##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546536_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 2008346 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.61909302480748 31.0 30.0 31.0 28.0 33.0 2 30.93752719899858 31.0 31.0 33.0 28.0 34.0 3 31.11953368592862 31.0 31.0 34.0 28.0 34.0 4 34.92708278354427 35.0 35.0 37.0 32.0 37.0 5 34.7159498413122 35.0 35.0 37.0 32.0 37.0 6 34.75708269391828 35.0 35.0 37.0 32.0 37.0 7 35.375646427458214 35.0 35.0 37.0 33.0 37.0 8 34.32677636224037 35.0 35.0 37.0 32.0 37.0 9 36.46796169584324 39.0 37.0 39.0 32.0 39.0 10 35.94661676822619 38.0 35.0 39.0 30.0 39.0 11 35.61425073169663 37.0 35.0 39.0 30.0 39.0 12 34.67925098563693 35.0 33.0 39.0 28.0 39.0 13 34.049869892936776 35.0 33.0 39.0 25.0 39.0 14 34.910645874764604 37.0 33.0 40.0 25.0 41.0 15 35.43718014724554 37.0 33.0 40.0 27.0 41.0 16 35.702707103258106 37.0 34.0 40.0 30.0 41.0 17 35.64038367890792 37.0 34.0 40.0 29.0 41.0 18 35.61702664779874 37.0 34.0 40.0 29.0 41.0 19 35.600870567123394 37.0 34.0 40.0 29.0 41.0 20 35.3752769692075 36.0 34.0 40.0 29.0 41.0 21 35.13223070128355 36.0 33.0 40.0 28.0 41.0 22 35.06764770612235 36.0 33.0 40.0 27.0 41.0 23 35.04729712908035 36.0 33.0 40.0 28.0 41.0 24 34.97978137233326 36.0 33.0 40.0 27.0 41.0 25 34.7915448832024 35.0 33.0 40.0 27.0 41.0 26 34.57145780657317 35.0 33.0 39.0 26.0 41.0 27 34.3670682243 35.0 33.0 39.0 25.0 41.0 28 34.41552750372695 36.0 33.0 39.0 25.0 41.0 29 34.28497529808111 36.0 33.0 39.0 25.0 41.0 30 34.36640797950154 36.0 33.0 39.0 25.0 41.0 31 34.259527491776815 35.0 33.0 39.0 25.0 41.0 32 33.99885179147418 35.0 32.0 39.0 24.0 41.0 33 33.86617146647042 35.0 32.0 39.0 24.0 41.0 34 33.79639215553495 35.0 32.0 39.0 23.0 41.0 35 33.58055833008854 35.0 32.0 39.0 23.0 41.0 36 33.43956569236576 35.0 32.0 39.0 23.0 41.0 37 33.42564926561459 35.0 32.0 39.0 23.0 41.0 38 33.47122557567272 35.0 32.0 39.0 23.0 41.0 39 33.422394348384195 35.0 32.0 39.0 23.0 41.0 40 33.323006095563215 35.0 32.0 39.0 23.0 41.0 41 33.37957702507436 35.0 32.0 39.0 23.0 41.0 42 33.22057752996744 35.0 32.0 39.0 23.0 41.0 43 33.22493285519527 35.0 32.0 39.0 23.0 41.0 44 32.93756703277224 35.0 31.0 39.0 22.0 40.0 45 32.85321752327537 35.0 31.0 38.0 22.0 40.0 46 32.82830149784947 35.0 31.0 38.0 22.0 40.0 47 32.74665321612909 35.0 31.0 38.0 22.0 40.0 48 32.797425344039326 35.0 31.0 38.0 22.0 40.0 49 32.82382816506718 35.0 31.0 38.0 22.0 40.0 50 32.19910164881948 35.0 30.0 38.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 2.0 10 0.0 11 3.0 12 7.0 13 16.0 14 43.0 15 116.0 16 289.0 17 783.0 18 1664.0 19 3245.0 20 5538.0 21 9131.0 22 13685.0 23 19359.0 24 26323.0 25 34752.0 26 43692.0 27 52345.0 28 60912.0 29 70553.0 30 83812.0 31 100857.0 32 121615.0 33 150763.0 34 220760.0 35 242817.0 36 159325.0 37 184455.0 38 206332.0 39 195150.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.64400755646686 23.88149253166536 27.846944699767867 12.627555212099907 2 31.24685686629694 25.29489440564524 29.7545841204653 13.703664607592517 3 30.097801872784867 25.930541848864685 30.136490425454575 13.835165852895864 4 27.60719517453666 27.304060156964987 29.608493755558058 15.4802509129403 5 23.6969625751738 31.640464342299584 28.606325802426475 16.05624728010014 6 20.185665219040942 42.466736309380956 26.773026161826696 10.574572309751408 7 87.8836116884242 4.358561721934368 6.047613309658794 1.7102132799826326 8 86.53688159311194 6.268840130136939 5.362571987097841 1.831706289653277 9 85.5992941455307 4.109650428760781 6.876006425187692 3.4150490005208263 10 54.632767461383644 20.550642170223657 12.44342359334497 12.373166775047725 11 47.28458144164402 21.041444053962813 18.033147674753256 13.640826829639913 12 40.220260851466826 21.363300945155864 22.755092996923835 15.66134520645347 13 23.4339600845671 37.88804319574416 23.404184338754376 15.27381238093436 14 17.136738390695626 40.7602076534621 27.101505417891143 15.001548537951129 15 15.395604143907473 22.117752618323735 46.04126978120304 16.44537345656575 16 17.470893959506977 17.50360744612731 43.6932679926666 21.33223060169911 17 18.417294629511048 17.968417792551683 27.989997739433342 35.62428983850393 18 22.89685143894528 22.250349292402802 32.784490321886764 22.06830894676515 19 31.479386520051822 23.821194156783744 23.423503718980694 21.27591560418374 20 32.53104793695907 23.87103616607895 22.623392582752174 20.974523314209804 21 24.302784480363442 27.853118934685554 26.911000395350204 20.933096189600796 22 23.65971799679936 22.85876039288051 25.252869774431296 28.228651835888837 23 21.276064980834974 30.01743723442076 23.725393931125414 24.98110385361885 24 21.211683644152952 24.824955460861823 35.155695283581615 18.807665611403614 25 20.2823119123896 24.3434149294992 33.47177229421624 21.902500863894968 26 18.299834789423734 33.277881400913984 25.832799726740312 22.589484082921967 27 20.476800312296785 30.53597338307244 27.96261202004037 21.024614284590402 28 18.491236071872077 26.67379027318998 36.41822673981475 18.41674691512319 29 17.960650206687493 25.457266825537033 35.94126709242332 20.640815875352157 30 21.852459685731443 28.07773162592502 30.151776636097566 19.918032052245977 31 31.807666607247953 24.241888598876887 22.547359867273865 21.40308492660129 32 31.89789010459353 25.058530751175347 24.05322588836784 18.990353255863283 33 29.886782456807744 24.645205557209763 23.583735073538126 21.88427691244437 34 21.52343271527914 26.829341159342068 26.699931187156 24.947294938222797 35 22.132540906795942 23.868198009705498 29.84894037182836 24.1503207116702 36 31.57354360254657 23.69775925064705 24.697387800707645 20.03130934609873 37 22.211959493035565 30.64013870119989 26.08300561755793 21.064896188206614 38 22.287444494126014 32.941534974551196 21.609971588560935 23.161048942761855 39 21.966234901754977 29.592510453875974 24.41411987775015 24.0271347666189 40 25.03229025277517 24.41914889167504 24.67747091387639 25.871089941673397 41 20.114661517487527 23.04463473923318 26.078972447974603 30.761731295304696 42 23.514225138497054 25.894890621436744 22.62324320610094 27.96764103396526 43 22.785914379295203 25.76413625938957 24.12074413472579 27.329205226589444 44 21.10054741563456 28.59193585169089 28.277199247540015 22.030317485134535 45 19.32510633127957 34.38371675000224 23.649908930034965 22.641267988683225 46 23.85639725425798 30.252257330161235 25.52284317542893 20.368502240151845 47 22.61846315326144 26.64381535850894 25.996914874229837 24.74080661399978 48 23.955185012940998 24.2313824410734 29.4403454384852 22.3730871075004 49 22.777200741306526 23.275122912087856 30.59164108176579 23.35603526483982 50 20.875536386658474 31.212998158683813 25.532801618844562 22.37866383581315 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 744.0 1 1574.5 2 2405.0 3 6752.0 4 11099.0 5 8615.0 6 6131.0 7 6343.0 8 6555.0 9 6968.5 10 7382.0 11 7438.0 12 7494.0 13 7415.0 14 7336.0 15 6972.0 16 6608.0 17 6285.0 18 5962.0 19 5810.0 20 5658.0 21 6441.5 22 7225.0 23 6886.0 24 6547.0 25 8964.0 26 11381.0 27 11163.5 28 10946.0 29 14203.0 30 17460.0 31 20964.5 32 24469.0 33 28857.0 34 33245.0 35 37300.5 36 41356.0 37 45450.5 38 49545.0 39 68304.0 40 87063.0 41 119535.5 42 152008.0 43 173132.5 44 194257.0 45 189747.0 46 185237.0 47 177019.0 48 168801.0 49 161093.0 50 153385.0 51 146380.5 52 139376.0 53 126947.0 54 114518.0 55 106499.0 56 98480.0 57 94126.0 58 89772.0 59 87941.5 60 86111.0 61 79508.0 62 72905.0 63 65963.5 64 59022.0 65 50382.0 66 41742.0 67 36400.5 68 31059.0 69 27779.5 70 24500.0 71 21240.5 72 17981.0 73 14323.5 74 10666.0 75 8450.0 76 6234.0 77 4503.0 78 2772.0 79 2127.5 80 1483.0 81 1117.0 82 751.0 83 590.0 84 429.0 85 293.5 86 158.0 87 113.5 88 69.0 89 40.5 90 12.0 91 8.5 92 5.0 93 3.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2008346.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.21468182424294 #Duplication Level Percentage of deduplicated Percentage of total 1 79.28373567819246 16.81979226250231 2 7.30129024514528 3.0978909891441173 3 2.7692171406336055 1.7624418162234523 4 1.5216559773733909 1.291257896237356 5 0.9312332836641032 0.987790890853946 6 0.6218941098158932 0.7915971400868984 7 0.447603408903903 0.6647034732339969 8 0.34968542415004705 0.593477200953495 9 0.2745802883314549 0.5242620106944633 >10 3.873147346395204 20.637788149756613 >50 1.8408691869138825 28.02904356435275 >100 0.7781320224565825 23.087587703806793 >500 0.005100984551028133 0.8220954853411286 >1k 0.0016230405389634965 0.6016796491768496 >5k 2.3186293413764237E-4 0.2885917676357567 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5867 0.29213093759740605 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 3198 0.15923551021586918 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.009908651198548458 0.0 2 0.0 0.0 0.0 0.04152670904316288 0.0 3 0.0 0.0 0.0 0.06487925885280724 0.0 4 0.0 0.0 0.0 0.11183331955748661 0.0 5 0.0 0.0 0.0 0.20917710394523653 0.0 6 0.0 0.0 0.0 0.3439646355757424 0.0 7 0.0 0.0 0.0 0.444544914073571 0.0 8 0.0 0.0 0.0 0.7785013140166087 0.0 9 0.0 0.0 0.0 0.9452554490112759 0.0 10 0.0 0.0 0.0 1.1937684044482375 0.0 11 0.0 0.0 0.0 1.3144149464285537 0.0 12 0.0 0.0 0.0 1.452737725471607 0.0 13 0.0 0.0 0.0 1.5072104109550843 0.0 14 0.0 0.0 0.0 1.5266293756155562 0.0 15 0.0 0.0 0.0 1.554612601613467 0.0 16 0.0 0.0 0.0 1.6159068208366487 0.0 17 0.0 0.0 0.0 1.6794416898283464 0.0 18 0.0 0.0 0.0 1.7987438419475528 0.0 19 0.0 0.0 0.0 1.8454489415668416 0.0 20 0.0 0.0 0.0 1.8936975999155523 0.0 21 0.0 0.0 0.0 1.9495146752601393 0.0 22 0.0 0.0 0.0 2.0039873607436167 0.0 23 0.0 0.0 0.0 2.0667753464791425 0.0 24 0.0 0.0 0.0 2.1149742126107753 0.0 25 0.0 0.0 0.0 2.1543100641025004 0.0 26 0.0 0.0 0.0 2.1987247217361947 0.0 27 0.0 0.0 0.0 2.2411974829038424 0.0 28 0.0 0.0 0.0 2.2900436478574906 0.0 29 0.0 0.0 0.0 2.3429229823944677 0.0 30 0.0 0.0 0.0 2.413378969560026 0.0 31 0.0 0.0 0.0 2.478059059544521 0.0 32 0.0 0.0 0.0 2.5366147068284053 0.0 33 0.0 0.0 0.0 2.6023902255886187 0.0 34 0.0 0.0 0.0 2.66502883467291 0.0 35 0.0 0.0 0.0 2.7479826683250796 0.0 36 0.0 0.0 0.0 2.819085954312653 0.0 37 4.979221707813295E-5 0.0 0.0 2.8879485905317113 0.0 38 4.979221707813295E-5 0.0 0.0 2.962487539497676 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAATACG 20 7.8578887E-4 44.000004 1 TAGCGTC 25 4.4440334E-5 44.0 32 CCGTACC 35 1.446806E-7 44.0 18 ACGTATC 25 4.4440334E-5 44.0 10 AATCGAC 25 4.4440334E-5 44.0 29 GTATGCG 100 0.0 41.8 1 CGTTTTT 5205 0.0 41.16811 1 TAATGCG 55 7.8216544E-11 40.0 1 GCGTATG 55 7.8216544E-11 40.0 1 TCGGTAC 50 1.3496901E-9 39.6 36 CGTAAGG 335 0.0 39.40298 2 TTTAGCG 95 0.0 39.36842 1 AGGGCGC 1230 0.0 38.276424 6 TATAGCG 115 0.0 38.260868 1 ACCCGCT 2235 0.0 37.89709 34 TCGATAA 35 7.292927E-6 37.714287 38 ATACGTT 35 7.292927E-6 37.714287 28 TCGACTA 35 7.292927E-6 37.714287 17 CGATACG 35 7.292927E-6 37.714287 10 GTACGTC 35 7.292927E-6 37.714287 15 >>END_MODULE