##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546539_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1462081
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.563103548982582	31.0	30.0	31.0	27.0	33.0
2	30.886707371205837	31.0	31.0	33.0	28.0	34.0
3	31.050200365096053	31.0	31.0	34.0	28.0	34.0
4	34.868356130747884	35.0	35.0	37.0	32.0	37.0
5	34.655379558314486	35.0	35.0	37.0	32.0	37.0
6	34.68572466231351	35.0	35.0	37.0	31.0	37.0
7	35.31282329775163	35.0	35.0	37.0	33.0	37.0
8	34.31089932773902	35.0	35.0	37.0	32.0	37.0
9	36.43113001263268	39.0	37.0	39.0	32.0	39.0
10	35.87451721211068	38.0	35.0	39.0	30.0	39.0
11	35.55116440197226	37.0	35.0	39.0	30.0	39.0
12	34.576602801076	35.0	33.0	39.0	27.0	39.0
13	33.96661744458754	35.0	33.0	39.0	24.0	39.0
14	34.816387737751874	37.0	33.0	40.0	25.0	41.0
15	35.3189481294128	37.0	33.0	40.0	27.0	41.0
16	35.583049092355346	37.0	34.0	40.0	29.0	41.0
17	35.51636126863012	36.0	34.0	40.0	29.0	41.0
18	35.45792811752564	36.0	34.0	40.0	29.0	41.0
19	35.43023129361506	36.0	34.0	40.0	29.0	41.0
20	35.233045911957	36.0	34.0	40.0	29.0	41.0
21	34.98777495911649	36.0	33.0	40.0	27.0	41.0
22	34.91415660281476	36.0	33.0	40.0	27.0	41.0
23	34.921044730079934	35.0	33.0	40.0	27.0	41.0
24	34.84951449338306	35.0	33.0	40.0	27.0	41.0
25	34.616202522295275	35.0	33.0	39.0	27.0	41.0
26	34.447541552075435	35.0	33.0	39.0	26.0	41.0
27	34.23914885700587	35.0	33.0	39.0	25.0	41.0
28	34.24144763525413	35.0	33.0	39.0	25.0	41.0
29	34.0955692605266	35.0	33.0	39.0	25.0	41.0
30	34.169273795364276	35.0	33.0	39.0	25.0	41.0
31	34.05998983640441	35.0	33.0	39.0	24.0	41.0
32	33.83979410169478	35.0	32.0	39.0	24.0	41.0
33	33.69732456683317	35.0	32.0	39.0	23.0	41.0
34	33.5894974355046	35.0	32.0	39.0	23.0	41.0
35	33.38185162107982	35.0	32.0	39.0	23.0	41.0
36	33.27285423994977	35.0	31.0	39.0	22.0	41.0
37	33.250401311555244	35.0	31.0	39.0	22.0	41.0
38	33.302550953059374	35.0	31.0	39.0	23.0	41.0
39	33.2518314648778	35.0	31.0	39.0	23.0	41.0
40	33.1072177259673	35.0	31.0	39.0	22.0	41.0
41	33.1734897040588	35.0	32.0	39.0	22.0	41.0
42	33.022751133487134	35.0	31.0	39.0	22.0	41.0
43	33.008173965737875	35.0	31.0	39.0	22.0	41.0
44	32.6979353401077	35.0	31.0	38.0	21.0	40.0
45	32.61256524091346	35.0	31.0	38.0	21.0	40.0
46	32.583332250401995	35.0	31.0	38.0	21.0	40.0
47	32.5248095009784	35.0	31.0	38.0	21.0	40.0
48	32.56310560085248	35.0	31.0	38.0	22.0	40.0
49	32.57560969604283	35.0	31.0	38.0	21.0	40.0
50	31.946636335469787	34.0	30.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	7.0
13	7.0
14	26.0
15	93.0
16	244.0
17	667.0
18	1527.0
19	2724.0
20	4636.0
21	7629.0
22	10602.0
23	15063.0
24	20283.0
25	26344.0
26	33715.0
27	40353.0
28	46435.0
29	53452.0
30	62797.0
31	74771.0
32	89722.0
33	110856.0
34	161457.0
35	172906.0
36	114616.0
37	131398.0
38	147346.0
39	132401.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.2784018122115	23.573933318331886	28.986355749100085	13.161309120356531
2	31.388548240487363	25.347159288712458	29.81175461551036	13.452537855289822
3	29.328744440287508	25.827502033061094	30.743098364591294	14.100655162060105
4	26.56761150716	27.813506912407725	30.183485046314125	15.435396534118151
5	23.580978071666344	32.51488802603959	29.28633912895387	14.617794773340192
6	20.47882436062024	41.273910268993305	27.151436890295404	11.095828480091049
7	87.83692558757005	4.279174683208386	6.217097411155743	1.6668023180658254
8	87.08997654712701	5.564807968915539	5.552633540822978	1.7925819431344774
9	86.20821965404105	3.834808057829901	7.262388335529973	2.6945839525990696
10	54.141665201859546	20.426775260741366	12.697449730897262	12.734109806501829
11	47.1060768863011	20.856163235826195	17.960974802353633	14.076785075519071
12	40.59966581878843	20.27486849223812	23.396514967365007	15.728950721608449
13	23.71496517634796	35.748361410893104	23.86495686627485	16.671716546484085
14	17.08386881438169	38.63541076041614	27.770280853112787	16.51043957208937
15	16.25046765534878	21.44074097125946	45.1578264131741	17.150964960217664
16	18.902919879267973	17.3456874140352	42.93250510744617	20.818887599250658
17	19.288055860106248	17.081953735805335	28.188930709037326	35.441059695051095
18	24.553564405802415	21.627324341127473	31.877030068785515	21.942081184284593
19	31.330753904879415	23.387213157136983	24.24961407746903	21.032418860514568
20	32.24451996845592	23.58501341580938	22.465923570581932	21.704543045152764
21	25.552893444344054	27.23392206040568	26.567201133179353	20.64598336207091
22	23.748137073117014	23.19714160843346	25.605421313867016	27.44930000458251
23	21.618227717889773	28.34131624718466	24.528805175636645	25.51165085928892
24	22.81911877659309	23.589322342606188	34.46724223897308	19.12431664182764
25	20.98064334328946	23.592673730114814	32.55435232384526	22.872330602750463
26	18.855111310522467	31.318647872450295	26.22570158561667	23.600539231410572
27	20.00894615277813	29.952239308218903	28.27955496309712	21.759259575905848
28	18.488100180496154	26.426990023124574	35.62518082103522	19.45972897534405
29	19.17157804526562	25.10264479190961	34.38851883035208	21.33725833247269
30	23.05939274226257	27.150479351007228	29.9087396662702	19.88138824046
31	31.960335986857093	23.90483153806116	23.706826092398437	20.42800638268331
32	32.259498618749575	24.189562684967523	24.515946790909666	19.034991905373232
33	30.697341665749022	23.96884987904227	24.01228112532753	21.321527329881178
34	22.324207755931443	26.090688545983433	27.29588853148355	24.289215166601576
35	22.38309642215445	24.663818215269877	29.883022896816254	23.07006246575942
36	31.77867710475685	23.56045937263394	24.286958109708014	20.3739054129012
37	22.583153737720412	31.016339040039504	25.314876535568136	21.08563068667194
38	23.67160232572614	32.188777502751215	21.20758015458788	22.932040016934767
39	23.395899406394037	28.549375855373267	24.052087401450397	24.0026373367823
40	25.572454604088286	23.780830200241983	24.95771438107738	25.68900081459235
41	20.446473211812478	22.831977161320065	25.836393469308472	30.885156157558985
42	24.104341688319593	25.194500167911354	22.4713268279938	28.229831315775257
43	23.268067911422143	24.59029287707042	24.79041858829983	27.351220623207606
44	21.406064369894693	27.629180599433273	27.89633406083521	23.06842096983683
45	20.13958186995112	33.30054901199044	23.34508142845711	23.21478768960133
46	24.018847109017898	29.52401405941258	25.272813202551703	21.184325629017817
47	22.376804021117845	26.167702063018396	25.894940157214275	25.560553758649483
48	24.239491519279714	23.911944687059062	29.227176880077092	22.621386913584132
49	23.734799918745953	22.646966891711198	30.145457057440726	23.47277613210212
50	21.437936749058363	29.83603507603204	26.06989626429726	22.65613191061234
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	709.0
1	1331.0
2	1953.0
3	5994.5
4	10036.0
5	7434.5
6	4833.0
7	5021.0
8	5209.0
9	5489.5
10	5770.0
11	5765.5
12	5761.0
13	5668.0
14	5575.0
15	5172.0
16	4769.0
17	4510.0
18	4251.0
19	4344.5
20	4438.0
21	4176.5
22	3915.0
23	4220.5
24	4526.0
25	5113.0
26	5700.0
27	7494.0
28	9288.0
29	10979.5
30	12671.0
31	13865.5
32	15060.0
33	17968.0
34	20876.0
35	24140.0
36	27404.0
37	30786.5
38	34169.0
39	44879.0
40	55589.0
41	78510.0
42	101431.0
43	115034.5
44	128638.0
45	129660.5
46	130683.0
47	124783.5
48	118884.0
49	117219.0
50	115554.0
51	107965.5
52	100377.0
53	91254.5
54	82132.0
55	79842.5
56	77553.0
57	76754.5
58	75956.0
59	73104.5
60	70253.0
61	66106.0
62	61959.0
63	56366.5
64	50774.0
65	41464.0
66	32154.0
67	28238.0
68	24322.0
69	21120.5
70	17919.0
71	15719.5
72	13520.0
73	11030.0
74	8540.0
75	6496.5
76	4453.0
77	3275.5
78	2098.0
79	1780.0
80	1462.0
81	1004.5
82	547.0
83	373.0
84	199.0
85	136.5
86	74.0
87	69.5
88	65.0
89	44.0
90	23.0
91	14.0
92	5.0
93	4.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1462081.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.908980422654782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47624273520233	19.001959316221118
2	6.730762925739027	3.2185135804205
3	2.51451997946705	1.80358826884356
4	1.3122101773292485	1.2549442976069347
5	0.8258881170667522	0.9873071411126099
6	0.5896198020881597	0.8458324982961238
7	0.4650961325326138	0.7783982029162302
8	0.38781900746952364	0.7417885645697799
9	0.31880143483981993	0.6859995537869559
>10	5.413888061999906	32.91785557534011
>50	1.684741729863885	27.32738441844973
>100	0.27614402576724756	9.163696706377426
>500	0.0025595223806844153	0.4177617612194505
>1k	0.0014219568781580086	0.47848136616231657
>5k	2.8439137563160166E-4	0.3764887486770992
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5537	0.37870678847478356	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2161	0.1478030286967685	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016209772235601175	0.0
2	0.0	0.0	0.0	0.06716454149941077	0.0
3	0.0	0.0	0.0	0.10998022681369911	0.0
4	0.0	0.0	0.0	0.16278167899042528	0.0
5	0.0	0.0	0.0	0.28473114690636153	0.0
6	0.0	0.0	0.0	0.46837350324640015	0.0
7	0.0	0.0	0.0	0.5856036703848829	0.0
8	0.0	0.0	0.0	0.9407823506358403	0.0
9	0.0	0.0	0.0	1.1255190375909405	0.0
10	0.0	0.0	0.0	1.387474428571331	0.0
11	0.0	0.0	0.0	1.5431429585638552	0.0
12	0.0	0.0	0.0	1.7055142635736324	0.0
13	0.0	0.0	0.0	1.8317042626229327	0.0
14	0.0	0.0	0.0	1.9041352702073278	0.0
15	0.0	0.0	0.0	1.9445571073011687	0.0
16	0.0	0.0	0.0	2.019518754432894	0.0
17	0.0	0.0	0.0	2.0937280492667645	0.0
18	0.0	0.0	0.0	2.244061717510863	0.0
19	6.839566344135517E-5	0.0	0.0	2.292622638554225	0.0
20	6.839566344135517E-5	0.0	0.0	2.3578037058138364	0.0
21	6.839566344135517E-5	0.0	0.0	2.4211380901605315	0.0
22	6.839566344135517E-5	0.0	0.0	2.4783852604609455	0.0
23	6.839566344135517E-5	0.0	0.0	2.543908306037764	0.0
24	6.839566344135517E-5	0.0	0.0	2.6036861158855085	0.0
25	6.839566344135517E-5	0.0	0.0	2.6504003540159538	0.0
26	6.839566344135517E-5	0.0	0.0	2.702381058231384	0.0
27	6.839566344135517E-5	0.0	0.0	2.757507962965116	0.0
28	6.839566344135517E-5	0.0	0.0	2.8115405370837867	0.0
29	6.839566344135517E-5	0.0	0.0	2.874601338776716	0.0
30	6.839566344135517E-5	0.0	0.0	2.9564709479160185	0.0
31	6.839566344135517E-5	0.0	0.0	3.0263713159530834	0.0
32	6.839566344135517E-5	0.0	0.0	3.093125483471846	0.0
33	6.839566344135517E-5	0.0	0.0	3.1680871306035714	0.0
34	6.839566344135517E-5	0.0	0.0	3.2518718183192314	0.0
35	6.839566344135517E-5	0.0	0.0	3.3514559042898444	0.0
36	6.839566344135517E-5	0.0	0.0	3.426827925402218	0.0
37	6.839566344135517E-5	0.0	0.0	3.5052093557060107	0.0
38	6.839566344135517E-5	0.0	0.0	3.5931661788915936	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTTA	20	7.8571844E-4	44.000004	33
ATACGTG	20	7.8571844E-4	44.000004	1
TCGAATA	20	7.8571844E-4	44.000004	15
TAATCCG	20	7.8571844E-4	44.000004	1
ACGTTAG	35	1.4464422E-7	44.0	1
TAGTACG	35	1.4464422E-7	44.0	1
TTCGTAG	85	0.0	41.411762	1
CGTTTTT	4365	0.0	41.27835	1
ACGGGAT	210	0.0	40.85714	5
CGTTAGG	250	0.0	39.6	2
TTTAGCG	90	0.0	39.11111	1
TACGTAG	85	0.0	38.82353	1
CGGTCTA	165	0.0	38.666668	31
GCGCGAC	1350	0.0	38.62222	9
GTATTAG	160	0.0	38.500004	1
AACTTAA	3120	0.0	37.79487	44
GTTGATC	705	0.0	37.758865	16
CTCACGA	175	0.0	37.714287	24
TATCGCG	70	0.0	37.714287	1
CTACGGG	210	0.0	37.714283	3
>>END_MODULE