FastQCFastQC Report
Thu 26 May 2016
SRR1546534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546534_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2169752
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT258391.1908734270091699No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT153250.042.3491061
AGGGCGA22600.037.6725656
CGTAAGG2850.037.0526282
GTTTTTT182100.036.7391552
ACTAACG301.3014761E-436.6666641
GGGCGAT44200.036.533947
TAGGGCG9050.036.464095
TGTTACG850.036.235291
CTACGAA1800.035.44444711
TACGGGA6750.035.24
ACTACGG1250.035.22
AGTACGG2000.035.22
GACCGAT11900.034.9411779
AGGGATT29500.034.8271186
CGTTAGG2850.034.736842
TTAGGGA22650.034.578374
TAGACGG2100.034.5714262
CGGGTAT1850.034.486496
TAAGGGA22050.034.421774
GAGGGAT26400.034.3333325
Produced by FastQC (version 0.11.5)