FastQCFastQC Report
Thu 26 May 2016
SRR1546532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546532_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1481031
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141800.9574411339127946No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCC17700.11951134041083543No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15160.102361125459224No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATG207.8572176E-444.0000041
CGTTTTT76500.041.9869271
GCGATAT1950.040.6153879
TAGTACG404.1256135E-738.5000041
TTTAAGC12600.037.88888544
TAGCATA12650.037.73913230
AGGGCGA18150.037.6969686
GCCAGTA12800.037.6406325
CAGTAGC13000.037.5692327
ATGGGCG7150.037.538465
GCGCGAC12850.037.4941649
TGATCCT13400.037.26865418
TACGGGA3450.036.9855084
CATATGC12950.036.86486433
ACAACGA2450.036.81632613
TCGTTTA1500.036.66666838
CACGCTA301.3012768E-436.66666438
CTCAAAG12500.036.60799844
GGTACCT14150.036.53718
GGGCGAT35100.036.4786347
Produced by FastQC (version 0.11.5)