FastQCFastQC Report
Thu 26 May 2016
SRR1546542_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546542_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1136013
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43680.38450264213525726No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA17230.15167079954190665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGT254.4428078E-544.020
TATTACG254.4428078E-544.01
TTACGCT351.4460602E-744.040
TATCGCG207.8564446E-444.01
CGTTTTT34400.042.401161
ACGGGAC1200.042.1666645
CGGGTAT1150.042.086966
TATACGG1650.041.333332
TACGGGA2400.041.254
AGTACGG1000.039.62
ACGGGCT1350.039.111115
ACGGGAT3150.039.111115
GTTGCGT452.3508619E-839.1111137
ACGATTG850.038.8235321
CGTTAGG1250.038.722
CTACGGG1950.038.3589743
AAGTGCG750.038.133331
GTAGGGC4250.037.7882354
CTTAACG357.290133E-637.7142871
GACCGTT357.290133E-637.71428718
Produced by FastQC (version 0.11.5)