FastQCFastQC Report
Thu 26 May 2016
SRR1546543_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546543_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1177699
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30180.2562624235904081No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15530.131867310747483No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG207.856559E-444.0000041
TCGTTGC207.856559E-444.00000425
AAGTACG502.7284841E-1144.01
TACGGTG302.5277695E-644.041
CGATACG351.446133E-744.010
GCGATAC650.043.9999969
CGTTTTT26000.041.3769231
TATAGCG750.041.0666661
ACGGGTA700.040.8571435
ATAACGG700.040.8571432
TACGGGT650.040.6153834
TTGTACG603.6379788E-1240.3333321
ACGTTAG557.8216544E-1140.01
ACGGGCT2600.039.769235
TAAACGG1000.039.62
TGCGTAG501.3478711E-939.61
CGTTAGG1900.039.3684232
TCGTTAG452.3510438E-839.111111
ACCGCAT452.3510438E-839.1111134
TAGTAGG5950.038.823532
Produced by FastQC (version 0.11.5)