FastQCFastQC Report
Thu 26 May 2016
SRR1546539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546539_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1462081
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55370.37870678847478356No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21610.1478030286967685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTTA207.8571844E-444.00000433
ATACGTG207.8571844E-444.0000041
TCGAATA207.8571844E-444.00000415
TAATCCG207.8571844E-444.0000041
ACGTTAG351.4464422E-744.01
TAGTACG351.4464422E-744.01
TTCGTAG850.041.4117621
CGTTTTT43650.041.278351
ACGGGAT2100.040.857145
CGTTAGG2500.039.62
TTTAGCG900.039.111111
TACGTAG850.038.823531
CGGTCTA1650.038.66666831
GCGCGAC13500.038.622229
GTATTAG1600.038.5000041
AACTTAA31200.037.7948744
GTTGATC7050.037.75886516
CTCACGA1750.037.71428724
TATCGCG700.037.7142871
CTACGGG2100.037.7142833
Produced by FastQC (version 0.11.5)