##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546544_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	527463
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.601985352527098	31.0	30.0	31.0	28.0	33.0
2	30.928594043563244	31.0	31.0	33.0	28.0	34.0
3	31.085808862422578	31.0	31.0	34.0	28.0	34.0
4	34.903094624646656	35.0	35.0	37.0	32.0	37.0
5	34.68901136193439	35.0	35.0	37.0	32.0	37.0
6	34.7160976220133	35.0	35.0	37.0	32.0	37.0
7	35.403935441917255	35.0	35.0	37.0	33.0	37.0
8	34.384921785983096	35.0	35.0	37.0	33.0	37.0
9	36.53241838764046	39.0	37.0	39.0	32.0	39.0
10	35.90957849176151	38.0	35.0	39.0	30.0	39.0
11	35.577335282285205	37.0	35.0	39.0	30.0	39.0
12	34.89825447472145	37.0	33.0	39.0	29.0	39.0
13	34.44547958814173	37.0	33.0	39.0	26.0	39.0
14	35.332578778037515	38.0	33.0	40.0	26.0	41.0
15	35.73701283312763	38.0	33.0	40.0	29.0	41.0
16	35.93025671942866	38.0	34.0	40.0	30.0	41.0
17	35.86381604017723	37.0	34.0	40.0	29.0	41.0
18	35.78264257398149	37.0	34.0	40.0	29.0	41.0
19	35.79178065570476	37.0	34.0	40.0	29.0	41.0
20	35.58827633407462	37.0	34.0	40.0	29.0	41.0
21	35.39452056352768	37.0	34.0	40.0	29.0	41.0
22	35.33124787899815	37.0	33.0	40.0	28.0	41.0
23	35.287011600813706	36.0	34.0	40.0	29.0	41.0
24	35.23468186394117	36.0	34.0	40.0	28.0	41.0
25	35.0907570767997	36.0	33.0	40.0	27.0	41.0
26	34.893010505002245	36.0	33.0	40.0	27.0	41.0
27	34.6991542534737	36.0	33.0	40.0	26.0	41.0
28	34.75242623653223	36.0	33.0	40.0	26.0	41.0
29	34.65644794042426	36.0	33.0	40.0	26.0	41.0
30	34.68590213910739	36.0	33.0	40.0	26.0	41.0
31	34.59962689326076	36.0	33.0	40.0	25.0	41.0
32	34.32955297338391	36.0	33.0	40.0	24.0	41.0
33	34.16759469384582	36.0	33.0	40.0	24.0	41.0
34	34.09744569761291	36.0	33.0	40.0	24.0	41.0
35	33.88909553845483	36.0	32.0	40.0	23.0	41.0
36	33.82548159776136	36.0	32.0	40.0	23.0	41.0
37	33.84753432942216	36.0	32.0	40.0	23.0	41.0
38	33.88897609879745	36.0	32.0	40.0	23.0	41.0
39	33.83642264955078	36.0	32.0	40.0	23.0	41.0
40	33.74752731471212	36.0	32.0	40.0	23.0	41.0
41	33.82505502755644	36.0	32.0	40.0	23.0	41.0
42	33.68034155950275	36.0	32.0	40.0	23.0	41.0
43	33.737092459565886	35.0	32.0	40.0	23.0	41.0
44	33.44485205597359	35.0	32.0	40.0	23.0	41.0
45	33.38681575769296	35.0	32.0	39.0	23.0	41.0
46	33.357793816817484	35.0	32.0	39.0	23.0	41.0
47	33.30701110788814	35.0	32.0	39.0	23.0	41.0
48	33.3675328885628	35.0	32.0	39.0	23.0	41.0
49	33.40537061367338	35.0	32.0	39.0	23.0	41.0
50	32.8213182725613	35.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	9.0
15	33.0
16	77.0
17	195.0
18	432.0
19	775.0
20	1404.0
21	2300.0
22	3254.0
23	4777.0
24	6330.0
25	8565.0
26	11069.0
27	13372.0
28	15023.0
29	17437.0
30	20864.0
31	24814.0
32	30317.0
33	37085.0
34	52392.0
35	57464.0
36	42934.0
37	51330.0
38	62170.0
39	63039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.06987788716933	23.107213207371892	29.194654411778647	13.628254493680124
2	31.84223348367563	25.089532346344672	30.18922654290443	12.879007627075264
3	29.017390793287873	25.729198066973417	31.923755789505613	13.329655350233097
4	26.195202317508524	27.763463977568094	31.284848415907845	14.756485289015533
5	23.259640960598187	31.863846374058465	30.80197094393351	14.074541721409842
6	20.094679626817427	41.66055249372942	27.653503658076488	10.591264221376665
7	88.26078796048253	3.6144715363921263	6.7265381647622675	1.39820233836307
8	87.08136874055621	5.727984711723855	5.873776928429103	1.3168696192908318
9	85.60638376530676	4.512733594583886	7.357482894534782	2.523399745574571
10	47.280283166781366	28.33032838322309	13.527394338560239	10.861994111435305
11	39.479546432640774	23.578146713608348	24.06348881343336	12.878818040317519
12	36.2891804733223	22.568976402136265	25.86115045036334	15.280692674178095
13	21.980499106098435	34.89780325823802	26.543283604726774	16.578414030936766
14	16.611212540026504	37.098526342132054	29.22764250762613	17.062618610215313
15	15.829735924605137	25.030381277928498	43.16037333424335	15.979509463223012
16	18.79278736138838	21.469183620462477	41.74055810549745	17.997470912651693
17	19.35623920540398	21.45231039902325	29.470882317811864	29.720568077760905
18	21.666164261758645	24.40474497737282	33.82265675507097	20.106434005797563
19	27.433772605851026	25.184894485489977	27.100668672494564	20.280664236164434
20	29.841714015959415	25.030191691170756	26.561294346712472	18.56679994615736
21	23.739295457690872	27.9219585070422	29.19655027935609	19.14219575591084
22	22.563478386161684	24.2839782126898	28.72618553339286	24.42635786775565
23	20.83046583362245	29.062322854873234	28.29373813897847	21.813473172525846
24	20.174495651827712	24.568358349306017	36.944013134570575	18.313132864295696
25	19.167410794690813	25.982106801804107	34.439761651528165	20.410720751976914
26	18.108568752689763	33.36309845429916	28.350803753059456	20.177529039951615
27	18.25075882099787	32.64020414702074	29.847780792207224	19.261256239774163
28	17.14698471741146	28.89662402860485	36.45772310095685	17.49866815302685
29	18.326403937337783	25.704551788466677	36.16196775887598	19.807076515319558
30	19.853714857724615	28.921649480627075	31.596908219154706	19.627727442493597
31	27.45538549623386	26.425929401683153	26.945776291417594	19.172908810665394
32	28.376208378597173	27.104839581164935	27.433962192608767	17.084989847629124
33	27.349975258928115	26.974972652110196	26.614189052123088	19.0608630368386
34	20.46664884551144	27.806310584818274	28.85624204920535	22.870798520464945
35	20.245021925708535	26.353317673467142	31.742700435859955	21.658959964964367
36	27.16816155825148	26.173779013883436	27.126073298032278	19.531986129832806
37	21.567010387458456	32.14234932118462	27.34371889592256	18.946921395434373
38	20.962607803770123	32.15808502207737	25.605208327408747	21.27409884674375
39	21.236750255468156	30.335966693398397	27.193945357304685	21.233337693828762
40	23.4306482160834	26.33170478308431	27.321726832024236	22.91592016880805
41	18.72681116969342	26.07424596606776	28.41090275526435	26.78804010897447
42	21.419891063449	27.056305371182432	25.894896893241803	25.628906672126767
43	21.346710574959758	26.84965580524132	26.796950686588445	25.006682933210485
44	20.30531051467117	29.222144491651548	28.932835099333982	21.5397098943433
45	19.067877746875137	33.55533942665173	25.24916439636524	22.127618430107894
46	21.315428759931976	31.718622917626448	26.728509867042803	20.237438455398767
47	21.322633056726254	27.672841507366392	27.98926180604137	23.01526362986598
48	21.62900525724079	25.270398113232588	31.175267269931727	21.92532935959489
49	21.19162860712505	25.15304391018896	31.83199579875745	21.82333168392854
50	19.50354811617118	31.617194002233333	27.50998648246417	21.36927139913131
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	173.0
1	413.5
2	654.0
3	2525.5
4	4397.0
5	3355.0
6	2313.0
7	2261.0
8	2209.0
9	2351.5
10	2494.0
11	2482.0
12	2470.0
13	2358.0
14	2246.0
15	2171.0
16	2096.0
17	2005.0
18	1914.0
19	1876.5
20	1839.0
21	2063.0
22	2287.0
23	2611.0
24	2935.0
25	3743.5
26	4552.0
27	5145.0
28	5738.0
29	7159.0
30	8580.0
31	10043.0
32	11506.0
33	13345.5
34	15185.0
35	16500.0
36	17815.0
37	19591.5
38	21368.0
39	24734.0
40	28100.0
41	34806.0
42	41512.0
43	46438.5
44	51365.0
45	50363.5
46	49362.0
47	45974.0
48	42586.0
49	40891.5
50	39197.0
51	36663.0
52	34129.0
53	31403.0
54	28677.0
55	25780.0
56	22883.0
57	21493.5
58	20104.0
59	19357.5
60	18611.0
61	16519.0
62	14427.0
63	12102.0
64	9777.0
65	7716.0
66	5655.0
67	4429.0
68	3203.0
69	2637.0
70	2071.0
71	1672.5
72	1274.0
73	1083.0
74	892.0
75	668.5
76	445.0
77	322.0
78	199.0
79	151.0
80	103.0
81	88.0
82	73.0
83	42.5
84	12.0
85	19.0
86	26.0
87	15.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	527463.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.407691418396748
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.92052811233087	22.477526083246794
2	5.982395054092696	3.638216454955873
3	2.670877800541535	2.4364568392533967
4	1.804478670969318	2.1948012239165484
5	1.419510684028162	2.1582021422522817
6	1.293877375290024	2.3606294376638504
7	1.1429307512380071	2.432771991736919
8	1.0293890463907966	2.5041075577703236
9	0.9602970262718431	2.628037408039042
>10	9.661121322482888	54.109034620539596
>50	0.0998078136072601	1.9331724142646638
>100	0.013554147526911867	0.5880635922450606
>500	6.160976148596304E-4	0.10266290173631513
>1k	6.160976148596304E-4	0.4363173323793394
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2329	0.44154755878611396	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	548	0.1038935432437915	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.008341817340742384	0.0
2	0.0	0.0	0.0	0.0386756985798056	0.0
3	0.0	0.0	0.0	0.062374043297823735	0.0
4	0.0	0.0	0.0	0.10825403867190685	0.0
5	0.0	0.0	0.0	0.2178351846480227	0.0
6	0.0	0.0	0.0	0.32931219820158003	0.0
7	0.0	0.0	0.0	0.40344062047954077	0.0
8	0.0	0.0	0.0	0.5734999421760388	0.0
9	0.0	0.0	0.0	0.6332197708654446	0.0
10	0.0	0.0	0.0	0.7642242204666488	0.0
11	0.0	0.0	0.0	0.8705823915611143	0.0
12	0.0	0.0	0.0	0.9811114713259508	0.0
13	0.0	0.0	0.0	1.027181053457778	0.0
14	0.0	0.0	0.0	1.0450022086857278	0.0
15	0.0	0.0	0.0	1.079317411837418	0.0
16	0.0	0.0	0.0	1.14984368571824	0.0
17	0.0	0.0	0.0	1.2279534299088277	0.0
18	0.0	0.0	0.0	1.3477722608031275	0.0
19	0.0	0.0	0.0	1.4046482881263709	0.0
20	0.0	0.0	0.0	1.4615243154496145	0.0
21	0.0	0.0	0.0	1.5347048039388544	0.0
22	0.0	0.0	0.0	1.616606283284325	0.0
23	0.0	0.0	0.0	1.7091246210634679	0.0
24	0.0	0.0	0.0	1.7857176711921026	0.0
25	0.0	0.0	0.0	1.8456270866392523	0.0
26	0.0	0.0	0.0	1.9138783194271447	0.0
27	0.0	0.0	0.0	1.9785274038178982	0.0
28	0.0	0.0	0.0	2.0482953306677434	0.0
29	0.0	0.0	0.0	2.133798958410353	0.0
30	0.0	0.0	0.0	2.2266964697049842	0.0
31	0.0	0.0	0.0	2.3235753029122423	0.0
32	0.0	0.0	0.0	2.4075622365928986	0.0
33	0.0	0.0	0.0	2.4979951200368555	0.0
34	0.0	0.0	0.0	2.585963375630139	0.0
35	0.0	0.0	0.0	2.6959236951217433	0.0
36	0.0	0.0	0.0	2.809486163010486	0.0
37	0.0	0.0	0.0	2.9122421857078127	0.0
38	0.0	0.0	0.0	3.027700521173997	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	20	7.852604E-4	44.0	1
TCGATAG	35	1.4440957E-7	44.0	1
TCTAGCG	25	4.439555E-5	44.0	1
AACGGTA	20	7.852604E-4	44.0	29
TCGCTAA	20	7.852604E-4	44.0	27
GACGATA	25	4.439555E-5	44.0	9
CGGTATT	20	7.852604E-4	44.0	31
TGCGTAG	35	1.4440957E-7	44.0	1
CGTTTTT	1795	0.0	42.774372	1
GCCCTAC	85	0.0	41.411766	26
ACGGGAT	225	0.0	41.066666	5
TCGTCCC	70	0.0	40.857143	38
TAGGGTC	140	0.0	40.857143	5
TGACGGG	265	0.0	40.679245	3
CGTAAGG	65	0.0	40.615383	2
TACGGGA	240	0.0	40.333332	4
CGAGGGT	60	3.6379788E-12	40.333332	4
CGGTCTA	55	7.8216544E-11	40.0	31
TACGTAG	55	7.8216544E-11	40.0	1
TGGGCGA	215	0.0	39.906975	6
>>END_MODULE