FastQCFastQC Report
Thu 26 May 2016
SRR1546531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546531_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1483284
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119440.8052402641705836No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA15430.1040259316489627No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGCG1250.044.01
TTGCGTA254.4434673E-544.012
GCGTCAA207.857222E-444.039
CGTTTTT83150.042.042091
TACGCGG800.041.252
GCGTAAG850.038.8235321
TTCACGC404.1256135E-738.542
CGTTTCG357.2916373E-637.71428742
TACGGGA5700.037.43864
GCGCGAC4000.037.49
TATTGCG1000.037.41
GTACGAG1000.037.41
CGTAAGG1650.037.3333322
CGAGGGT2600.037.2307664
TGGGCGA8950.037.117326
ACGGGAT6000.037.0333335
TAGACGG1800.036.6666682
CGCAAAC301.3012778E-436.66666411
TAATGCG301.3012778E-436.6666641
GTTTTTT101000.036.2237632
Produced by FastQC (version 0.11.5)