FastQCFastQC Report
Thu 26 May 2016
SRR1546530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546530_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1625917
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103160.6344727313878875No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA25010.1538208899962298No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT64350.041.948721
GCGTAAG1650.041.3333321
TAATACG557.8216544E-1140.01
TATCGCG557.8216544E-1140.01
CGTAAGG2850.039.3684232
TACGGGA4150.039.2289124
TAACGGG3200.039.1875043
CGGGTAT900.039.1111076
ATTGCGG3150.038.41272
TAAACGG1150.038.2608682
TGCGTAG1500.038.133331
CATACGA1450.037.93103418
TAGGGCG5700.037.8245625
ACGGGAT4900.037.2653055
ATTGGGC4400.037.04
ACGGGAC4350.036.919545
GTACGGG5900.036.9152533
TCGTTGA601.9826984E-1036.66666424
CGCATAA301.301333E-436.66666435
ATTCGCG301.301333E-436.6666641
Produced by FastQC (version 0.11.5)