FastQCFastQC Report
Thu 26 May 2016
SRR1546546_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546546_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1770630
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36400.2055765462010697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACTA302.5284953E-644.00000430
TAACGCG302.5284953E-644.0000041
ACTATCG207.857636E-444.025
TCCGCAT207.857636E-444.044
CCCGTTA207.857636E-444.019
CTACCGT207.857636E-444.011
CGTCGCA207.857636E-444.044
TAAGCGA207.857636E-444.041
TAATGCG454.802132E-1044.01
ATAACGG1150.042.0869522
CGGTCTA1000.041.831
CGTTTTT36300.041.3333321
GCGAATG1350.040.740741
CTATGCG603.6379788E-1240.3333361
GTATACG1150.040.173911
TAGGGAC12750.040.0313725
AATACGG950.039.368422
TAGTCGG1700.038.8235322
ACGCACG404.1262138E-738.51
TACGAAC750.038.133338
Produced by FastQC (version 0.11.5)