##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546541_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1960424
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.614265587444347	31.0	30.0	31.0	28.0	34.0
2	30.949268117509273	31.0	31.0	33.0	28.0	34.0
3	31.066923787915268	31.0	31.0	34.0	28.0	34.0
4	34.91799121006476	35.0	35.0	37.0	32.0	37.0
5	34.73921661844581	35.0	35.0	37.0	32.0	37.0
6	34.77027520577181	35.0	35.0	37.0	32.0	37.0
7	35.39013703158092	35.0	35.0	37.0	33.0	37.0
8	34.34327267978764	35.0	35.0	37.0	32.0	37.0
9	36.46698724357588	39.0	37.0	39.0	32.0	39.0
10	35.980531762516684	38.0	35.0	39.0	30.0	39.0
11	35.67559874802594	37.0	35.0	39.0	30.0	39.0
12	35.257105095632376	37.0	34.0	39.0	30.0	39.0
13	35.052677890089086	37.0	34.0	39.0	28.0	39.0
14	35.9933514382603	38.0	34.0	40.0	29.0	41.0
15	36.234943053135446	38.0	34.0	40.0	30.0	41.0
16	36.31604438631643	38.0	34.0	40.0	30.0	41.0
17	36.22469782047149	38.0	34.0	40.0	30.0	41.0
18	36.074258935822044	38.0	34.0	40.0	30.0	41.0
19	35.987028826417145	37.0	34.0	40.0	30.0	41.0
20	35.757284138533294	37.0	34.0	40.0	29.0	41.0
21	35.573538173374736	37.0	34.0	40.0	29.0	41.0
22	35.536202372547976	37.0	34.0	40.0	29.0	41.0
23	35.46649500312177	37.0	34.0	40.0	29.0	41.0
24	35.33328708483471	37.0	34.0	40.0	28.0	41.0
25	35.14946511570966	36.0	33.0	40.0	27.0	41.0
26	34.97190097652344	36.0	33.0	40.0	27.0	41.0
27	34.78026692184956	36.0	33.0	40.0	26.0	41.0
28	34.76273704055858	36.0	33.0	40.0	26.0	41.0
29	34.56696714588273	36.0	33.0	40.0	25.0	41.0
30	34.528339277625655	36.0	33.0	40.0	25.0	41.0
31	34.397935854692655	36.0	33.0	40.0	24.0	41.0
32	34.053038016265866	36.0	33.0	40.0	23.0	41.0
33	33.7828587081162	36.0	32.0	40.0	23.0	41.0
34	33.55037277650141	36.0	32.0	40.0	21.0	41.0
35	33.28816521323959	36.0	31.0	40.0	20.0	41.0
36	33.180186020983214	36.0	31.0	40.0	18.0	41.0
37	33.18157500622314	36.0	31.0	40.0	18.0	41.0
38	33.1865897377302	36.0	31.0	40.0	18.0	41.0
39	33.080465246293656	36.0	31.0	40.0	18.0	41.0
40	32.9921210921719	36.0	31.0	40.0	18.0	41.0
41	32.9908790139276	35.0	31.0	40.0	18.0	41.0
42	32.82469353568412	35.0	31.0	40.0	18.0	41.0
43	32.85192641999894	35.0	31.0	40.0	18.0	41.0
44	32.54195571978307	35.0	31.0	39.0	17.0	41.0
45	32.47824603249093	35.0	30.0	39.0	18.0	41.0
46	32.42237903637172	35.0	30.0	39.0	18.0	41.0
47	32.3609867049169	35.0	30.0	39.0	18.0	41.0
48	32.36919003236034	35.0	30.0	39.0	18.0	41.0
49	32.375254536773674	35.0	30.0	39.0	18.0	41.0
50	31.825009283705974	35.0	30.0	38.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	4.0
13	10.0
14	32.0
15	93.0
16	338.0
17	883.0
18	1991.0
19	3769.0
20	6492.0
21	9754.0
22	14360.0
23	20194.0
24	27747.0
25	37965.0
26	50023.0
27	59516.0
28	64373.0
29	68646.0
30	77178.0
31	89281.0
32	106571.0
33	129280.0
34	173993.0
35	193472.0
36	172061.0
37	198199.0
38	230743.0
39	223450.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.438492897454836	22.56032368508037	26.77915593769511	18.222027479769682
2	35.982318110776035	23.501956719566788	27.404326819096276	13.111398350560899
3	27.78271435158925	24.415738636131774	34.85506196618691	12.946485046092072
4	25.267289117048147	26.566191803405793	33.858797892700764	14.307721186845296
5	22.08797688663269	30.60307362080856	32.94924975413482	14.359699738423934
6	19.566991630382	39.82934304007704	30.633730254271523	9.969935075269431
7	83.91791775656695	3.2931651520283363	11.23154990961139	1.5573671817933263
8	82.1467192811351	5.422041354319269	10.772108482654772	1.6591308818908561
9	80.32644978841313	4.5909966415428505	12.417925918066704	2.664627651977327
10	42.4831056955026	28.70567795538108	17.61343464475032	11.197781704365994
11	32.46603795913537	22.777980681730075	29.46148384227086	15.294497516863698
12	30.46667455611643	20.942969480071657	31.53195431192436	17.05840165188755
13	22.411274295764592	27.618362150228727	31.62672972785479	18.343633826151894
14	17.80829045145336	29.95265309953357	33.248470738982995	18.990585710030075
15	17.3661412021073	23.644527918450294	40.61483638233362	18.37449449710879
16	20.35452534757787	21.470865486241753	38.510342660567304	19.66426650561307
17	20.678026794203703	21.01672903412731	32.69568215855346	25.60956201311553
18	22.28701546196129	22.975182919613307	34.84230962281629	19.895491995609113
19	25.7633042647917	24.68792465303424	29.599209150673527	19.949561931500533
20	27.250533558046623	23.594640751184436	29.815335866118758	19.339489824650176
21	24.190532252206665	25.40730984725753	31.42401847763545	18.97813942290035
22	23.33581919013438	22.179436693286757	31.53317853688794	22.951565579690925
23	21.53850391547951	25.771057689561033	31.205035237275204	21.485403157684253
24	20.91802589643873	23.081180397709883	36.49297294870906	19.507820757142333
25	20.703072396583597	24.866304432102442	33.28346316919197	21.14716000212199
26	20.077850505808946	28.068570880585014	30.649798206918504	21.203780406687535
27	19.56056444932321	26.82572749568461	32.57193341848498	21.041774636507206
28	19.308425116199352	25.125738105634294	36.12687867522536	19.438958102940994
29	20.037706128878245	23.912888232341576	35.27104340693646	20.778362231843722
30	21.57686296433833	25.345078411608917	33.4189950745349	19.65906354951786
31	26.088335992622003	24.394212680522173	29.449700676996404	20.067750649859416
32	26.093742986211144	24.86110147600723	29.0961547093894	19.949000828392226
33	25.472193770327234	25.262698273434726	28.456242119051794	20.80886583718624
34	21.304217863074516	26.22794864784353	30.17433983668839	22.293493652393565
35	22.0023831579291	25.74616511530159	30.530895357330863	21.720556369438448
36	25.9099562135538	26.541656294760724	27.16876553235423	20.379621959331246
37	22.446878838455355	29.31319959355731	27.681919829587887	20.558001738399447
38	22.171122165409116	29.375124972965033	27.27435493546294	21.179397926162913
39	22.110216973471044	28.439868110163925	27.383259947848014	22.066654968517017
40	23.913857410437743	25.72045639106642	28.26067218112	22.105014017375833
41	20.744134942236986	25.56248036139121	29.053357845037603	24.640026851334202
42	22.15801275642412	26.564457484707386	26.580984521715713	24.69654523715278
43	21.818749413392204	26.120165841675064	27.567352776746258	24.493731968186474
44	21.262084120578	26.984519675335537	29.43026610569958	22.32313009838688
45	20.866149363607057	29.558656698754966	27.125866649255464	22.449327288382513
46	22.442594051082825	28.650434803899564	28.174619368055076	20.732351776962535
47	21.455562674197008	26.691674862172672	29.314729874761785	22.538032588868532
48	21.767995086777145	24.924965211607287	31.10903559638119	22.19800410523438
49	21.627668300326867	25.25892357979702	30.866179969231144	22.24722815064496
50	20.696696224898286	27.763840883400732	29.702197075734638	21.83726581596634
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	710.0
1	1720.5
2	2731.0
3	21692.5
4	40654.0
5	29684.5
6	18715.0
7	18533.0
8	18351.0
9	18496.5
10	18642.0
11	18197.0
12	17752.0
13	16815.5
14	15879.0
15	14736.5
16	13594.0
17	12654.0
18	11714.0
19	11149.5
20	10585.0
21	10269.0
22	9953.0
23	10669.0
24	11385.0
25	12546.5
26	13708.0
27	16645.0
28	19582.0
29	22365.0
30	25148.0
31	30926.0
32	36704.0
33	40843.5
34	44983.0
35	52483.5
36	59984.0
37	63051.0
38	66118.0
39	75826.0
40	85534.0
41	100622.0
42	115710.0
43	126891.0
44	138072.0
45	138068.5
46	138065.0
47	138329.0
48	138593.0
49	136570.0
50	134547.0
51	127245.5
52	119944.0
53	113273.5
54	106603.0
55	101021.0
56	95439.0
57	94676.5
58	93914.0
59	90810.5
60	87707.0
61	80609.5
62	73512.0
63	65554.0
64	57596.0
65	47877.5
66	38159.0
67	32748.0
68	27337.0
69	23909.0
70	20481.0
71	17362.5
72	14244.0
73	11500.5
74	8757.0
75	6687.5
76	4618.0
77	3540.5
78	2463.0
79	1876.5
80	1290.0
81	910.0
82	530.0
83	397.0
84	264.0
85	181.5
86	99.0
87	64.5
88	30.0
89	21.0
90	12.0
91	10.5
92	9.0
93	6.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1960424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.904706642369742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95963429546833	20.97481090666003
2	5.231713056957128	2.815154100689739
3	2.208312454192784	1.7824199626424522
4	1.458826866486787	1.5699723553933793
5	1.1103445759429746	1.493674754384608
6	0.9000892319660759	1.4529982042801894
7	0.7720311249760083	1.4539889655380736
8	0.6729608871680549	1.4484652200836317
9	0.6036313625921684	1.4616472257608588
>10	8.363669799011504	50.45332359163156
>50	0.6674255223489469	11.242375719503519
>100	0.04864743811686654	2.1138061360498437
>500	0.0019381319802474466	0.3705414630097206
>1k	5.814395940742339E-4	0.17364242496796647
>5k	0.0	0.0
>10k+	1.9381319802474464E-4	1.193178969404507
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22882	1.1671964840259046	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007498377901923257	0.0
2	0.0	0.0	0.0	0.03682876765434416	0.0
3	0.0	0.0	0.0	0.0680465042256165	0.0
4	0.0	0.0	0.0	0.10094755012181038	0.0
5	0.0	0.0	0.0	0.17598233851452544	0.0
6	0.0	0.0	0.0	0.3355906681411776	0.0
7	0.0	0.0	0.0	0.4496986366214656	0.0
8	0.0	0.0	0.0	0.7460120871811404	0.0
9	0.0	0.0	0.0	0.9266872880560532	0.0
10	0.0	0.0	0.0	1.141130694176362	0.0
11	0.0	0.0	0.0	1.2523821377416313	0.0
12	0.0	0.0	0.0	1.3556761190436355	0.0
13	0.0	0.0	0.0	1.4118884486213186	0.0
14	0.0	0.0	0.0	1.4374441447360367	0.0
15	0.0	0.0	0.0	1.4614695596462806	0.0
16	0.0	0.0	0.0	1.5061537708169253	0.0
17	0.0	0.0	0.0	1.5524192725655266	0.0
18	0.0	0.0	0.0	1.6381660293895606	0.0
19	0.0	0.0	0.0	1.6756069095256945	0.0
20	0.0	0.0	0.0	1.7191179051062424	0.0
21	0.0	0.0	0.0	1.7651283599874312	0.0
22	0.0	0.0	0.0	1.8173109490599992	0.0
23	0.0	0.0	0.0	1.8734722692642	0.0
24	0.0	0.0	0.0	1.9195847428923538	0.0
25	0.0	0.0	0.0	1.9628406916054895	0.0
26	0.0	0.0	0.0	2.0048724153550457	0.0
27	0.0	0.0	0.0	2.0470061578515666	0.0
28	0.0	0.0	0.0	2.095720109527327	0.0
29	0.0	0.0	0.0	2.150198120406606	0.0
30	0.0	0.0	0.0	2.22747732123255	0.0
31	0.0	0.0	0.0	2.2881784756766903	0.0
32	0.0	0.0	0.0	2.3517871644093318	0.0
33	0.0	0.0	0.0	2.4182013686835093	0.0
34	0.0	0.0	0.0	2.4876251259931523	0.0
35	0.0	0.0	0.0	2.5699032454203783	0.0
36	0.0	0.0	0.0	2.6386638808747493	0.0
37	0.0	0.0	0.0	2.7189016253626765	0.0
38	0.0	0.0	0.0	2.809239225800133	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	16270	0.0	43.02643	1
GTTTTTT	18235	0.0	38.4623	2
TAGGGAC	1670	0.0	38.071857	5
CGTTAGG	220	0.0	38.000004	2
TTACGGG	515	0.0	37.59223	3
AGTACGG	210	0.0	36.666664	2
CGTAAGG	385	0.0	36.57143	2
TTAGGGA	1940	0.0	36.40206	4
GCGTAAG	200	0.0	36.3	1
AGGGATC	1840	0.0	36.108696	6
TAGCGGG	1145	0.0	35.93013	3
ATAGGGA	2125	0.0	35.924706	4
TCGTTAG	80	0.0	35.75	1
CGACGGT	160	0.0	35.75	28
TATGGGA	1700	0.0	35.717648	4
TAGGGAT	2435	0.0	35.597534	5
ACGGGAT	900	0.0	35.444447	5
GGCGATT	720	0.0	35.444447	8
GACCGAT	480	0.0	35.291664	9
TAGTAGG	1090	0.0	35.119267	2
>>END_MODULE