FastQCFastQC Report
Thu 26 May 2016
SRR1546537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546537_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2023130
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231761.145551694651357No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAA21910.10829753896190557No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCA254.444044E-544.035
CGTTTTT150000.042.914661
TACGTTG557.8216544E-1140.01
CGGTCTA2350.038.38297731
GTTTTTT174400.037.793582
TATTACG357.2929524E-637.7142871
TAGGGTA6600.037.6666685
CGTTAGG2750.037.62
TCGATAG1000.037.41
AAATGCG1000.037.41
GCGTAAG1650.037.3333361
GTTGATC8500.037.01176516
AACGGGA5300.036.9433944
TAGCATA8250.036.830
GGCGATA6350.036.724418
GGGCGAT25900.036.6949777
GTTAACG301.3014453E-436.6666641
GTAGGGA17600.036.6254
GTAGCAT8450.036.44970329
ATCCTGC8700.036.4137920
Produced by FastQC (version 0.11.5)