##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546534_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2169752
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.401781401745453	31.0	31.0	33.0	30.0	34.0
2	31.807036011488872	31.0	31.0	34.0	30.0	34.0
3	31.908778975661736	33.0	31.0	34.0	30.0	34.0
4	35.60472579354691	37.0	35.0	37.0	33.0	37.0
5	35.500808387317996	37.0	35.0	37.0	33.0	37.0
6	35.53104225736398	37.0	35.0	37.0	33.0	37.0
7	36.005881778194	37.0	35.0	37.0	35.0	37.0
8	35.9918446900844	37.0	35.0	37.0	35.0	37.0
9	37.672327528676085	39.0	37.0	39.0	35.0	39.0
10	37.06885902167621	39.0	37.0	39.0	33.0	39.0
11	36.84743095063399	39.0	37.0	39.0	32.0	39.0
12	36.57987779248504	39.0	35.0	39.0	32.0	39.0
13	36.50820416342513	39.0	35.0	39.0	32.0	39.0
14	37.61865964405148	40.0	36.0	41.0	32.0	41.0
15	37.73106557800154	40.0	36.0	41.0	33.0	41.0
16	37.751977645371454	40.0	36.0	41.0	33.0	41.0
17	37.67458769481489	40.0	36.0	41.0	32.0	41.0
18	37.54712934934499	39.0	36.0	41.0	32.0	41.0
19	37.47003574602074	39.0	36.0	41.0	32.0	41.0
20	37.2717352029172	39.0	35.0	41.0	32.0	41.0
21	37.17079186930119	39.0	35.0	41.0	32.0	41.0
22	37.099905426979674	39.0	35.0	41.0	32.0	41.0
23	36.993857823382584	39.0	35.0	41.0	32.0	41.0
24	36.928560038197915	39.0	35.0	41.0	31.0	41.0
25	36.757529431935076	38.0	35.0	41.0	31.0	41.0
26	36.67591918339055	38.0	35.0	41.0	31.0	41.0
27	36.56672698077937	38.0	35.0	41.0	31.0	41.0
28	36.60956367363644	38.0	35.0	41.0	31.0	41.0
29	36.5205134042969	38.0	35.0	41.0	31.0	41.0
30	36.46038487347863	38.0	35.0	40.0	31.0	41.0
31	36.30723050376264	38.0	35.0	40.0	30.0	41.0
32	36.00807235112584	38.0	35.0	41.0	29.0	41.0
33	35.83881199326006	38.0	35.0	41.0	28.0	41.0
34	35.646460056264495	38.0	35.0	41.0	27.0	41.0
35	35.41199915935093	38.0	35.0	41.0	25.0	41.0
36	35.26007027531257	38.0	35.0	41.0	25.0	41.0
37	35.234515281009074	38.0	35.0	40.0	25.0	41.0
38	35.16735737540512	38.0	34.0	40.0	24.0	41.0
39	35.04641590375306	38.0	34.0	40.0	24.0	41.0
40	34.94271050332019	38.0	34.0	40.0	23.0	41.0
41	34.91550508998263	38.0	34.0	40.0	23.0	41.0
42	34.7287501060029	38.0	34.0	40.0	23.0	41.0
43	34.75005115792035	38.0	34.0	40.0	23.0	41.0
44	34.505084452047974	38.0	34.0	40.0	23.0	41.0
45	34.40846603667147	37.0	34.0	40.0	23.0	41.0
46	34.39642687274859	37.0	34.0	40.0	23.0	41.0
47	34.308336620959444	37.0	33.0	40.0	23.0	41.0
48	34.24781449677198	37.0	33.0	40.0	23.0	41.0
49	34.22697939672368	37.0	33.0	40.0	23.0	41.0
50	33.69520387583466	36.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	3.0
12	10.0
13	12.0
14	25.0
15	69.0
16	167.0
17	428.0
18	994.0
19	2010.0
20	3435.0
21	5616.0
22	8188.0
23	11766.0
24	17524.0
25	25949.0
26	37266.0
27	45437.0
28	48232.0
29	49062.0
30	53981.0
31	62369.0
32	75354.0
33	93931.0
34	143767.0
35	206442.0
36	166690.0
37	209126.0
38	314761.0
39	587135.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.087468060865945	22.969998414565353	26.17478863943898	17.76774488512973
2	35.90894258883043	24.631847326330384	26.029057698760045	13.430152386079147
3	28.65857480486249	24.6095867177447	33.09419694047983	13.637641536912975
4	24.7914508201859	27.641776571700362	32.55344389589225	15.013328712221488
5	22.72208989783164	30.987458474516906	31.57614326429933	14.714308363352124
6	20.119303957318625	39.525438851997826	29.642788668935438	10.712468521748107
7	82.87048473742621	3.983081937474882	10.953740335300994	2.192692989797912
8	82.42094027335844	3.5025661918965856	10.718644342763598	3.357849191981388
9	75.84258477466548	5.932198702893234	13.965835726848045	4.259380795593229
10	36.47398412353117	30.171604865440845	19.607217783414878	13.747193227613112
11	28.234517124537735	25.29763770237336	31.163838079190615	15.304007093898289
12	26.731396030514087	22.352231960150284	32.408865160626654	18.507506848708978
13	22.10911661793606	26.86989112119726	33.49940454024239	17.521587720624293
14	18.65176296645884	29.652835900139742	32.965518639918294	18.729882493483128
15	17.80576766376987	23.805762133183887	40.54315884949064	17.845311353555612
16	20.398690725944714	23.319439272322366	38.03807992802864	18.243790073704275
17	20.221066739424597	23.12365652848805	33.10982084588469	23.545455886202664
18	22.046298378800895	23.10512906544158	35.142080753929484	19.706491801828044
19	24.820071602653208	26.09878917037523	30.236911868268816	18.844227358702746
20	25.82977225046918	24.988892739815427	29.560336849557	19.620998160158397
21	23.32054538951917	26.849796658788655	30.91625217997264	18.913405771719532
22	22.243417680914686	24.266598210302377	30.77264129725425	22.71734281152869
23	20.471464019851116	27.74870123405809	30.017808486868546	21.762026259222253
24	20.209590773507756	24.762691773068994	34.871128128929016	20.156589324494227
25	20.46372119947349	26.075560709242346	32.483343718544795	20.977374372739373
26	19.442913291472944	27.659612711498827	31.320630191837594	21.57684380519064
27	20.3492841578208	26.048046043971844	31.44774149303699	22.15492830517036
28	19.596064435013773	26.11164778278808	34.45329235783628	19.838995424361862
29	20.50639888798351	24.468879392667915	33.1036450248692	21.921076694479368
30	21.047290197220697	25.38704884244835	32.32595245908288	21.23970850124807
31	24.31005939849347	24.725775111625662	29.298463603213637	21.665701886667232
32	24.498007145517093	24.73038393327901	29.55176444128177	21.219844479922127
33	23.651320519580118	25.402626659636677	28.798544718474737	22.147508102308468
34	20.912205634561	25.989928802923103	31.81133143326979	21.286534129246107
35	21.995094370232174	25.804930701757623	30.24336421858351	21.9566107094267
36	22.94534121871993	27.07867074209403	29.535034418680105	20.44095362050594
37	22.60839026764349	28.205711989204296	28.81108071337185	20.374817029780363
38	21.580715215379453	29.05198382119247	29.040646119925228	20.326654843502855
39	21.59186856378056	29.04954114571619	28.039656145034087	21.31893414546916
40	22.604011887072808	26.904226842514724	29.507473665193075	20.9842876052194
41	20.0397556955818	26.97252957941737	29.42059737702742	23.567117347973408
42	22.559951552066778	27.18753110954616	27.697912019438164	22.554605318948894
43	21.624452932869747	26.577599651941792	29.304040277414195	22.493907137774272
44	21.680173586658753	27.430738628193453	29.744643627474478	21.14444415767332
45	20.561105601008776	28.02822626733378	29.091850128493945	22.318818003163496
46	22.156495304532502	27.588775122686833	28.99174652218318	21.262983050597487
47	21.984724521512135	26.244151405321897	29.303671571681928	22.46745250148404
48	22.08519683355517	25.232468964194986	30.123258326297197	22.559075875952644
49	22.16898521121308	24.577785848336582	30.03389327443874	23.219335666011602
50	20.42832544917576	26.212995770945252	30.925377646846275	22.433301133032714
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2304.0
1	2682.0
2	3060.0
3	22837.0
4	42614.0
5	29551.5
6	16489.0
7	16669.0
8	16849.0
9	16950.5
10	17052.0
11	16921.0
12	16790.0
13	16372.0
14	15954.0
15	14857.0
16	13760.0
17	13113.0
18	12466.0
19	12238.0
20	12010.0
21	12203.5
22	12397.0
23	13527.5
24	14658.0
25	16136.0
26	17614.0
27	21587.5
28	25561.0
29	28828.5
30	32096.0
31	35772.0
32	39448.0
33	45267.0
34	51086.0
35	58750.0
36	66414.0
37	72382.5
38	78351.0
39	87405.0
40	96459.0
41	109395.0
42	122331.0
43	137736.5
44	153142.0
45	162305.5
46	171469.0
47	180544.0
48	189619.0
49	188850.5
50	188082.0
51	171357.0
52	154632.0
53	137828.0
54	121024.0
55	109643.5
56	98263.0
57	91670.0
58	85077.0
59	79121.5
60	73166.0
61	68359.5
62	63553.0
63	55067.5
64	46582.0
65	38270.0
66	29958.0
67	26088.0
68	22218.0
69	19363.0
70	16508.0
71	14169.0
72	11830.0
73	9953.5
74	8077.0
75	6468.0
76	4859.0
77	3913.5
78	2968.0
79	2373.5
80	1779.0
81	1221.5
82	664.0
83	486.0
84	308.0
85	227.5
86	147.0
87	88.0
88	29.0
89	21.5
90	14.0
91	13.5
92	13.0
93	9.0
94	5.0
95	4.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2169752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.215232304542976
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.54144385867176	21.103084078434286
2	6.39576958890027	3.481247102565043
3	2.5306835201351903	2.0661941966927326
4	1.4757337800842563	1.6064975057861748
5	1.0855910944990526	1.477230691226739
6	0.8214150490162503	1.3413000826454884
7	0.6729210705123766	1.281959227963125
8	0.578182445215565	1.2588295648760237
9	0.4912929395686828	1.2033586331949144
>10	7.69323269757636	50.19313855762888
>50	0.6713015275049539	11.226972371117029
>100	0.03902431215830791	1.7386434813364702
>500	0.002385681309834984	0.4986847460140241
>1k	8.520290392267799E-4	0.32454160258589615
>5k	0.0	0.0
>10k+	1.7040580784535598E-4	1.1983181579332622
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25839	1.1908734270091699	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05129618500178822	0.0
2	0.0	0.0	0.0	0.17246210626836617	0.0
3	0.0	0.0	0.0	0.33118992400974856	0.0
4	0.0	0.0	0.0	0.5324110773950202	0.0
5	0.0	0.0	0.0	0.8835571991637754	0.0
6	0.0	0.0	0.0	1.7292759725535454	0.0
7	0.0	0.0	0.0	2.249980642949056	0.0
8	0.0	0.0	0.0	3.7222226318952583	0.0
9	0.0	0.0	0.0	4.6139835336019965	0.0
10	0.0	0.0	0.0	5.758999185160332	0.0
11	0.0	0.0	0.0	6.283805706827324	0.0
12	0.0	0.0	0.0	6.800777231683621	0.0
13	0.0	0.0	0.0	7.111503987552495	0.0
14	0.0	0.0	0.0	7.264470778227189	0.0
15	0.0	0.0	0.0	7.381949642171087	0.0
16	0.0	0.0	0.0	7.567823419450702	0.0
17	0.0	0.0	0.0	7.748811845777766	0.0
18	0.0	0.0	0.0	8.084564503224332	0.0
19	0.0	0.0	0.0	8.219142095502159	0.0
20	4.608821653350245E-5	0.0	0.0	8.392894671833464	0.0
21	4.608821653350245E-5	0.0	0.0	8.55194510709058	0.0
22	9.21764330670049E-5	0.0	0.0	8.721872361449604	0.0
23	9.21764330670049E-5	0.0	0.0	8.90788440337882	0.0
24	9.21764330670049E-5	0.0	0.0	9.06002160615591	0.0
25	9.21764330670049E-5	0.0	0.0	9.190727788244924	0.0
26	9.21764330670049E-5	0.0	0.0	9.319175647723796	0.0
27	9.21764330670049E-5	0.0	0.0	9.474216408142498	0.0
28	9.21764330670049E-5	0.0	0.0	9.611697558061936	0.0
29	9.21764330670049E-5	0.0	0.0	9.755216264347261	0.0
30	1.3826464960050734E-4	0.0	0.0	9.987546963892648	0.0
31	1.3826464960050734E-4	0.0	0.0	10.14798004564577	0.0
32	1.3826464960050734E-4	0.0	0.0	10.32311526847308	0.0
33	1.3826464960050734E-4	0.0	0.0	10.478524734624049	0.0
34	1.3826464960050734E-4	0.0	0.0	10.642552697266785	0.0
35	1.3826464960050734E-4	0.0	0.0	10.81644353824769	0.0
36	1.3826464960050734E-4	0.0	0.0	10.974157415225335	0.0
37	1.3826464960050734E-4	0.0	0.0	11.144545551749694	0.0
38	1.3826464960050734E-4	0.0	0.0	11.331940240174914	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	15325	0.0	42.349106	1
AGGGCGA	2260	0.0	37.672565	6
CGTAAGG	285	0.0	37.052628	2
GTTTTTT	18210	0.0	36.739155	2
ACTAACG	30	1.3014761E-4	36.666664	1
GGGCGAT	4420	0.0	36.53394	7
TAGGGCG	905	0.0	36.46409	5
TGTTACG	85	0.0	36.23529	1
CTACGAA	180	0.0	35.444447	11
TACGGGA	675	0.0	35.2	4
ACTACGG	125	0.0	35.2	2
AGTACGG	200	0.0	35.2	2
GACCGAT	1190	0.0	34.941177	9
AGGGATT	2950	0.0	34.827118	6
CGTTAGG	285	0.0	34.73684	2
TTAGGGA	2265	0.0	34.57837	4
TAGACGG	210	0.0	34.571426	2
CGGGTAT	185	0.0	34.48649	6
TAAGGGA	2205	0.0	34.42177	4
GAGGGAT	2640	0.0	34.333332	5
>>END_MODULE