FastQCFastQC Report
Thu 26 May 2016
SRR1546533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546533_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2115417
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159130.7522393929896564No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA23810.11255464052713957No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT111850.042.5838171
TATAGCG1200.042.1666641
TTAGCGG4650.039.2688142
CGAATAT1800.037.8888914
CGACGGT700.037.71428328
CGTTCGA651.0913936E-1137.23076614
TAGGGCG9800.036.8163265
GGGCGAT45950.036.2916227
TACACGC5900.036.1694935
TACGGGA7850.036.1528664
AGGGCGA24500.036.097966
TAGGGAT24750.036.088895
TATCGTG553.426976E-936.01
GTTTTTT138050.035.904382
GACCGAT12100.035.6363649
ACACGCG5950.035.495836
ACGTAGG3600.035.4444472
TAGCGCG751.8189894E-1235.21
AAGCGAC505.9497324E-835.215
TAACGCG250.002353190735.21
Produced by FastQC (version 0.11.5)