##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546538_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1668085
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.584222027054977	31.0	30.0	31.0	28.0	33.0
2	30.913564956222256	31.0	31.0	33.0	28.0	34.0
3	31.05937527164383	31.0	31.0	34.0	28.0	34.0
4	34.887122059127684	35.0	35.0	37.0	32.0	37.0
5	34.671205604030966	35.0	35.0	37.0	32.0	37.0
6	34.713064981700576	35.0	35.0	37.0	32.0	37.0
7	35.36299469151752	35.0	35.0	37.0	33.0	37.0
8	34.34242919275697	35.0	35.0	37.0	32.0	37.0
9	36.436595257435926	39.0	37.0	39.0	32.0	39.0
10	35.91035948407905	38.0	35.0	39.0	30.0	39.0
11	35.569895418998435	37.0	35.0	39.0	30.0	39.0
12	34.988792537550545	37.0	34.0	39.0	30.0	39.0
13	34.62669528231475	37.0	33.0	39.0	27.0	39.0
14	35.50997880803436	38.0	33.0	40.0	27.0	41.0
15	35.840721545964385	38.0	33.0	40.0	29.0	41.0
16	35.997549885047825	38.0	34.0	40.0	30.0	41.0
17	35.8581810878942	38.0	34.0	40.0	29.0	41.0
18	35.79926682393283	37.0	34.0	40.0	29.0	41.0
19	35.76432615843917	37.0	34.0	40.0	29.0	41.0
20	35.496798424540714	37.0	34.0	40.0	29.0	41.0
21	35.333086143691716	37.0	34.0	40.0	28.0	41.0
22	35.25828120269651	37.0	33.0	40.0	27.0	41.0
23	35.13745222815384	36.0	33.0	40.0	27.0	41.0
24	35.110025568241426	36.0	33.0	40.0	27.0	41.0
25	34.96404379872728	36.0	33.0	40.0	27.0	41.0
26	34.71520695887799	36.0	33.0	40.0	26.0	41.0
27	34.46911578246912	36.0	33.0	40.0	25.0	41.0
28	34.475783308404544	36.0	33.0	40.0	25.0	41.0
29	34.307675568091554	36.0	33.0	39.0	25.0	41.0
30	34.387438889505034	36.0	33.0	39.0	25.0	41.0
31	34.30997341262586	36.0	33.0	40.0	24.0	41.0
32	33.987519221142804	36.0	32.0	40.0	23.0	41.0
33	33.75631217833624	36.0	32.0	40.0	23.0	41.0
34	33.61788158277306	36.0	32.0	40.0	23.0	41.0
35	33.35312648935756	35.0	32.0	40.0	21.0	41.0
36	33.26848092273475	35.0	31.0	40.0	20.0	41.0
37	33.23811556365533	35.0	31.0	40.0	20.0	41.0
38	33.27885389533507	35.0	31.0	40.0	20.0	41.0
39	33.191293609138626	35.0	31.0	40.0	19.0	41.0
40	33.11526331092241	35.0	31.0	40.0	19.0	41.0
41	33.181071707976514	35.0	31.0	40.0	20.0	41.0
42	32.998267474379304	35.0	31.0	39.0	18.0	41.0
43	33.02904588195446	35.0	31.0	39.0	19.0	41.0
44	32.690750171603966	35.0	31.0	39.0	18.0	41.0
45	32.67097899687366	35.0	31.0	39.0	18.0	41.0
46	32.62813525689638	35.0	31.0	39.0	18.0	41.0
47	32.559686107122836	35.0	31.0	39.0	18.0	41.0
48	32.640093880108026	35.0	31.0	39.0	19.0	41.0
49	32.67095981319897	35.0	31.0	39.0	19.0	41.0
50	32.06363284844597	35.0	30.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	2.0
11	2.0
12	4.0
13	11.0
14	36.0
15	99.0
16	280.0
17	776.0
18	1702.0
19	3233.0
20	5769.0
21	9376.0
22	14179.0
23	19179.0
24	25973.0
25	32782.0
26	40563.0
27	46331.0
28	51412.0
29	57732.0
30	66072.0
31	78457.0
32	93114.0
33	114017.0
34	158958.0
35	172888.0
36	137260.0
37	160300.0
38	190245.0
39	187331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.23027903254331	22.660415985995918	26.978241516469488	15.131063464991293
2	33.31808630855142	26.07517003030421	27.570117829726904	13.036625831417464
3	29.482670247619275	26.13062283996319	31.462965016770728	12.923741895646804
4	26.846593548889896	26.436842247247593	31.920495658194874	14.796068545667637
5	24.147150774690736	31.232341277572782	29.93894196039171	14.681565987344769
6	20.10598980267792	42.05013533483006	27.839828306111496	10.004046556380521
7	84.71444800474795	4.017481123563847	9.617855205220357	1.6502156664678358
8	83.15625402782231	7.353222407731021	7.7846752413695945	1.7058483230770614
9	80.82256000143877	5.240080691331677	9.566119232533115	4.371240074696433
10	46.825851200628264	27.52203874502798	14.706984356312777	10.945125698030976
11	38.88548844932962	23.008120089803576	24.344982419960616	13.761409040906189
12	34.0442483446587	21.99468252517108	26.63377465776624	17.327294472403985
13	24.345941603695255	31.44318185224374	27.111568055584694	17.099308488476307
14	17.488257492873565	35.55724078808933	29.790927920339787	17.16357379869731
15	17.607915663770132	23.943923720913503	40.82297964432268	17.625180970993686
16	20.440265334200593	21.000188839297756	39.01335963095406	19.546186195547588
17	20.438586762664972	22.103250134135852	29.990617984095536	27.467545119103644
18	22.49022082208041	23.650653294046766	32.31909644892197	21.54002943495086
19	28.059001789477158	24.50096967480674	27.07925555352395	20.360772982192156
20	30.831762170392995	24.504566613811647	25.91948252037516	18.744188695420196
21	24.948848529901056	27.439788739782443	28.391718647431034	19.219644082885466
22	22.88036880614597	23.99326173426414	29.68116133170672	23.44520812788317
23	23.258107350644604	27.474019609312478	27.634862731815225	21.6330103082277
24	21.625276889367147	25.04482685234865	34.18830575180522	19.141590506478988
25	20.675924788005407	26.040099875006366	32.453202324821575	20.830773012166645
26	19.68221043891648	30.442513421078658	29.043304148169906	20.831971991834948
27	21.693139138593057	28.595125548158517	29.2622378356019	20.44949747764652
28	18.671830272438157	28.60345845685322	34.06061441713102	18.664096853577604
29	19.76086350515711	25.928055225003522	34.57527643974977	19.73580483008959
30	21.242622528228477	27.95343162968314	30.758204767742654	20.04574107434573
31	26.576703225555054	25.10597481543207	26.857863957771936	21.459458001240943
32	29.059490373691986	25.960667471981342	26.769798901135132	18.210043253191536
33	27.98592397869413	26.26143152177497	25.982908544828355	19.769735954702547
34	21.275654418090205	27.208145867866445	28.630915091257343	22.88528462278601
35	22.21685345770749	25.87571976248213	30.062496815210256	21.844929964600126
36	26.840059109697645	27.79714462991994	25.92146083682786	19.441335423554555
37	22.756034614543026	30.837097629916943	26.546488937913836	19.860378817626202
38	22.449275666407885	31.197091275324702	24.5218319210352	21.831801137232215
39	22.847217018317412	29.483329686436843	25.914986346619028	21.754466948626717
40	23.895664789264337	25.424603662283396	26.792879259749952	23.886852288702315
41	20.459029366009528	25.740534804881047	26.87117263209009	26.92926319701934
42	22.239034581571083	26.90102722583082	25.3544034027043	25.5055347898938
43	22.294007799362742	27.0183473863742	25.870743996858675	24.816900817404388
44	21.812017972705227	28.692722493158325	28.136815569949974	21.358443964186478
45	20.618793406810802	31.445100219713023	25.597616428419418	22.338489945056754
46	22.526310110096308	30.527760875494952	26.135478707619814	20.810450306788923
47	22.176987383736442	27.610703291498933	27.25017010524044	22.962139219524186
48	22.62048996304145	25.03415593330076	29.770964908862556	22.57438919479523
49	21.98473099392417	25.013833227923037	30.625177973544517	22.37625780460828
50	20.549192637065858	30.221601417193966	27.014450702452216	22.21475524328796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	647.0
1	1346.5
2	2046.0
3	11054.0
4	20062.0
5	15045.0
6	10028.0
7	9970.0
8	9912.0
9	9998.0
10	10084.0
11	10028.5
12	9973.0
13	9591.5
14	9210.0
15	8675.0
16	8140.0
17	7722.0
18	7304.0
19	7252.0
20	7200.0
21	7316.0
22	7432.0
23	8181.0
24	8930.0
25	10360.0
26	11790.0
27	14019.5
28	16249.0
29	19145.5
30	22042.0
31	25520.0
32	28998.0
33	33408.0
34	37818.0
35	42091.5
36	46365.0
37	51431.0
38	56497.0
39	65667.0
40	74837.0
41	93336.5
42	111836.0
43	124886.0
44	137936.0
45	138141.5
46	138347.0
47	132137.5
48	125928.0
49	123123.0
50	120318.0
51	113334.0
52	106350.0
53	101021.0
54	95692.0
55	90424.0
56	85156.0
57	82757.5
58	80359.0
59	77999.0
60	75639.0
61	67244.0
62	58849.0
63	49738.0
64	40627.0
65	34235.0
66	27843.0
67	22878.0
68	17913.0
69	16288.5
70	14664.0
71	12720.0
72	10776.0
73	8669.0
74	6562.0
75	5134.0
76	3706.0
77	2981.0
78	2256.0
79	1603.5
80	951.0
81	677.0
82	403.0
83	323.0
84	243.0
85	155.0
86	67.0
87	58.5
88	50.0
89	38.5
90	27.0
91	20.5
92	14.0
93	10.0
94	6.0
95	4.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1668085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.414799371544156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16489286059131	21.428748556713554
2	6.908732907954637	3.7880305316632246
3	2.6116952887052998	2.1479730707838876
4	1.3858962317995263	1.519762685782523
5	0.9216708030935961	1.263371007671046
6	0.7130112648794276	1.172823646579226
7	0.6012730178872706	1.1538645407031685
8	0.5106452659317456	1.1199390012438095
9	0.42530341382849246	1.0493646985926846
>10	6.70495702445036	43.86345493828884
>50	0.9729609693236309	17.40487710249859
>100	0.07701928955101961	3.047434081100151
>500	0.0010787011131407945	0.2038719504828604
>1k	6.472206678844768E-4	0.209372412158203
>5k	0.0	0.0
>10k+	2.1574022262815895E-4	0.627111775738256
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10603	0.6356390711504509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010191327180569336	0.0
2	0.0	0.0	0.0	0.048558676566242125	0.0
3	0.0	0.0	0.0	0.07643495385427002	0.0
4	0.0	0.0	0.0	0.12883036535907944	0.0
5	0.0	0.0	0.0	0.2540038427298369	0.0
6	0.0	0.0	0.0	0.38906890236408814	0.0
7	0.0	0.0	0.0	0.4997946747318032	0.0
8	0.0	0.0	0.0	0.7872500502072736	0.0
9	0.0	0.0	0.0	0.9490523564446656	0.0
10	0.0	0.0	0.0	1.2196021185970738	0.0
11	0.0	0.0	0.0	1.3278699826447693	0.0
12	0.0	0.0	0.0	1.4772628493152327	0.0
13	0.0	0.0	0.0	1.534334281526421	0.0
14	0.0	0.0	0.0	1.5608916811793163	0.0
15	0.0	0.0	0.0	1.5937437240907988	0.0
16	0.0	0.0	0.0	1.6468585233965896	0.0
17	0.0	0.0	0.0	1.7033904147570418	0.0
18	0.0	0.0	0.0	1.8027258802758852	0.0
19	0.0	0.0	0.0	1.8629746086080745	0.0
20	0.0	0.0	0.0	1.9124924689089584	0.0
21	0.0	0.0	0.0	1.9687246153523352	0.0
22	0.0	0.0	0.0	2.0257960475635235	0.0
23	0.0	0.0	0.0	2.0911404394859976	0.0
24	0.0	0.0	0.0	2.1423368713225046	0.0
25	0.0	0.0	0.0	2.1886174865189725	0.0
26	0.0	0.0	0.0	2.23232029542859	0.0
27	0.0	0.0	0.0	2.2847157069333996	0.0
28	0.0	0.0	0.0	2.3347131591016046	0.0
29	0.0	0.0	0.0	2.390225917744	0.0
30	0.0	0.0	0.0	2.4706774534870823	0.0
31	0.0	0.0	0.0	2.538359855762746	0.0
32	0.0	0.0	0.0	2.6056226151545037	0.0
33	0.0	0.0	0.0	2.671806292844789	0.0
34	0.0	0.0	0.0	2.7423062973409627	0.0
35	0.0	0.0	0.0	2.826894312939688	0.0
36	0.0	0.0	0.0	2.9008114094905237	0.0
37	0.0	0.0	0.0	2.98060350641604	0.0
38	5.9948983415113736E-5	0.0	0.0	3.068009124235276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	35	1.4466059E-7	44.0	1
CGTTTTT	8715	0.0	42.561104	1
CGATGAA	2180	0.0	40.669724	19
TTTAGCG	60	3.6379788E-12	40.333332	1
TAATGCG	60	3.6379788E-12	40.333332	1
CCGATGA	2190	0.0	39.378994	18
GTCGAAT	175	0.0	38.971428	43
CGAACCG	40	4.1260319E-7	38.5	37
CTAACCG	40	4.1260319E-7	38.5	1
TGCGTAG	160	0.0	37.125	1
TCGATAG	95	0.0	37.05263	1
CGTAAGG	280	0.0	36.928574	2
TAGGGCG	485	0.0	36.74227	5
ATAGCGC	30	1.3013474E-4	36.666664	41
CAATTCG	30	1.3013474E-4	36.666664	1
AACGGGA	420	0.0	36.666664	4
CGCAAGG	240	0.0	36.666664	2
GATGAAT	2495	0.0	36.593185	20
GCGATAC	145	0.0	36.41379	9
CGACCCG	715	0.0	36.307693	32
>>END_MODULE