##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546543_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1177699
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.703875098815573	31.0	30.0	31.0	28.0	33.0
2	31.0165984687089	31.0	31.0	33.0	28.0	34.0
3	31.18776699309416	31.0	31.0	34.0	28.0	34.0
4	35.01215081272889	35.0	35.0	37.0	32.0	37.0
5	34.80362894084142	35.0	35.0	37.0	32.0	37.0
6	34.842764577366545	35.0	35.0	37.0	32.0	37.0
7	35.432475530674644	35.0	35.0	37.0	35.0	37.0
8	34.4047698096033	36.0	35.0	37.0	33.0	37.0
9	36.54781230178509	39.0	37.0	39.0	32.0	39.0
10	36.0469355922014	38.0	35.0	39.0	31.0	39.0
11	35.738225981341586	37.0	35.0	39.0	30.0	39.0
12	34.78766221250082	37.0	33.0	39.0	30.0	39.0
13	34.184769622798356	37.0	33.0	39.0	25.0	39.0
14	35.04773970258954	37.0	33.0	40.0	25.0	41.0
15	35.55092854795665	37.0	33.0	40.0	29.0	41.0
16	35.80372998533581	37.0	34.0	40.0	30.0	41.0
17	35.76750680776667	37.0	34.0	40.0	30.0	41.0
18	35.72053894925613	37.0	34.0	40.0	30.0	41.0
19	35.693421663769776	37.0	34.0	40.0	29.0	41.0
20	35.50173091766232	36.0	34.0	40.0	29.0	41.0
21	35.255353023140884	36.0	34.0	40.0	29.0	41.0
22	35.20579367053891	36.0	33.0	40.0	28.0	41.0
23	35.22534026096651	36.0	34.0	40.0	29.0	41.0
24	35.16090783808087	36.0	34.0	40.0	29.0	41.0
25	34.986415034741476	36.0	33.0	40.0	27.0	41.0
26	34.799674619745794	35.0	33.0	40.0	27.0	41.0
27	34.60346913769987	35.0	33.0	39.0	27.0	41.0
28	34.65721122290161	36.0	33.0	39.0	26.0	41.0
29	34.537726532840736	36.0	33.0	39.0	26.0	41.0
30	34.60979078694981	36.0	33.0	39.0	27.0	41.0
31	34.50500170247236	35.0	33.0	39.0	26.0	41.0
32	34.272615498527216	35.0	33.0	39.0	25.0	41.0
33	34.16715901091875	35.0	33.0	39.0	25.0	41.0
34	34.10263828023969	35.0	33.0	39.0	24.0	41.0
35	33.90570001333108	35.0	32.0	39.0	24.0	41.0
36	33.82124210006122	35.0	32.0	39.0	23.0	41.0
37	33.817310705027346	35.0	32.0	39.0	23.0	41.0
38	33.87537053185916	35.0	32.0	39.0	23.0	41.0
39	33.81579928317847	35.0	32.0	39.0	23.0	41.0
40	33.684391342779435	35.0	32.0	39.0	23.0	41.0
41	33.7525904326997	35.0	32.0	39.0	23.0	41.0
42	33.60377481852324	35.0	32.0	39.0	23.0	41.0
43	33.6444422556188	35.0	32.0	39.0	23.0	41.0
44	33.36158899684894	35.0	32.0	39.0	23.0	41.0
45	33.27661906819994	35.0	32.0	39.0	23.0	41.0
46	33.24481807320886	35.0	32.0	39.0	23.0	40.0
47	33.199013500053915	35.0	32.0	39.0	23.0	40.0
48	33.25857371026043	35.0	32.0	39.0	23.0	40.0
49	33.2725730428573	35.0	32.0	39.0	24.0	40.0
50	32.65032066767485	35.0	31.0	38.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	6.0
13	4.0
14	24.0
15	45.0
16	149.0
17	368.0
18	751.0
19	1497.0
20	2708.0
21	4272.0
22	6684.0
23	9365.0
24	13231.0
25	17633.0
26	22797.0
27	28062.0
28	33040.0
29	39399.0
30	47969.0
31	57552.0
32	70640.0
33	89319.0
34	131560.0
35	144620.0
36	95493.0
37	111488.0
38	126758.0
39	122261.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.95290477447973	24.20380759430041	27.91698048482677	11.926307146393093
2	31.69213865342503	25.36471543238128	29.451328395455885	13.49181751873781
3	30.75548166382072	25.590239950955212	29.652992827539126	14.001285557684943
4	27.681945896192488	27.817124749193127	28.413457088780746	16.08747226583363
5	24.090620778314324	32.144970828709205	27.94822785788219	15.816180535094283
6	20.910860924565615	41.43749803642527	26.789527714636762	10.862113324372356
7	88.32069994115645	4.365291980378688	5.657048193129144	1.6569598853357268
8	87.00593275531354	6.095445440643153	5.1357774779464025	1.762844326096906
9	85.57594088132876	4.565512919684911	6.903461750413306	2.955084448573022
10	55.248582192903285	20.758614892260248	12.32505079820905	11.667752116627424
11	47.55603936150069	21.50693853013376	17.602884947681876	13.334137160683673
12	40.854666599869745	22.40572506217633	22.150311751984166	14.58929658596976
13	22.86271789311191	38.17732714386274	23.30909680656942	15.650858156455937
14	16.889629693155893	40.19651880488988	27.830370918205755	15.083480583748479
15	15.928008769643176	22.558480562520643	45.673385134911385	15.840125532924795
16	17.873242653683157	18.79147388254554	43.28644246110424	20.048841002667064
17	19.66181511574689	18.5049830219776	27.324808800890548	34.50839306138496
18	23.833594152665494	22.120507871705758	32.53700648467902	21.508891490949726
19	31.62726638979909	23.56671781159702	24.185466744898314	20.620549053705574
20	33.04486120816949	23.650185658644528	23.05274947163919	20.252203661546798
21	24.894985900472022	28.13053250448544	26.512546924129172	20.461934670913365
22	24.091045335013444	23.462361774952683	25.253311754531506	27.193281135502367
23	21.141734857548492	29.26087226022948	25.13596428289402	24.461428599328013
24	21.75513437644084	23.658761703966803	36.07729988732265	18.508804032269705
25	20.2421841234475	24.14861522341447	34.2069578050079	21.402242848130122
26	19.00044068985369	33.23676083617291	25.741806692541978	22.020991781431416
27	19.9807421081278	31.464576262695303	28.75998026660462	19.79470136257227
28	18.170092697709688	27.010382109520343	36.460165118591426	18.359360074178547
29	18.780265585688703	25.690944799987093	35.499138574457476	20.02965103986672
30	22.38712947875476	28.43808137732986	29.59788536799301	19.57690377592237
31	32.406243021349255	23.682112322418547	23.718114730504144	20.19352992572805
32	31.895076755605633	25.027787235957575	25.043750567844587	18.033385440592205
33	30.28057254018217	25.186316707409954	24.28353934239564	20.249571410012233
34	21.793769036060997	25.995946332636777	27.755479116480526	24.454805514821697
35	21.62335197703318	25.018446988576876	30.81483469035806	22.543366344031877
36	31.77747454994867	24.079921949496434	24.670480317975986	19.47212318257891
37	22.444274810456662	32.101835868078346	25.245245177248176	20.208644144216816
38	22.802685575855968	33.4299341342737	21.28047998682176	22.48690030304857
39	22.881143653853826	29.97225946527933	24.32922164322123	22.817375237645614
40	25.928102172116986	24.229280996247766	24.38364981204875	25.4589670195865
41	19.771011098761228	23.1216974795767	25.95247172664662	31.15481969501545
42	22.892691596069962	25.52952834298068	23.067524044768657	28.51025601618071
43	22.032539723647552	25.732636267840935	24.912902193174997	27.321921815336513
44	20.698242929645012	28.602724465249608	28.79369006851496	21.905342536590418
45	19.79393716051385	34.64068492883156	23.33635334665309	22.2290245640015
46	23.039503302626564	30.527409805052052	25.84998373947842	20.58310315284296
47	22.254922522647977	26.65052785134402	26.33261979504101	24.761929830967
48	23.4326428060141	24.04663670428522	30.671928905433393	21.84879158426729
49	22.92062742687223	23.0458716531134	31.405732704196915	22.627768215817454
50	20.37456090223393	32.26469581786178	25.54481238414909	21.815930895755198
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	891.5
2	1448.0
3	3691.0
4	5934.0
5	4529.0
6	3124.0
7	3318.0
8	3512.0
9	3704.5
10	3897.0
11	3848.5
12	3800.0
13	3651.5
14	3503.0
15	3352.0
16	3201.0
17	3030.0
18	2859.0
19	2973.0
20	3087.0
21	3457.0
22	3827.0
23	4340.0
24	4853.0
25	5535.0
26	6217.0
27	7822.0
28	9427.0
29	11278.0
30	13129.0
31	15210.0
32	17291.0
33	18621.5
34	19952.0
35	24017.0
36	28082.0
37	29842.5
38	31603.0
39	42846.0
40	54089.0
41	72282.5
42	90476.0
43	103056.0
44	115636.0
45	112588.0
46	109540.0
47	101964.5
48	94389.0
49	92382.0
50	90375.0
51	83815.0
52	77255.0
53	71038.5
54	64822.0
55	61881.0
56	58940.0
57	56588.0
58	54236.0
59	51612.5
60	48989.0
61	45915.0
62	42841.0
63	38250.0
64	33659.0
65	28399.5
66	23140.0
67	20155.0
68	17170.0
69	15048.5
70	12927.0
71	10591.5
72	8256.0
73	6850.5
74	5445.0
75	4194.5
76	2944.0
77	2270.5
78	1597.0
79	1222.5
80	848.0
81	731.0
82	614.0
83	456.0
84	298.0
85	179.5
86	61.0
87	52.0
88	43.0
89	29.5
90	16.0
91	13.0
92	10.0
93	5.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1177699.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.127112579548864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.1899297169455	17.301173774338665
2	6.541652680956661	2.894957706556714
3	2.428190923126455	1.6118656176197326
4	1.3269186277856537	1.1744351144365461
5	0.8567985410090443	0.947923888745017
6	0.5984072671701421	0.7944614981456344
7	0.5171358701220293	0.8009906531979172
8	0.42528940618421823	0.7528341255622145
9	0.388378070158565	0.7734316753643846
>10	6.67522922735919	37.81589487627792
>50	1.8169247400560022	27.08815659102774
>100	0.23055673538748866	7.077370954831568
>500	0.0038234947825454128	0.5797836702782975
>1k	7.646989565090825E-4	0.38671985361769995
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3018	0.2562624235904081	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1553	0.131867310747483	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.009510070060346489	0.0
2	0.0	0.0	0.0	0.04254058125208564	0.0
3	0.0	0.0	0.0	0.06427788424716332	0.0
4	0.0	0.0	0.0	0.10469568200363591	0.0
5	0.0	0.0	0.0	0.18960702182815814	0.0
6	0.0	0.0	0.0	0.2861512152086399	0.0
7	0.0	0.0	0.0	0.3526367942912408	0.0
8	0.0	0.0	0.0	0.5373189584095767	0.0
9	0.0	0.0	0.0	0.6225699435933969	0.0
10	0.0	0.0	0.0	0.7588526440117551	0.0
11	0.0	0.0	0.0	0.8544628126541671	0.0
12	0.0	0.0	0.0	0.9523655874718413	0.0
13	0.0	0.0	0.0	0.9945665233646288	0.0
14	0.0	0.0	0.0	1.009850564533043	0.0
15	0.0	0.0	0.0	1.036512725237943	0.0
16	0.0	0.0	0.0	1.0934882342601973	0.0
17	0.0	0.0	0.0	1.1547093102736778	0.0
18	0.0	0.0	0.0	1.2614428644331022	0.0
19	0.0	0.0	0.0	1.3029645096072935	0.0
20	0.0	0.0	0.0	1.3522130867055164	0.0
21	0.0	0.0	0.0	1.410971733864086	0.0
22	0.0	0.0	0.0	1.4694756470031816	0.0
23	0.0	0.0	0.0	1.5402067930770087	0.0
24	0.0	0.0	0.0	1.5948896959240009	0.0
25	0.0	0.0	0.0	1.6397228833513486	0.0
26	0.0	0.0	0.0	1.686933588293783	0.0
27	0.0	0.0	0.0	1.7356726973530587	0.0
28	0.0	0.0	0.0	1.7869591466070702	0.0
29	0.0	0.0	0.0	1.8436799216098512	0.0
30	0.0	0.0	0.0	1.9263835665989357	0.0
31	0.0	0.0	0.0	2.00475673325697	0.0
32	0.0	0.0	0.0	2.067251479367818	0.0
33	0.0	0.0	0.0	2.134840905868138	0.0
34	0.0	0.0	0.0	2.205147495242842	0.0
35	0.0	0.0	0.0	2.3005029298657806	0.0
36	0.0	0.0	0.0	2.3750550862317112	0.0
37	0.0	0.0	0.0	2.4570794405022	0.0
38	0.0	0.0	0.0	2.546236347317948	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	20	7.856559E-4	44.000004	1
TCGTTGC	20	7.856559E-4	44.000004	25
AAGTACG	50	2.7284841E-11	44.0	1
TACGGTG	30	2.5277695E-6	44.0	41
CGATACG	35	1.446133E-7	44.0	10
GCGATAC	65	0.0	43.999996	9
CGTTTTT	2600	0.0	41.376923	1
TATAGCG	75	0.0	41.066666	1
ACGGGTA	70	0.0	40.857143	5
ATAACGG	70	0.0	40.857143	2
TACGGGT	65	0.0	40.615383	4
TTGTACG	60	3.6379788E-12	40.333332	1
ACGTTAG	55	7.8216544E-11	40.0	1
ACGGGCT	260	0.0	39.76923	5
TAAACGG	100	0.0	39.6	2
TGCGTAG	50	1.3478711E-9	39.6	1
CGTTAGG	190	0.0	39.368423	2
TCGTTAG	45	2.3510438E-8	39.11111	1
ACCGCAT	45	2.3510438E-8	39.11111	34
TAGTAGG	595	0.0	38.82353	2
>>END_MODULE