##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546545_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1416802
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.601832154387132	31.0	30.0	31.0	28.0	34.0
2	30.942920746865124	31.0	31.0	33.0	28.0	34.0
3	31.056383319616998	31.0	31.0	34.0	28.0	34.0
4	34.898523576336004	35.0	35.0	37.0	32.0	37.0
5	34.706651317544726	35.0	35.0	37.0	32.0	37.0
6	34.74699711039369	35.0	35.0	37.0	32.0	37.0
7	35.40454064858745	35.0	35.0	37.0	33.0	37.0
8	34.36634123893106	35.0	35.0	37.0	32.0	37.0
9	36.4948489626638	39.0	37.0	39.0	32.0	39.0
10	35.91954768556227	38.0	35.0	39.0	30.0	39.0
11	35.61707634517738	37.0	35.0	39.0	30.0	39.0
12	35.26834095378182	37.0	34.0	39.0	30.0	39.0
13	35.07427149312325	37.0	34.0	39.0	28.0	39.0
14	36.026931074349136	38.0	34.0	40.0	29.0	41.0
15	36.23587558459121	38.0	34.0	40.0	30.0	41.0
16	36.32241767021786	38.0	34.0	40.0	30.0	41.0
17	36.21936445600726	38.0	34.0	40.0	30.0	41.0
18	36.071905601488425	38.0	34.0	40.0	30.0	41.0
19	35.98256001897231	37.0	34.0	40.0	30.0	41.0
20	35.7485611962716	37.0	34.0	40.0	29.0	41.0
21	35.57575017539501	37.0	34.0	40.0	29.0	41.0
22	35.52654569939907	37.0	34.0	40.0	29.0	41.0
23	35.44751489622403	37.0	34.0	40.0	29.0	41.0
24	35.351260091388916	37.0	34.0	40.0	28.0	41.0
25	35.18762184130175	37.0	33.0	40.0	27.0	41.0
26	34.99916784420124	36.0	33.0	40.0	27.0	41.0
27	34.786023029329435	36.0	33.0	40.0	26.0	41.0
28	34.78340586758065	36.0	33.0	40.0	26.0	41.0
29	34.60811390723615	36.0	33.0	40.0	25.0	41.0
30	34.567129351878386	36.0	33.0	40.0	25.0	41.0
31	34.43596211750125	36.0	33.0	40.0	24.0	41.0
32	34.06670798036705	36.0	33.0	40.0	23.0	41.0
33	33.76710154277027	36.0	32.0	40.0	22.0	41.0
34	33.54405273284482	36.0	32.0	40.0	21.0	41.0
35	33.281332183325546	36.0	31.0	40.0	18.0	41.0
36	33.21792388774155	36.0	31.0	40.0	18.0	41.0
37	33.230765484520774	36.0	31.0	40.0	18.0	41.0
38	33.247553292556056	36.0	31.0	40.0	18.0	41.0
39	33.1357627953659	36.0	31.0	40.0	18.0	41.0
40	33.044965351545244	36.0	31.0	40.0	18.0	41.0
41	33.070117066463766	36.0	31.0	40.0	18.0	41.0
42	32.91469238468043	36.0	31.0	40.0	18.0	41.0
43	32.958970978301835	36.0	31.0	40.0	18.0	41.0
44	32.57699099803642	35.0	31.0	40.0	15.0	41.0
45	32.55256556667763	35.0	31.0	39.0	16.0	41.0
46	32.507093440014906	35.0	30.0	39.0	17.0	41.0
47	32.471667883021055	35.0	30.0	39.0	17.0	41.0
48	32.49621683199205	35.0	31.0	39.0	17.0	41.0
49	32.52062744123738	35.0	31.0	39.0	18.0	41.0
50	31.99179137240066	35.0	30.0	39.0	15.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	4.0
12	6.0
13	7.0
14	26.0
15	100.0
16	240.0
17	647.0
18	1494.0
19	2967.0
20	4947.0
21	7343.0
22	10704.0
23	14974.0
24	20961.0
25	28424.0
26	36685.0
27	42918.0
28	45579.0
29	48341.0
30	54799.0
31	64045.0
32	75398.0
33	91596.0
34	122388.0
35	134101.0
36	121496.0
37	143760.0
38	171397.0
39	171451.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.293928156510226	23.368050016868978	27.27579435940943	18.06222746721137
2	34.89231381660952	24.07506482910103	27.966293102353045	13.066328251936405
3	27.97822137461692	24.727590728979774	34.585990138353836	12.708197758049467
4	25.124611625336495	26.274666467156315	34.57236791026551	14.028353997241675
5	22.39931902975857	30.406224723002932	33.55641790454841	13.638038342690088
6	19.465599286279947	40.06939572360852	30.546823056432725	9.918181933678806
7	84.08316758446134	3.165721109936322	11.486785027124467	1.264326278477868
8	82.65975062147004	5.504650614553057	10.567037595937894	1.268561168039006
9	80.83973625107814	4.4385171675364665	12.15434478494525	2.567401796440152
10	40.883553241737374	29.531790610120538	18.572037588879745	11.012618559262338
11	30.957677925355835	25.33127423592005	29.798235745008828	13.912812093715281
12	30.08507893128327	21.891132282421964	31.543221988675906	16.480566797618863
13	22.637672730557977	27.005678986901486	32.029034402831165	18.32761387970937
14	17.46419047968594	30.111546991040385	33.713744051744705	18.71051847752897
15	17.233812487560012	25.3266158574028	40.423079583456264	17.016492071580927
16	20.88915741225662	22.624262246947705	39.14788375510481	17.338696585690872
17	20.732043009538383	22.66061171568081	32.90304502675745	23.704300248023365
18	21.70451481576113	24.491848543409738	34.4573906586806	19.34624598214853
19	25.300500705109112	25.807275822592008	30.75701474165056	18.13520873064832
20	27.621996581032494	24.5505017638315	30.3808153856361	17.446686269499903
21	24.438559516432075	25.528761252454473	32.11359103106856	17.91908820004489
22	22.203667132033974	23.187079069622996	33.04067893749444	21.56857486084859
23	21.342784665747224	26.316874199782326	32.53418614598229	19.80615498848816
24	21.021074222086078	23.683408126188414	36.35179792236318	18.943719729362325
25	20.86628900862647	25.423453665367497	34.0199971485077	19.690260177498338
26	19.97611522287518	29.066023339888	31.532564183280375	19.425297253956444
27	19.590246202362785	27.97638625580709	33.03291497329902	19.4004525685311
28	18.793663475912652	26.751373868755124	35.791027962975775	18.66393469235645
29	19.98310279065106	25.491917713272567	35.52168898688737	19.003290509189004
30	21.014863050729744	26.09242505304199	33.64499767786889	19.247714218359377
31	24.588051117940264	24.916960873855345	30.63709678557766	19.857891222626733
32	25.48747107923337	26.13378580775578	30.433963249628388	17.94477986338246
33	25.17592437051896	26.398678149804983	29.374464462924248	19.050933016751813
34	21.07153998935631	27.214670786743667	30.786376642607788	20.927412581292234
35	21.236983008211453	26.90347698549268	31.285387795895264	20.574152210400605
36	23.932207887905296	27.9296613076492	28.479420554177647	19.65871025026786
37	22.127015630977372	30.10208907102051	28.33981036164545	19.43108493635667
38	21.826479635121913	30.14316749976355	27.15940547797081	20.870947387143723
39	22.072738463102112	28.63321762673966	28.04223878848279	21.251805121675435
40	23.06913739534529	26.603576223071396	28.92930698855592	21.397979393027395
41	19.66089827654111	26.611834257715618	29.775649667349423	23.951617798393848
42	21.390709499280774	27.829223843557532	27.40015894952153	23.379907707640164
43	21.436305143555696	27.577106751684425	27.76280665894035	23.223781445819526
44	21.01521595819317	28.698858414937302	29.44709281889777	20.838832807971755
45	21.198939583653893	29.406437879110843	27.80423799514682	21.59038454208845
46	21.53483690734485	29.946950950097474	28.11613761132466	20.402074531233016
47	21.29514215818442	27.830494310425873	29.334868245527602	21.539495285862102
48	21.89847275766127	25.52607915573242	31.153541567558484	21.42190651904783
49	21.326903829892956	26.028760546639546	31.04908095838374	21.59525466508376
50	19.680237605536977	28.880746921588198	29.687493382985057	21.751522089889765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	463.0
1	1166.5
2	1870.0
3	15233.0
4	28596.0
5	21330.5
6	14065.0
7	13845.0
8	13625.0
9	13580.5
10	13536.0
11	13344.5
12	13153.0
13	12596.0
14	12039.0
15	11199.5
16	10360.0
17	9707.0
18	9054.0
19	8781.0
20	8508.0
21	8384.5
22	8261.0
23	8931.0
24	9601.0
25	10614.0
26	11627.0
27	14384.5
28	17142.0
29	19576.0
30	22010.0
31	26277.5
32	30545.0
33	35293.5
34	40042.0
35	43410.0
36	46778.0
37	51525.5
38	56273.0
39	62556.0
40	68839.0
41	79376.0
42	89913.0
43	97311.0
44	104709.0
45	105200.5
46	105692.0
47	101886.5
48	98081.0
49	97107.0
50	96133.0
51	91175.0
52	86217.0
53	81258.5
54	76300.0
55	72299.5
56	68299.0
57	65187.5
58	62076.0
59	60342.0
60	58608.0
61	53890.5
62	49173.0
63	41411.0
64	33649.0
65	27098.0
66	20547.0
67	16231.5
68	11916.0
69	10040.5
70	8165.0
71	6619.0
72	5073.0
73	3918.0
74	2763.0
75	2122.0
76	1481.0
77	1149.5
78	818.0
79	658.0
80	498.0
81	334.0
82	170.0
83	122.5
84	75.0
85	51.0
86	27.0
87	25.0
88	23.0
89	13.5
90	4.0
91	3.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1416802.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.274399916299256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.82340469046906	23.713314813051305
2	6.319735934644011	3.9529189797092807
3	2.759363364860067	2.588923001610568
4	1.7648220292443648	2.207749996947322
5	1.32982344660444	2.0794715143589344
6	1.1046308468505397	2.072800011857046
7	0.9562257996906773	2.093377164886652
8	0.8301481447764862	2.076990805561098
9	0.7794741246494428	2.1939826948824575
>10	8.135327423260973	51.33269304348942
>50	0.1738624173780108	3.295994883012431
>100	0.022056448864162477	1.1539404244322218
>500	9.002629662818672E-4	0.15689476304803787
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	2.250657415704668E-4	1.0809479031533054
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15357	1.0839199831733721	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009810827483303948	0.0
2	0.0	0.0	0.0	0.03980796187470091	0.0
3	0.0	0.0	0.0	0.07072265567101119	0.0
4	0.0	0.0	0.0	0.09528501512561388	0.0
5	0.0	0.0	0.0	0.16311382959651383	0.0
6	0.0	0.0	0.0	0.2685625796688599	0.0
7	0.0	0.0	0.0	0.3442965213205515	0.0
8	0.0	0.0	0.0	0.5200444381077949	0.0
9	0.0	0.0	0.0	0.6293751702778511	0.0
10	0.0	0.0	0.0	0.7701852481857028	0.0
11	7.058149268563992E-5	0.0	0.0	0.8500129164131615	0.0
12	7.058149268563992E-5	0.0	0.0	0.940074901080038	0.0
13	7.058149268563992E-5	0.0	0.0	0.979459373998625	0.0
14	7.058149268563992E-5	0.0	0.0	1.0023277776287725	0.0
15	7.058149268563992E-5	0.0	0.0	1.0244197848393777	0.0
16	7.058149268563992E-5	0.0	0.0	1.0647923986555636	0.0
17	7.058149268563992E-5	0.0	0.0	1.1064354793400912	0.0
18	7.058149268563992E-5	0.0	0.0	1.1795579057624141	0.0
19	7.058149268563992E-5	0.0	0.0	1.2175307488272884	0.0
20	7.058149268563992E-5	0.0	0.0	1.258397433092274	0.0
21	7.058149268563992E-5	0.0	0.0	1.3001110952694872	0.0
22	7.058149268563992E-5	0.0	0.0	1.350506281047034	0.0
23	7.058149268563992E-5	0.0	0.0	1.4068303122101748	0.0
24	7.058149268563992E-5	0.0	0.0	1.4512260711094422	0.0
25	7.058149268563992E-5	0.0	0.0	1.491881010896371	0.0
26	7.058149268563992E-5	0.0	0.0	1.530277342917359	0.0
27	7.058149268563992E-5	0.0	0.0	1.5741084498751414	0.0
28	7.058149268563992E-5	0.0	0.0	1.6201275831061785	0.0
29	7.058149268563992E-5	0.0	0.0	1.6749694029229207	7.058149268563992E-5
30	7.058149268563992E-5	0.0	0.0	1.7415277505254791	7.058149268563992E-5
31	7.058149268563992E-5	0.0	0.0	1.7993339930350183	7.058149268563992E-5
32	7.058149268563992E-5	0.0	0.0	1.8604575657007825	7.058149268563992E-5
33	7.058149268563992E-5	0.0	0.0	1.9196754380640344	7.058149268563992E-5
34	7.058149268563992E-5	0.0	0.0	1.985386807754365	7.058149268563992E-5
35	7.058149268563992E-5	0.0	0.0	2.0594267935816015	7.058149268563992E-5
36	7.058149268563992E-5	0.0	0.0	2.133607942394209	7.058149268563992E-5
37	7.058149268563992E-5	0.0	0.0	2.211035839870356	7.058149268563992E-5
38	7.058149268563992E-5	0.0	0.0	2.291781067502728	7.058149268563992E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11905	0.0	43.13146	1
TAGTACG	130	0.0	40.615383	1
TGCGTTG	45	2.3515895E-8	39.11111	1
AGGGCGA	770	0.0	38.285713	6
CGGTCTA	110	0.0	38.0	31
GTTTTTT	13580	0.0	37.876286	2
TAGGGAT	1880	0.0	37.329784	5
TACGGGA	465	0.0	36.90323	4
TAGGGAC	1100	0.0	36.6	5
GCGCGAC	285	0.0	36.280704	9
GGGCGAT	1380	0.0	36.188404	7
CGTTAGG	225	0.0	36.17778	2
AGGGATT	1825	0.0	36.04384	6
CTACGGG	220	0.0	36.0	3
ATAGGGA	1895	0.0	35.98945	4
ACGGGAT	575	0.0	35.965218	5
AGACGGG	560	0.0	35.75	3
CGGGATT	550	0.0	35.6	6
TAAGGGA	1525	0.0	35.48852	4
CTAGGGA	970	0.0	35.381443	4
>>END_MODULE