FastQCFastQC Report
Thu 26 May 2016
SRR1546545_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546545_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1416802
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153571.0839199831733721No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT119050.043.131461
TAGTACG1300.040.6153831
TGCGTTG452.3515895E-839.111111
AGGGCGA7700.038.2857136
CGGTCTA1100.038.031
GTTTTTT135800.037.8762862
TAGGGAT18800.037.3297845
TACGGGA4650.036.903234
TAGGGAC11000.036.65
GCGCGAC2850.036.2807049
GGGCGAT13800.036.1884047
CGTTAGG2250.036.177782
AGGGATT18250.036.043846
CTACGGG2200.036.03
ATAGGGA18950.035.989454
ACGGGAT5750.035.9652185
AGACGGG5600.035.753
CGGGATT5500.035.66
TAAGGGA15250.035.488524
CTAGGGA9700.035.3814434
Produced by FastQC (version 0.11.5)