FastQCFastQC Report
Thu 26 May 2016
SRR1546536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546536_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2008346
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.29213093759740605No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31980.15923551021586918No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATACG207.8578887E-444.0000041
TAGCGTC254.4440334E-544.032
CCGTACC351.446806E-744.018
ACGTATC254.4440334E-544.010
AATCGAC254.4440334E-544.029
GTATGCG1000.041.81
CGTTTTT52050.041.168111
TAATGCG557.8216544E-1140.01
GCGTATG557.8216544E-1140.01
TCGGTAC501.3496901E-939.636
CGTAAGG3350.039.402982
TTTAGCG950.039.368421
AGGGCGC12300.038.2764246
TATAGCG1150.038.2608681
ACCCGCT22350.037.8970934
TCGATAA357.292927E-637.71428738
ATACGTT357.292927E-637.71428728
TCGACTA357.292927E-637.71428717
CGATACG357.292927E-637.71428710
GTACGTC357.292927E-637.71428715
Produced by FastQC (version 0.11.5)