##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546546_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1770630
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.689024245607495	31.0	30.0	31.0	28.0	33.0
2	30.99733880031401	31.0	31.0	33.0	28.0	34.0
3	31.17191112767772	31.0	31.0	34.0	28.0	34.0
4	34.98316983220661	35.0	35.0	37.0	32.0	37.0
5	34.76657630334965	35.0	35.0	37.0	32.0	37.0
6	34.80912782455962	35.0	35.0	37.0	32.0	37.0
7	35.429578172740776	35.0	35.0	37.0	35.0	37.0
8	34.412519272801205	36.0	35.0	37.0	33.0	37.0
9	36.58457837041053	39.0	37.0	39.0	32.0	39.0
10	36.06861173706534	38.0	35.0	39.0	31.0	39.0
11	35.6709154368784	37.0	35.0	39.0	30.0	39.0
12	34.566882409086034	35.0	33.0	39.0	27.0	39.0
13	33.83030729175491	35.0	33.0	39.0	23.0	39.0
14	34.685052213054114	36.0	33.0	40.0	24.0	41.0
15	35.2763897595771	36.0	33.0	40.0	27.0	41.0
16	35.59142056782049	36.0	34.0	40.0	30.0	41.0
17	35.562015779694235	36.0	34.0	40.0	29.0	41.0
18	35.518775238192056	36.0	34.0	40.0	29.0	41.0
19	35.48278352902639	36.0	34.0	40.0	29.0	41.0
20	35.30169261788177	36.0	34.0	40.0	29.0	41.0
21	35.05153871785748	36.0	33.0	40.0	28.0	41.0
22	34.990040832923874	35.0	33.0	40.0	27.0	41.0
23	35.0185939467873	35.0	33.0	40.0	29.0	41.0
24	34.967859462451216	35.0	33.0	40.0	29.0	41.0
25	34.79944144174672	35.0	33.0	39.0	27.0	41.0
26	34.56789052484144	35.0	33.0	39.0	27.0	41.0
27	34.3874304626037	35.0	33.0	39.0	26.0	41.0
28	34.480849754042346	35.0	33.0	39.0	26.0	41.0
29	34.436095626980226	35.0	33.0	39.0	26.0	41.0
30	34.48612301836069	36.0	33.0	39.0	27.0	41.0
31	34.372731174779595	35.0	33.0	39.0	26.0	41.0
32	34.13921203187566	35.0	33.0	39.0	25.0	41.0
33	33.99805718868426	35.0	33.0	39.0	24.0	41.0
34	33.968127163777865	35.0	33.0	39.0	24.0	41.0
35	33.786200391950885	35.0	32.0	39.0	24.0	41.0
36	33.6498585249318	35.0	32.0	39.0	23.0	41.0
37	33.64063243026494	35.0	32.0	39.0	23.0	41.0
38	33.72439357742724	35.0	32.0	39.0	23.0	41.0
39	33.67716744887413	35.0	32.0	39.0	23.0	41.0
40	33.54213867380537	35.0	32.0	39.0	23.0	41.0
41	33.65798444621406	35.0	32.0	39.0	23.0	41.0
42	33.5125768794158	35.0	32.0	39.0	23.0	41.0
43	33.53727147964284	35.0	32.0	39.0	23.0	41.0
44	33.26352710617125	35.0	32.0	39.0	23.0	41.0
45	33.15545370856701	35.0	31.0	39.0	23.0	40.0
46	33.15041708318508	35.0	31.0	38.0	23.0	40.0
47	33.11349858524932	35.0	31.0	38.0	23.0	40.0
48	33.19837684891818	35.0	32.0	38.0	23.0	40.0
49	33.249745006014805	35.0	32.0	38.0	24.0	40.0
50	32.642888124565836	35.0	31.0	38.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	2.0
13	8.0
14	18.0
15	87.0
16	205.0
17	578.0
18	1214.0
19	2388.0
20	4288.0
21	6937.0
22	10474.0
23	14781.0
24	20629.0
25	27867.0
26	35849.0
27	43901.0
28	51303.0
29	60803.0
30	72898.0
31	89207.0
32	109113.0
33	137532.0
34	207691.0
35	225657.0
36	133740.0
37	155525.0
38	180887.0
39	177045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.047542400162655	24.078152973800286	29.778892258687584	12.095412367349475
2	30.012424956089074	25.3985304665571	31.51827315701191	13.070771420341911
3	29.79888514257637	25.868306760870425	30.71211941512343	13.620688681429774
4	27.36794248374872	27.900069466799955	29.888796643002774	14.843191406448552
5	23.02338715598403	32.94341562042889	29.17899278787776	14.854204435709322
6	20.59402585520408	41.980538000598656	26.517171854085834	10.908264290111429
7	90.18716502035998	3.933571666581951	4.581363695407849	1.2978996176502149
8	89.16086364740234	5.361537983655535	4.143496947414197	1.3341014215279308
9	88.83894433054903	3.9297312256089643	5.127440515522723	2.1038839283192985
10	58.04335180133625	23.181579437827214	9.718292359216777	9.056776401619762
11	50.10606394334221	18.921005517810045	17.91300271654722	13.059927822300537
12	43.72743034964956	21.023590473447303	21.650034168629244	13.59894500827389
13	21.877580296278726	40.024962866324415	22.17035744339585	15.927099394001004
14	15.229099247160615	42.57879963628765	26.720037500776563	15.472063615775175
15	13.877715841254243	22.39988026860496	48.04323884718998	15.679165042950812
16	17.425436144197263	17.3448998379108	45.52475672500748	19.704907292884457
17	18.367021907456667	17.524892269983	26.532872480416575	37.575213342143755
18	23.329492892360346	22.4090860315255	32.88366287705506	21.37775819905909
19	31.726617079796455	23.28018840751597	23.735676002326855	21.25751851036072
20	33.13131484273959	23.390375177196816	22.57123170848794	20.907078271575656
21	24.05369840113406	27.72549883374844	26.22061074306885	22.000192022048648
22	23.469443079581843	22.890327171684653	24.68115868363238	28.95907106510112
23	21.21860580697266	30.07748654433733	24.4778412203566	24.226066428333418
24	20.963216482269022	23.32282859773075	36.946284655744	18.767670264256225
25	19.126130247426058	23.99287259337072	34.943946504916326	21.937050654286892
26	18.027933560371167	34.29508141170092	25.715592755121058	21.961392272806854
27	18.874694317841673	32.32007816426922	28.559100433179154	20.24612708470996
28	16.082185436821923	27.627906451376067	38.65059329165325	17.639314820148762
29	17.192411740453963	24.577636208581126	36.99214403912732	21.237808011837593
30	20.315593884662523	28.984259839718067	30.56889355766027	20.131252717959143
31	32.809621434178794	24.69375307094085	22.68102313865686	19.81560235622349
32	32.90986823898838	25.058538486301487	23.84942082761503	18.182172447095102
33	29.671868205102136	25.641099495659738	24.060870989421844	20.62616130981628
34	20.7024053585447	26.338647825915068	27.060876637129155	25.898070178411075
35	21.110847551436496	24.89961200250758	31.126096361182178	22.863444084873745
36	33.32949289236035	23.53331864929432	24.301463320964857	18.83572513738048
37	22.485725419765846	32.15646408340534	25.914109667180607	19.443700829648204
38	21.22120375233674	34.52409594325184	22.03656325714576	22.218137047265664
39	23.04360594816534	30.217945025217013	24.841553571327722	21.896895455289926
40	26.03124311685671	24.613160287581255	23.970168809971593	25.38542778559044
41	18.73960115890954	23.354342804538497	25.779072985321612	32.126983051230354
42	22.889084676075747	25.053342595573326	22.48758916317921	29.56998356517172
43	22.641206802098687	24.775418918689958	24.482246432060904	28.101127847150448
44	20.909958602305394	28.744853526710834	27.939942280431257	22.405245590552514
45	19.05124164845281	35.960420867148976	22.0378622298278	22.950475254570406
46	22.889875355099598	31.258986914262156	25.349451890005252	20.501685840632994
47	22.296301316480573	25.690234549284718	26.48266436240208	25.530799771832623
48	23.52541185905582	22.638100563076417	30.913121318400794	22.92336625946697
49	21.115986965091523	23.37377091769596	31.887407307003723	23.622834810208797
50	19.746417941636594	32.255863732117945	25.69187238440555	22.305845941839912
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	326.0
1	1033.0
2	1740.0
3	4497.0
4	7254.0
5	5688.5
6	4123.0
7	4395.5
8	4668.0
9	4948.5
10	5229.0
11	5340.5
12	5452.0
13	5335.0
14	5218.0
15	4843.5
16	4469.0
17	4559.0
18	4649.0
19	4081.0
20	3513.0
21	3901.0
22	4289.0
23	4973.5
24	5658.0
25	6681.5
26	7705.0
27	9122.5
28	10540.0
29	13194.0
30	15848.0
31	20333.5
32	24819.0
33	28000.5
34	31182.0
35	35852.0
36	40522.0
37	43174.0
38	45826.0
39	61847.5
40	77869.0
41	109190.0
42	140511.0
43	166235.5
44	191960.0
45	183457.5
46	174955.0
47	163491.0
48	152027.0
49	142689.0
50	133351.0
51	128645.5
52	123940.0
53	114122.5
54	104305.0
55	93502.0
56	82699.0
57	81045.5
58	79392.0
59	78329.0
60	77266.0
61	71356.5
62	65447.0
63	58747.5
64	52048.0
65	41673.5
66	31299.0
67	25477.5
68	19656.0
69	16371.5
70	13087.0
71	10742.5
72	8398.0
73	6373.5
74	4349.0
75	3420.5
76	2492.0
77	2090.5
78	1689.0
79	1075.5
80	462.0
81	345.5
82	229.0
83	156.0
84	83.0
85	70.5
86	58.0
87	36.0
88	14.0
89	10.5
90	7.0
91	6.0
92	5.0
93	3.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1770630.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.937268715935467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.23811537956352	15.19490752203029
2	6.971191917636654	2.6403066922928558
3	2.560762160331213	1.4548152344337482
4	1.3427224957412358	1.0170998685113322
5	0.8255241984459449	0.7816586788739048
6	0.5666699097972531	0.6438708213039298
7	0.41513249238429506	0.5503032892698201
8	0.29831066168924025	0.45193513289901266
9	0.2282053139617495	0.3889426817608263
>10	3.379539745337575	16.50245980014767
>50	2.013234958555634	27.968847082280295
>100	1.153173903272558	31.130981321734826
>500	0.005340141563875939	0.6701395306773132
>1k	0.0020767217192850873	0.6037323437842395
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3640	0.2055765462010697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010109396090656997	0.0
2	0.0	0.0	0.0	0.042583713141650145	0.0
3	0.0	0.0	0.0	0.059244449715637935	0.0
4	0.0	0.0	0.0	0.09482500578889999	0.0
5	0.0	0.0	0.0	0.17157734817550815	0.0
6	0.0	0.0	0.0	0.2636914544540644	0.0
7	0.0	0.0	0.0	0.32937429050676875	0.0
8	0.0	0.0	0.0	0.5277782484200538	0.0
9	0.0	0.0	0.0	0.6183674737240418	0.0
10	0.0	0.0	0.0	0.7649819555751343	0.0
11	0.0	0.0	0.0	0.8601458238028272	0.0
12	0.0	0.0	0.0	0.9736082637253407	0.0
13	0.0	0.0	0.0	1.0169261788177089	0.0
14	0.0	0.0	0.0	1.0321185114902605	0.0
15	0.0	0.0	0.0	1.052902074402896	0.0
16	0.0	0.0	0.0	1.1154221943601994	0.0
17	0.0	0.0	0.0	1.178732993341353	0.0
18	0.0	0.0	0.0	1.3022483522813915	0.0
19	0.0	0.0	0.0	1.3438719551797949	0.0
20	0.0	0.0	0.0	1.392442238073454	0.0
21	0.0	0.0	0.0	1.452759752178603	0.0
22	0.0	0.0	0.0	1.510196935554012	0.0
23	0.0	0.0	0.0	1.5761621569723772	0.0
24	5.6477073132161996E-5	0.0	0.0	1.625297210597358	0.0
25	5.6477073132161996E-5	0.0	0.0	1.677990319829665	0.0
26	5.6477073132161996E-5	0.0	0.0	1.7256569695532098	0.0
27	5.6477073132161996E-5	0.0	0.0	1.7692007929381068	0.0
28	5.6477073132161996E-5	0.0	0.0	1.8198607275376562	0.0
29	5.6477073132161996E-5	0.0	0.0	1.8698994143327516	0.0
30	5.6477073132161996E-5	0.0	0.0	1.950209812326686	0.0
31	5.6477073132161996E-5	0.0	0.0	2.0187729791091304	0.0
32	5.6477073132161996E-5	0.0	0.0	2.0794293556530725	0.0
33	5.6477073132161996E-5	0.0	0.0	2.1455640082908345	0.0
34	5.6477073132161996E-5	0.0	0.0	2.212432862879314	0.0
35	5.6477073132161996E-5	0.0	0.0	2.305563556474249	0.0
36	5.6477073132161996E-5	0.0	0.0	2.3820335134951964	0.0
37	5.6477073132161996E-5	0.0	0.0	2.459576534905655	0.0
38	5.6477073132161996E-5	0.0	0.0	2.536950125096717	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGACTA	30	2.5284953E-6	44.000004	30
TAACGCG	30	2.5284953E-6	44.000004	1
ACTATCG	20	7.857636E-4	44.0	25
TCCGCAT	20	7.857636E-4	44.0	44
CCCGTTA	20	7.857636E-4	44.0	19
CTACCGT	20	7.857636E-4	44.0	11
CGTCGCA	20	7.857636E-4	44.0	44
TAAGCGA	20	7.857636E-4	44.0	41
TAATGCG	45	4.802132E-10	44.0	1
ATAACGG	115	0.0	42.086952	2
CGGTCTA	100	0.0	41.8	31
CGTTTTT	3630	0.0	41.333332	1
GCGAATG	135	0.0	40.74074	1
CTATGCG	60	3.6379788E-12	40.333336	1
GTATACG	115	0.0	40.17391	1
TAGGGAC	1275	0.0	40.031372	5
AATACGG	95	0.0	39.36842	2
TAGTCGG	170	0.0	38.823532	2
ACGCACG	40	4.1262138E-7	38.5	1
TACGAAC	75	0.0	38.13333	8
>>END_MODULE