FastQCFastQC Report
Thu 26 May 2016
SRR1546535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546535_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1577577
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200311.2697320004031498No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT16330.1035131724156729No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT147500.042.836611
TACGACG452.3519533E-839.111111
GACCGAT9300.038.5591439
TCGACGG800.038.52
ACGGGTA1150.038.260875
AGGGCGA18150.038.0606086
GGACCGA9900.037.777788
GGGCGAT34750.037.7323767
CGTTCGA700.037.71428714
AGTACGG1050.037.7142832
TACGGGA6250.037.6644
GTTTTTT170250.037.629372
TAGGGCG8100.036.6666685
TAGGGAC15150.036.594065
GGCGATT9400.036.510648
TGGGCGA11300.036.2123876
AAGGGCG6750.036.177785
CTATGCG553.426976E-936.0000041
CGAGGGA10750.035.813954
ATAGGGC8800.035.7500044
Produced by FastQC (version 0.11.5)