FastQCFastQC Report
Thu 26 May 2016
SRR1546541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546541_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1960424
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228821.1671964840259046No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT162700.043.026431
GTTTTTT182350.038.46232
TAGGGAC16700.038.0718575
CGTTAGG2200.038.0000042
TTACGGG5150.037.592233
AGTACGG2100.036.6666642
CGTAAGG3850.036.571432
TTAGGGA19400.036.402064
GCGTAAG2000.036.31
AGGGATC18400.036.1086966
TAGCGGG11450.035.930133
ATAGGGA21250.035.9247064
TCGTTAG800.035.751
CGACGGT1600.035.7528
TATGGGA17000.035.7176484
TAGGGAT24350.035.5975345
ACGGGAT9000.035.4444475
GGCGATT7200.035.4444478
GACCGAT4800.035.2916649
TAGTAGG10900.035.1192672
Produced by FastQC (version 0.11.5)