126499695637875	24.497702540749042	24.507947899601483	19.867849864011603
21	25.724846644470055	26.942495049742465	27.568411509586156	19.76424679620132
22	24.41149159430778	23.22417944671064	27.1240627995518	25.24026615942978
23	22.65623672476368	28.61333815781451	26.6121446984516	22.11828041897021
24	22.39445531174378	24.270605415746665	33.516117198908894	19.818822073600657
25	21.975145259197586	24.942201182864164	31.62567356987285	21.456979988065406
26	20.312248551406213	30.469997091317637	26.803907829753122	22.41384652752303
27	20.95715640814711	29.509182340399796	28.780912246752223	20.75274900470087
28	18.754604164313566	27.637880040341727	33.991451872301845	19.616063923042862
29	20.939964196219112	26.07248916240943	32.32260785864003	20.664938782731422
30	22.764237800276472	27.172340929558906	30.22335881845524	19.840062451709375
31	29.84912834485978	24.688466124845444	25.15785349236796	20.30455203792682
32	29.546265541709605	25.845891811009615	25.6106983536458	18.99714429363498
33	28.21226984176168	25.61344710845987	25.429430467998	20.744852581780453
34	21.87574028964879	26.344016160679214	28.48114803993791	23.29909550973409
35	22.014327509638136	25.749285573635145	30.059832895698246	22.176554021028476
36	29.15099459944645	26.460263500634213	24.94225116022442	19.44649073969492
37	23.045160542274353	30.967421757943654	25.458417336946365	20.529000362835635
38	22.50735416856164	31.893002469881143	23.71655639995082	21.883086961606395
39	23.069999290321487	29.20462030700093	24.988730105262317	22.73665029741527
40	25.423383207407046	24.71390460121565	25.883274876480954	23.97943731489635
41	20.112239155662483	24.895322418944218	27.05294501590779	27.939493409485504
42	22.4876131112606	26.469909131163583	24.406193994120663	26.636283763455154
43	22.12302826819451	26.023961145601042	25.3674585412808	26.48555204492365
44	21.64584443247209	27.068737861748627	28.59759528933393	22.68782241644535
45	21.68107847145243	31.08012070532049	24.521091945348758	22.71770887787832
46	22.96034896192025	29.53357129220463	26.33846867369082	21.1676110721843
47	22.09464112756921	27.150850664648914	26.669418753304754	24.08508945447712
48	22.547835830368843	25.42023463371093	29.593544124511595	22.438385411408632
49	22.192146957428285	25.30018901437649	30.06932859414685	22.438335434048376
50	20.709318678638578	30.19032378332615	27.084630662309973	22.015726875725296
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	534.0
1	1577.5
2	2621.0
3	8817.0
4	15013.0
5	11081.5
6	7150.0
7	7231.5
8	7313.0
9	7584.0
10	7855.0
11	7745.5
12	7636.0
13	7388.5
14	7141.0
15	6953.0
16	6765.0
17	6835.5
18	6906.0
19	6672.5
20	6439.0
21	7074.5
22	7710.0
23	8886.0
24	10062.0
25	11986.5
26	13911.0
27	15794.0
28	17677.0
29	21076.5
30	24476.0
31	28635.0
32	32794.0
33	39586.0
34	46378.0
35	49777.0
36	53176.0
37	61196.0
38	69216.0
39	79907.0
40	90598.0
41	111359.0
42	132120.0
43	147478.0
44	162836.0
45	159593.0
46	156350.0
47	151269.0
48	146188.0
49	148547.0
50	150906.0
51	141153.5
52	131401.0
53	123248.5
54	115096.0
55	109361.0
56	103626.0
57	101955.5
58	100285.0
59	98964.0
60	97643.0
61	91173.5
62	84704.0
63	73719.0
64	62734.0
65	52286.0
66	41838.0
67	34736.5
68	27635.0
69	23174.0
70	18713.0
71	15465.0
72	12217.0
73	9865.0
74	7513.0
75	5769.0
76	4025.0
77	3081.0
78	2137.0
79	1557.0
80	977.0
81	646.5
82	316.0
83	229.0
84	142.0
85	105.5
86	69.0
87	60.0
88	51.0
89	29.0
90	7.0
91	6.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2000906.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.534401683609477
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.72040004298685	17.513827135794852
2	6.691279542353952	3.0156796196944513
3	2.732916206073462	1.8475389466591638
4	1.4659629324098615	1.321383902888235
5	0.9233059646783344	1.0403073742467068
6	0.6995011789759037	0.9457704327120852
7	0.518872740770451	0.8184740734237701
8	0.43682259234781295	0.7874828608352971
9	0.3880761667872358	0.7870557803597099
>10	6.466330538030736	36.78234442079369
>50	1.7136426653029768	26.242713373548014
>100	0.23826506345949197	7.517121731789176
>500	0.00286270265224705	0.4849113817002596
>1k	0.0015414552742868733	0.4998477638013421
>5k	2.2020789632669615E-4	0.395541201753251
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7971	0.3983695385990147	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAAT	2060	0.1029533621269565	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007946400280672856	0.0
2	0.0	0.0	0.0	0.036933269229039244	0.0
3	0.0	0.0	0.0	0.0582236246980118	0.0
4	0.0	0.0	0.0	0.08671072004382015	0.0
5	0.0	0.0	0.0	0.15363040542634188	0.0
6	0.0	0.0	0.0	0.2484374578316023	0.0
7	0.0	0.0	0.0	0.31605682625770526	0.0
8	0.0	0.0	0.0	0.4780834282070222	0.0
9	0.0	0.0	0.0	0.5655937860149353	0.0
10	0.0	0.0	0.0	0.6930360546672357	0.0
11	0.0	0.0	0.0	0.7890925410788913	0.0
12	0.0	0.0	0.0	0.8918959711250803	0.0
13	0.0	0.0	0.0	0.9304285158823054	0.0
14	0.0	0.0	0.0	0.9515689392705104	0.0
15	0.0	0.0	0.0	0.9748583891497152	0.0
16	0.0	0.0	0.0	1.0186885340940555	0.0
17	0.0	0.0	0.0	1.0661170489768135	0.0
18	0.0	0.0	0.0	1.1513784255732153	0.0
19	0.0	0.0	0.0	1.1919600421009282	0.0
20	0.0	0.0	0.0	1.237739304095245	0.0
21	0.0	0.0	0.0	1.2928643324573967	0.0
22	0.0	0.0	0.0	1.3489889080246649	0.0
23	0.0	0.0	0.0	1.4125101329097918	0.0
24	0.0	0.0	0.0	1.4642367007745491	0.0
25	0.0	0.0	0.0	1.5069673437932616	0.0
26	0.0	0.0	0.0	1.5500478283337649	0.0
27	0.0	0.0	0.0	1.5963768412908952	0.0
28	0.0	0.0	0.0	1.6484532506774432	0.0
29	0.0	0.0	0.0	1.7059771923318736	0.0
30	0.0	0.0	0.0	1.7844416479334861	0.0
31	0.0	0.0	0.0	1.850061921949357	0.0
32	0.0	0.0	0.0	1.9121837807473214	0.0
33	0.0	0.0	0.0	1.9798031491734245	0.0
34	0.0	0.0	0.0	2.046522925114923	0.0
35	0.0	0.0	0.0	2.1359324226125564	0.0
36	0.0	0.0	0.0	2.2094990969091	0.0
37	0.0	0.0	0.0	2.2864642317030386	0.0
38	0.0	0.0	0.0	2.378722438735253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6015	0.0	41.366585	1
TCGTTGA	60	3.6379788E-12	40.333336	24
TCACGAC	215	0.0	38.88372	25
TAGTACG	85	0.0	38.82353	1
TAGGGAC	1280	0.0	38.67188	5
ACGTTAG	75	0.0	38.13333	1
CGGTCTA	215	0.0	37.860466	31
GGGCGAT	1965	0.0	37.61832	7
CGTTGAT	65	1.0913936E-11	37.23077	25
AATAGGG	1460	0.0	37.219177	3
GTAGGGA	1905	0.0	36.839893	4
AATGCGG	215	0.0	36.837208	2
TACGGGA	520	0.0	36.807693	4
AGGGCGA	1125	0.0	36.764442	6
CCGGTTA	30	1.3014398E-4	36.666668	11
TAGGGAT	1580	0.0	36.341774	5
CGTTTGG	255	0.0	36.235294	2
CTACGAA	110	0.0	36.0	11
GTTAGCG	135	0.0	35.851856	1
GTTGATC	405	0.0	35.851852	16
>>END_MODULE