##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546533_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2115417
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.45028852467386	31.0	31.0	33.0	30.0	34.0
2	31.843049857309456	31.0	31.0	34.0	30.0	34.0
3	31.960557658371847	33.0	31.0	34.0	30.0	34.0
4	35.63834317300088	37.0	35.0	37.0	33.0	37.0
5	35.53097001678629	37.0	35.0	37.0	33.0	37.0
6	35.56077785136453	37.0	35.0	37.0	33.0	37.0
7	36.02400425069856	37.0	35.0	37.0	35.0	37.0
8	36.013496157022466	37.0	35.0	37.0	35.0	37.0
9	37.701495733465315	39.0	37.0	39.0	35.0	39.0
10	37.09759068779347	39.0	37.0	39.0	33.0	39.0
11	36.88268554143226	39.0	37.0	39.0	32.0	39.0
12	36.52962607372447	39.0	35.0	39.0	32.0	39.0
13	36.42071752283356	39.0	35.0	39.0	32.0	39.0
14	37.51206026991369	40.0	36.0	41.0	32.0	41.0
15	37.658191269144574	40.0	36.0	41.0	33.0	41.0
16	37.69790495207328	40.0	36.0	41.0	33.0	41.0
17	37.63511165883606	40.0	35.0	41.0	33.0	41.0
18	37.53111655999739	39.0	36.0	41.0	32.0	41.0
19	37.48795958432782	39.0	36.0	41.0	33.0	41.0
20	37.31378115993206	39.0	35.0	41.0	32.0	41.0
21	37.21097306110332	39.0	35.0	41.0	32.0	41.0
22	37.14980923382955	39.0	35.0	41.0	32.0	41.0
23	37.03425991187553	39.0	35.0	41.0	32.0	41.0
24	36.989523578566306	39.0	35.0	41.0	32.0	41.0
25	36.84386766297141	38.0	35.0	41.0	31.0	41.0
26	36.745722474575935	38.0	35.0	41.0	31.0	41.0
27	36.63277405825896	38.0	35.0	41.0	31.0	41.0
28	36.68965693288841	38.0	35.0	41.0	31.0	41.0
29	36.610513671772516	38.0	35.0	41.0	31.0	41.0
30	36.58634964170185	38.0	35.0	41.0	31.0	41.0
31	36.44495624266988	38.0	35.0	41.0	31.0	41.0
32	36.19302624494367	38.0	35.0	41.0	30.0	41.0
33	36.06779467121613	38.0	35.0	41.0	30.0	41.0
34	35.925684628609865	38.0	35.0	41.0	29.0	41.0
35	35.72642935175429	38.0	35.0	41.0	28.0	41.0
36	35.575456281196566	38.0	35.0	41.0	27.0	41.0
37	35.53074216572902	38.0	35.0	41.0	27.0	41.0
38	35.474816549172104	38.0	35.0	41.0	27.0	41.0
39	35.37065741648101	38.0	35.0	40.0	26.0	41.0
40	35.2638846147119	38.0	35.0	40.0	25.0	41.0
41	35.249078077750156	38.0	35.0	40.0	25.0	41.0
42	35.05227054524001	38.0	34.0	40.0	24.0	41.0
43	35.08229724919484	38.0	34.0	40.0	25.0	41.0
44	34.75009040770685	38.0	34.0	40.0	23.0	41.0
45	34.69841501699192	38.0	34.0	40.0	23.0	41.0
46	34.72086260061255	38.0	34.0	40.0	23.0	41.0
47	34.64952914720833	37.0	34.0	40.0	23.0	41.0
48	34.61941120828659	37.0	34.0	40.0	23.0	41.0
49	34.59928231644163	37.0	34.0	40.0	24.0	41.0
50	34.065123803013776	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	1.0
11	3.0
12	3.0
13	10.0
14	19.0
15	56.0
16	161.0
17	365.0
18	853.0
19	1798.0
20	3043.0
21	4810.0
22	7527.0
23	11323.0
24	16278.0
25	23582.0
26	32602.0
27	38574.0
28	41441.0
29	43109.0
30	48779.0
31	58661.0
32	71783.0
33	92506.0
34	145475.0
35	223113.0
36	154425.0
37	197221.0
38	301943.0
39	595950.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.57578340346135	24.03086483657832	24.970159547739286	16.423192212221043
2	33.206786179746125	26.087433352383947	27.099810581081652	13.60596988678828
3	28.77692672413997	25.845589782061886	32.494066181750455	12.883417312047696
4	25.29118372406008	28.578904301137793	31.367810696425337	14.762101278376793
5	23.03555280117348	31.75047756541618	30.916363062223667	14.297606571186675
6	20.302758274136966	40.18498480441445	28.74246543352918	10.769791487919402
7	84.26461543988727	4.3801293078385966	9.163157902200842	2.192097350073295
8	84.10667967592205	3.941634202618207	8.847522734288322	3.1041633871714183
9	77.72325740031398	6.360353537860385	11.708991655073207	4.207397406752428
10	37.684437630972994	29.953337805264873	17.942561679328474	14.41966288443366
11	30.753889185914645	26.18268643960032	28.137289243681035	14.926135130803997
12	28.500527319199946	23.568213737527874	29.992951744266023	17.938307199006154
13	22.504924560973087	30.035543819492798	30.888425308107102	16.571106311427013
14	18.53658167633143	32.74191329652735	31.10738922869581	17.61411579844541
15	17.550251321607043	24.6898838385056	40.966012847585134	16.793851992302226
16	19.400288453765853	23.784908601944675	38.36699809068377	18.447804853605696
17	19.98939216239635	23.278341811567177	31.29609906699247	25.43616695904401
18	21.65445394454143	24.171688135247095	34.0217555214882	20.15210239872328
19	25.599822635442564	26.357923756876307	29.15179371253989	18.89045989514124
20	26.944994769352803	25.3903603875737	28.271730821866324	19.392914021207165
21	23.415619709967352	27.75462237469019	29.89557141688849	18.93418649845397
22	22.487197559630086	25.72651160504052	29.022977502780776	22.76331333254862
23	19.802951380271598	29.494941186536742	28.796402789615478	21.905704643576186
24	19.395750341422048	26.220740402483294	34.29980944655357	20.0836998095411
25	19.66671346595021	26.794669797964183	32.39758402244097	21.141032713644638
26	18.77459621436341	29.697974441918547	30.356284363792103	21.171144979925945
27	19.68018598697089	29.13799028749414	30.364462420411677	20.817361305123292
28	18.87117291768006	28.196710152182764	34.078954645821604	18.853162284315577
29	19.50031601334394	25.937675645038304	33.54374102127382	21.018267320343934
30	20.444621556884528	27.81683233140322	31.559593214954784	20.178952896757473
31	23.56249382509453	27.026349887516265	27.914354474791498	21.496801812597706
32	24.541733379281723	27.923714331500598	28.57403528476891	18.960517004448768
33	23.464215329648955	28.401917919729303	27.305491068664	20.82837568195774
34	20.393756881031024	28.264356389307636	29.8851715761006	21.45671515356074
35	20.06488555211573	27.798679882027987	29.99474808040212	22.14168648545417
36	24.482312470779995	27.00748835808732	28.569875348453756	19.94032382267893
37	22.04662248625212	28.648205058388015	28.63411800132078	20.671054454039087
38	21.62202535008464	29.34121263089027	28.063828550115648	20.972933468909442
39	21.677049962253307	27.853751766200237	28.289883271241557	22.179315000304907
40	23.406827117301223	25.68760674609309	29.298856915681398	21.60670922092429
41	20.326252459916887	25.594197267016384	29.172498850108514	24.907051422958215
42	22.471692342455412	27.50237896358023	27.414925756954776	22.611002937009584
43	22.04307708598352	27.174689434754473	28.61312923173067	22.169104247531337
44	21.669628257691038	28.716607647570196	28.995512468700024	20.618251626038745
45	20.07911442519371	29.7461918855715	28.119231338312968	22.05546235092183
46	21.842312886773623	29.040326328095123	28.4247030254555	20.69265775967575
47	21.39686879702678	27.359381152746714	29.196229395906336	22.047520654320166
48	22.25967740639316	25.59334637095192	29.68970184129181	22.457274381363106
49	21.529703127090308	25.21313764614731	30.102197344542468	23.15496188221991
50	19.95200000756352	28.406314216062366	29.485534057824058	22.156151718550056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2308.0
1	2585.5
2	2863.0
3	15184.5
4	27506.0
5	19841.0
6	12176.0
7	12599.0
8	13022.0
9	13529.5
10	14037.0
11	14160.0
12	14283.0
13	13841.5
14	13400.0
15	12975.5
16	12551.0
17	12315.0
18	12079.0
19	11962.5
20	11846.0
21	11525.0
22	11204.0
23	12604.0
24	14004.0
25	16056.0
26	18108.0
27	21213.5
28	24319.0
29	28527.0
30	32735.0
31	38549.5
32	44364.0
33	50719.0
34	57074.0
35	62544.0
36	68014.0
37	71659.0
38	75304.0
39	88633.5
40	101963.0
41	121261.0
42	140559.0
43	154202.0
44	167845.0
45	177760.0
46	187675.0
47	188049.5
48	188424.0
49	185889.0
50	183354.0
51	168400.0
52	153446.0
53	136114.0
54	118782.0
55	105521.0
56	92260.0
57	83690.5
58	75121.0
59	71052.5
60	66984.0
61	58761.0
62	50538.0
63	44072.0
64	37606.0
65	30291.5
66	22977.0
67	19602.5
68	16228.0
69	13710.0
70	11192.0
71	9225.0
72	7258.0
73	6035.5
74	4813.0
75	4005.0
76	3197.0
77	2586.5
78	1976.0
79	1529.5
80	1083.0
81	822.0
82	561.0
83	369.0
84	177.0
85	141.5
86	106.0
87	82.0
88	58.0
89	34.5
90	11.0
91	13.0
92	15.0
93	13.0
94	11.0
95	5.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2115417.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.568806698126462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21876472441232	19.46310517378446
2	6.570698928750621	3.2286846370412126
3	2.319767363677249	1.7098174782842628
4	1.379770014564036	1.3559721110277974
5	0.8903286390220785	1.093715611496973
6	0.6968806412500833	1.0272915459923826
7	0.566257490850928	0.9738589583857794
8	0.447090566865101	0.8787585371091561
9	0.3963791042332315	0.8764705431974505
>10	5.612041726040724	34.477858150176885
>50	1.770244564865649	29.14097259443179
>100	0.1282931753740618	4.226495511512564
>500	0.0021285367240790726	0.37015781692982863
>1k	0.0011610200313158577	0.4203140584994368
>5k	0.0	0.0
>10k+	1.9350333855264296E-4	0.7565272721300236
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15913	0.7522393929896564	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2381	0.11255464052713957	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05833365241935751	0.0
2	0.0	0.0	0.0	0.19584791083743772	0.0
3	0.0	0.0	0.0	0.35137280262000353	0.0
4	0.0	0.0	0.0	0.5338427364439257	0.0
5	0.0	0.0	0.0	0.8241873824404361	0.0
6	0.0	0.0	0.0	1.4906753609335655	0.0
7	0.0	0.0	0.0	1.881945734576209	0.0
8	0.0	0.0	0.0	3.030560877595292	0.0
9	0.0	0.0	0.0	3.743517235608866	0.0
10	0.0	0.0	0.0	4.710276980850584	0.0
11	0.0	0.0	0.0	5.2064912024437735	0.0
12	0.0	0.0	0.0	5.702752696040545	0.0
13	4.727200358132699E-5	0.0	0.0	5.974141268600943	0.0
14	4.727200358132699E-5	0.0	0.0	6.095488501794209	0.0
15	4.727200358132699E-5	0.0	0.0	6.202985037938147	0.0
16	4.727200358132699E-5	0.0	0.0	6.389520364070063	0.0
17	4.727200358132699E-5	0.0	0.0	6.578324746373883	0.0
18	4.727200358132699E-5	0.0	0.0	6.905494283160247	0.0
19	4.727200358132699E-5	0.0	0.0	7.051422958215803	0.0
20	4.727200358132699E-5	0.0	0.0	7.233798348032563	0.0
21	4.727200358132699E-5	0.0	0.0	7.399770352606602	0.0
22	4.727200358132699E-5	0.0	0.0	7.569004125427752	0.0
23	4.727200358132699E-5	0.0	0.0	7.757855779735154	0.0
24	4.727200358132699E-5	0.0	0.0	7.907093495041403	0.0
25	4.727200358132699E-5	0.0	0.0	8.035011536732474	0.0
26	4.727200358132699E-5	0.0	0.0	8.151631569567607	0.0
27	4.727200358132699E-5	0.0	0.0	8.302854709024272	0.0
28	4.727200358132699E-5	0.0	0.0	8.427369166457488	0.0
29	4.727200358132699E-5	0.0	0.0	8.571265145359048	0.0
30	4.727200358132699E-5	0.0	0.0	8.793018114159052	0.0
31	4.727200358132699E-5	0.0	0.0	8.946179405762551	0.0
32	4.727200358132699E-5	0.0	0.0	9.110591434218406	0.0
33	9.454400716265398E-5	0.0	0.0	9.252596532976714	0.0
34	9.454400716265398E-5	0.0	0.0	9.395168895777996	0.0
35	9.454400716265398E-5	0.0	0.0	9.549133811442378	0.0
36	9.454400716265398E-5	0.0	0.0	9.697567902687744	0.0
37	9.454400716265398E-5	0.0	0.0	9.85063465028408	0.0
38	9.454400716265398E-5	0.0	0.0	9.998170573461403	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11185	0.0	42.583817	1
TATAGCG	120	0.0	42.166664	1
TTAGCGG	465	0.0	39.268814	2
CGAATAT	180	0.0	37.88889	14
CGACGGT	70	0.0	37.714283	28
CGTTCGA	65	1.0913936E-11	37.230766	14
TAGGGCG	980	0.0	36.816326	5
GGGCGAT	4595	0.0	36.291622	7
TACACGC	590	0.0	36.16949	35
TACGGGA	785	0.0	36.152866	4
AGGGCGA	2450	0.0	36.09796	6
TAGGGAT	2475	0.0	36.08889	5
TATCGTG	55	3.426976E-9	36.0	1
GTTTTTT	13805	0.0	35.90438	2
GACCGAT	1210	0.0	35.636364	9
ACACGCG	595	0.0	35.4958	36
ACGTAGG	360	0.0	35.444447	2
TAGCGCG	75	1.8189894E-12	35.2	1
AAGCGAC	50	5.9497324E-8	35.2	15
TAACGCG	25	0.0023531907	35.2	1
>>END_MODULE