FastQCFastQC Report
Thu 26 May 2016
SRR1546538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546538_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1668085
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106030.6356390711504509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG351.4466059E-744.01
CGTTTTT87150.042.5611041
CGATGAA21800.040.66972419
TTTAGCG603.6379788E-1240.3333321
TAATGCG603.6379788E-1240.3333321
CCGATGA21900.039.37899418
GTCGAAT1750.038.97142843
CGAACCG404.1260319E-738.537
CTAACCG404.1260319E-738.51
TGCGTAG1600.037.1251
TCGATAG950.037.052631
CGTAAGG2800.036.9285742
TAGGGCG4850.036.742275
ATAGCGC301.3013474E-436.66666441
CAATTCG301.3013474E-436.6666641
AACGGGA4200.036.6666644
CGCAAGG2400.036.6666642
GATGAAT24950.036.59318520
GCGATAC1450.036.413799
CGACCCG7150.036.30769332
Produced by FastQC (version 0.11.5)