FastQCFastQC Report
Thu 26 May 2016
SRR1546544_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546544_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences527463
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23290.44154755878611396No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA5480.1038935432437915No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGCG207.852604E-444.01
TCGATAG351.4440957E-744.01
TCTAGCG254.439555E-544.01
AACGGTA207.852604E-444.029
TCGCTAA207.852604E-444.027
GACGATA254.439555E-544.09
CGGTATT207.852604E-444.031
TGCGTAG351.4440957E-744.01
CGTTTTT17950.042.7743721
GCCCTAC850.041.41176626
ACGGGAT2250.041.0666665
TCGTCCC700.040.85714338
TAGGGTC1400.040.8571435
TGACGGG2650.040.6792453
CGTAAGG650.040.6153832
TACGGGA2400.040.3333324
CGAGGGT603.6379788E-1240.3333324
CGGTCTA557.8216544E-1140.031
TACGTAG557.8216544E-1140.01
TGGGCGA2150.039.9069756
Produced by FastQC (version 0.11.5)