##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546542_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1136013
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.648132547778943	31.0	30.0	31.0	28.0	33.0
2	30.9655470491975	31.0	31.0	33.0	28.0	34.0
3	31.14054064522149	31.0	31.0	34.0	28.0	34.0
4	34.95699609071375	35.0	35.0	37.0	32.0	37.0
5	34.750370814418496	35.0	35.0	37.0	32.0	37.0
6	34.79486414328005	35.0	35.0	37.0	32.0	37.0
7	35.40703407443401	35.0	35.0	37.0	33.0	37.0
8	34.361595333856215	35.0	35.0	37.0	33.0	37.0
9	36.50928202406134	39.0	37.0	39.0	32.0	39.0
10	35.99006437426332	38.0	35.0	39.0	30.0	39.0
11	35.67321764803748	37.0	35.0	39.0	30.0	39.0
12	34.793688980671874	37.0	33.0	39.0	29.0	39.0
13	34.23538991191122	37.0	33.0	39.0	25.0	39.0
14	35.112106111461756	37.0	33.0	40.0	25.0	41.0
15	35.62929385491187	37.0	33.0	40.0	29.0	41.0
16	35.85224993023847	37.0	34.0	40.0	30.0	41.0
17	35.80734903561843	37.0	34.0	40.0	30.0	41.0
18	35.73198986279206	37.0	34.0	40.0	29.0	41.0
19	35.69816014429412	37.0	34.0	40.0	29.0	41.0
20	35.4810481922302	36.0	34.0	40.0	29.0	41.0
21	35.25607453435832	36.0	34.0	40.0	29.0	41.0
22	35.219247491005824	36.0	33.0	40.0	28.0	41.0
23	35.23133185975865	36.0	34.0	40.0	29.0	41.0
24	35.15097538496479	36.0	34.0	40.0	29.0	41.0
25	34.97882330571921	36.0	33.0	40.0	27.0	41.0
26	34.80275489805134	36.0	33.0	40.0	27.0	41.0
27	34.624533346009244	35.0	33.0	40.0	27.0	41.0
28	34.67107946828073	36.0	33.0	40.0	26.0	41.0
29	34.59303810783855	36.0	33.0	39.0	26.0	41.0
30	34.63786329909957	36.0	33.0	39.0	27.0	41.0
31	34.51241579101647	36.0	33.0	40.0	26.0	41.0
32	34.26866946064878	35.0	33.0	40.0	25.0	41.0
33	34.11043359539019	35.0	33.0	40.0	24.0	41.0
34	34.08224641795472	35.0	33.0	40.0	24.0	41.0
35	33.875182766394396	35.0	32.0	39.0	23.0	41.0
36	33.742217738705456	35.0	32.0	40.0	23.0	41.0
37	33.75475104598275	35.0	32.0	40.0	23.0	41.0
38	33.82527752763393	35.0	32.0	40.0	23.0	41.0
39	33.77811345468758	35.0	32.0	40.0	23.0	41.0
40	33.66651878103507	35.0	32.0	40.0	23.0	41.0
41	33.752892792600086	35.0	32.0	40.0	23.0	41.0
42	33.59730830545073	35.0	32.0	39.0	23.0	41.0
43	33.617969160564186	35.0	32.0	39.0	23.0	41.0
44	33.25568809511863	35.0	32.0	39.0	23.0	41.0
45	33.231062496643965	35.0	31.0	39.0	23.0	41.0
46	33.23384063386599	35.0	32.0	39.0	23.0	41.0
47	33.18331744443066	35.0	31.0	39.0	23.0	41.0
48	33.26386141707886	35.0	32.0	39.0	23.0	41.0
49	33.290979944771756	35.0	32.0	39.0	23.0	41.0
50	32.66660064629542	35.0	31.0	38.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	7.0
14	12.0
15	64.0
16	153.0
17	415.0
18	899.0
19	1727.0
20	2960.0
21	4455.0
22	6918.0
23	9711.0
24	13460.0
25	18400.0
26	23592.0
27	28299.0
28	32778.0
29	38217.0
30	45198.0
31	54889.0
32	66107.0
33	82970.0
34	121067.0
35	135005.0
36	89101.0
37	105539.0
38	125517.0
39	128549.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58286744958024	23.25686413799842	28.057425399181174	13.102843013240165
2	31.806238132838267	25.25243989285334	29.50890526780944	13.432416706498957
3	29.86400683794992	25.718455686686685	31.215311796607963	13.202225678755436
4	27.34062022177563	27.219670901653416	30.00141723730274	15.438291639268211
5	22.76408808702013	32.39927712094844	29.905731712577232	14.930903079454197
6	20.441139317947947	41.87733767131186	27.634630941723376	10.046892069016817
7	88.18305776430375	4.035077063378676	6.248343989021253	1.5335211832963178
8	86.915290582062	5.768595957968791	5.7939477805271595	1.5221656794420486
9	85.45342350835774	4.777322090504247	7.235040444079425	2.53421395705859
10	51.51701609048488	24.900419273370993	12.96534458672568	10.617220049418448
11	44.85943382690163	21.358118260970606	21.313224408523492	12.469223503604272
12	39.54514605026527	22.724739945757662	23.74946413465339	13.980649869323678
13	21.07114971395574	39.69259154604745	23.925166349328748	15.311092390668065
14	15.558272660612158	40.47946634413515	28.479955775153982	15.482305220098713
15	15.08142952589451	23.01857461138209	47.09435543431281	14.805640428410591
16	17.839760636542014	19.670109408959227	43.54633265640446	18.9437972980943
17	18.465281647305094	19.544230567783995	28.029784870419615	33.9607029144913
18	23.22174130049568	23.86803672141076	33.29521757233412	19.615004405759443
19	30.271924705087	24.816529388308055	25.168109871982097	19.743436034622842
20	31.579568191561187	24.42700919795812	24.789064913869822	19.204357696610867
21	22.66039209058347	28.587612993865385	28.229694554551756	20.52230036099939
22	22.75775013138054	24.519173636217193	26.801805965248636	25.921270267153634
23	20.47767059003726	29.394822066296776	26.58235425122776	23.545153092438202
24	20.00214786274453	23.711436400815835	38.19692204226536	18.089493694174273
25	18.24274898262608	25.81106026075406	34.96289215000181	20.98329860661806
26	17.642051631451398	34.90197735413239	26.841330160834424	20.614640853581783
27	18.330424035640437	32.91766907597008	29.32871366788936	19.423193220500117
28	16.583789093962835	27.506023258536654	37.906608463107375	18.003579184393136
29	17.304819575128104	25.499003972665808	36.882412437181614	20.31376401502448
30	20.370981670104126	29.59878099986532	31.021299932307112	19.00893739772344
31	29.515067169125704	25.861763905870795	24.991087249881826	19.632081675121675
32	30.57966766225387	25.910266871946007	25.86827791583371	17.641787549966416
33	27.88938154757032	27.033581481902058	25.63474185594707	19.442295114580553
34	19.84334686310808	27.568434516154305	28.7153404054355	23.872878215302112
35	20.35900997611823	26.39899367348789	31.357915798498787	21.884080551895092
36	29.336284003792212	25.483071056405166	26.472760434959813	18.707884504842813
37	21.048086597600555	32.519962359585676	27.40576032140477	19.026190721408998
38	20.7428084009602	33.074621505211645	24.243560593056593	21.939009500771558
39	21.624312397833474	31.306595963250423	25.970741532007118	21.09835010690899
40	24.843817808422965	25.235802759299407	25.9658120109541	23.954567421323524
41	18.41228929598517	24.82295537110931	27.30919452506265	29.45556080784287
42	22.30194548829987	26.415806861365144	24.11416066541492	27.168086984920066
43	21.674047744171943	26.8915056429812	26.276283810132455	25.158162802714408
44	20.191934423285648	30.30079761411181	28.612084544807146	20.895183417795398
45	18.38508890303192	36.33937287689489	23.646560382671677	21.628977837401507
46	21.809785627453206	32.23466632864236	25.925055435105055	20.03049260879937
47	20.962260114981078	27.71896096259462	27.503646525171803	23.815132397252498
48	23.16971724795403	24.26433500320859	30.829840855694435	21.73610689314295
49	20.6407849205951	25.244253366818864	31.71838702550059	22.396574687085447
50	19.49792828075031	33.11484991809073	26.60876239972606	20.778459401432904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	424.0
1	929.0
2	1434.0
3	4806.5
4	8179.0
5	6189.0
6	4199.0
7	4229.0
8	4259.0
9	4573.5
10	4888.0
11	4855.5
12	4823.0
13	4572.5
14	4322.0
15	4228.5
16	4135.0
17	3944.5
18	3754.0
19	3651.0
20	3548.0
21	4164.0
22	4780.0
23	5801.0
24	6822.0
25	7382.0
26	7942.0
27	9853.0
28	11764.0
29	14260.0
30	16756.0
31	18932.5
32	21109.0
33	24500.0
34	27891.0
35	31616.5
36	35342.0
37	38047.5
38	40753.0
39	50956.0
40	61159.0
41	77535.5
42	93912.0
43	104697.0
44	115482.0
45	112671.0
46	109860.0
47	100944.5
48	92029.0
49	85817.0
50	79605.0
51	76350.5
52	73096.0
53	66794.0
54	60492.0
55	55349.5
56	50207.0
57	46687.5
58	43168.0
59	40516.5
60	37865.0
61	35234.5
62	32604.0
63	28187.0
64	23770.0
65	19442.5
66	15115.0
67	12831.0
68	10547.0
69	8858.5
70	7170.0
71	6198.5
72	5227.0
73	4485.5
74	3744.0
75	2734.5
76	1725.0
77	1360.0
78	995.0
79	800.0
80	605.0
81	453.5
82	302.0
83	215.5
84	129.0
85	97.5
86	66.0
87	37.0
88	8.0
89	4.5
90	1.0
91	2.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1136013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.324749362064164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22279026446392	19.809725578488617
2	7.557395039243548	3.827782703978998
3	2.80540947447394	2.13138875397038
4	1.3898211627879244	1.4078749042278704
5	0.8310953768461335	1.0523641057299298
6	0.5905288069337996	0.8972996416006351
7	0.4411330197230589	0.7820108211871882
8	0.361799615996942	0.7329987675530892
9	0.3265969994176447	0.744388843738866
>10	5.843508598787653	37.89488237749233
>50	1.4768561840885093	24.962077116207475
>100	0.15237128282758458	5.221813792217897
>500	0.0	0.0
>1k	6.941744092372506E-4	0.5353925936067789
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4368	0.38450264213525726	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1723	0.15167079954190665	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012147748309218293	0.0
2	0.0	0.0	0.0	0.042957254890569035	0.0
3	0.0	0.0	0.0	0.06505207246748057	0.0
4	0.0	0.0	0.0	0.09471722594723828	0.0
5	0.0	0.0	0.0	0.15959324409139683	0.0
6	0.0	0.0	0.0	0.24436340077094187	0.0
7	0.0	0.0	0.0	0.3069507127119144	0.0
8	0.0	0.0	0.0	0.47596286310103847	0.0
9	0.0	0.0	0.0	0.5630217259837695	0.0
10	0.0	0.0	0.0	0.6968230117084928	0.0
11	0.0	0.0	0.0	0.7981422747803062	0.0
12	0.0	0.0	0.0	0.8981411304272047	0.0
13	0.0	0.0	0.0	0.9445314446225528	0.0
14	0.0	0.0	0.0	0.9664502078761422	0.0
15	0.0	0.0	0.0	0.9922421662428159	0.0
16	0.0	0.0	0.0	1.0442662187844682	0.0
17	0.0	0.0	0.0	1.1028923084506956	0.0
18	0.0	0.0	0.0	1.208788983928881	0.0
19	0.0	0.0	0.0	1.2556194339325342	0.0
20	0.0	0.0	0.0	1.3007773678646284	0.0
21	0.0	0.0	0.0	1.3550901266094666	0.0
22	0.0	0.0	0.0	1.414860569377287	8.802716166100212E-5
23	0.0	0.0	0.0	1.4767436640249716	8.802716166100212E-5
24	0.0	0.0	0.0	1.5254226844235057	8.802716166100212E-5
25	0.0	0.0	0.0	1.5713728628105488	8.802716166100212E-5
26	0.0	0.0	0.0	1.617851204167558	1.7605432332200424E-4
27	0.0	0.0	0.0	1.6683787949609732	1.7605432332200424E-4
28	0.0	0.0	0.0	1.7188183585927275	1.7605432332200424E-4
29	0.0	0.0	0.0	1.7785888013605478	1.7605432332200424E-4
30	0.0	0.0	0.0	1.8591336542803647	1.7605432332200424E-4
31	0.0	0.0	0.0	1.9249779712027943	1.7605432332200424E-4
32	0.0	0.0	0.0	1.9868610658504788	1.7605432332200424E-4
33	0.0	0.0	0.0	2.0507687852163663	1.7605432332200424E-4
34	0.0	0.0	0.0	2.126208062759845	1.7605432332200424E-4
35	0.0	0.0	0.0	2.2139711429358644	1.7605432332200424E-4
36	0.0	0.0	0.0	2.288090013054428	1.7605432332200424E-4
37	0.0	0.0	0.0	2.367754594357635	1.7605432332200424E-4
38	0.0	0.0	0.0	2.454197267108739	1.7605432332200424E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGT	25	4.4428078E-5	44.0	20
TATTACG	25	4.4428078E-5	44.0	1
TTACGCT	35	1.4460602E-7	44.0	40
TATCGCG	20	7.8564446E-4	44.0	1
CGTTTTT	3440	0.0	42.40116	1
ACGGGAC	120	0.0	42.166664	5
CGGGTAT	115	0.0	42.08696	6
TATACGG	165	0.0	41.33333	2
TACGGGA	240	0.0	41.25	4
AGTACGG	100	0.0	39.6	2
ACGGGCT	135	0.0	39.11111	5
ACGGGAT	315	0.0	39.11111	5
GTTGCGT	45	2.3508619E-8	39.11111	37
ACGATTG	85	0.0	38.823532	1
CGTTAGG	125	0.0	38.72	2
CTACGGG	195	0.0	38.358974	3
AAGTGCG	75	0.0	38.13333	1
GTAGGGC	425	0.0	37.788235	4
CTTAACG	35	7.290133E-6	37.714287	1
GACCGTT	35	7.290133E-6	37.714287	18
>>END_MODULE