FastQCFastQC Report
Thu 26 May 2016
SRR1546540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546540_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1389114
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59400.42761069285890146No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC207.857049E-444.00000439
GATCGTC207.857049E-444.00000410
TCGTCGT207.857049E-444.00000440
TTAGACG351.4463694E-744.01
TAGTTCG254.443321E-544.01
CGTTTTT46050.042.3279041
TAATGCG1200.042.166671
AATGCGG2250.041.0666662
ACGCGCC501.3478711E-939.60000227
TCGATAG452.3515895E-839.111111
TTACGGG2950.038.779663
TGACGGG5500.038.43
AATGCGC357.2913044E-637.71428739
TACGACA357.2913044E-637.71428729
GCGTAAG1350.037.481481
CGTTAGG1950.037.230772
TATACGG950.037.0526352
CGTAAGG2500.036.962
TAGGGAT19400.036.8556755
AGGGATC13550.036.693736
Produced by FastQC (version 0.11.5)