##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546530_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1625917
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.450364932527307	31.0	31.0	33.0	30.0	34.0
2	31.83702858140975	31.0	31.0	34.0	30.0	34.0
3	31.967017996613603	33.0	31.0	34.0	30.0	34.0
4	35.641771381934014	37.0	35.0	37.0	33.0	37.0
5	35.53376156347464	37.0	35.0	37.0	33.0	37.0
6	35.56167258230278	37.0	35.0	37.0	33.0	37.0
7	36.02410701161252	37.0	35.0	37.0	35.0	37.0
8	36.01356280794161	37.0	35.0	37.0	35.0	37.0
9	37.7106076140418	39.0	37.0	39.0	35.0	39.0
10	37.08903160493432	39.0	37.0	39.0	33.0	39.0
11	36.882224000364104	39.0	37.0	39.0	32.0	39.0
12	36.48800400020419	39.0	35.0	39.0	32.0	39.0
13	36.34611422354278	39.0	35.0	39.0	32.0	39.0
14	37.42171525360766	40.0	35.0	41.0	32.0	41.0
15	37.5617014890674	40.0	35.0	41.0	32.0	41.0
16	37.62324337589188	40.0	35.0	41.0	33.0	41.0
17	37.56185094319083	39.0	35.0	41.0	33.0	41.0
18	37.49285480132135	39.0	36.0	41.0	32.0	41.0
19	37.474050028383985	39.0	36.0	41.0	32.0	41.0
20	37.323860935090785	39.0	35.0	41.0	32.0	41.0
21	37.22152975828409	39.0	35.0	41.0	32.0	41.0
22	37.14418694189187	39.0	35.0	41.0	32.0	41.0
23	37.043392743910054	39.0	35.0	41.0	32.0	41.0
24	37.00821813167585	39.0	35.0	41.0	32.0	41.0
25	36.846511230278054	38.0	35.0	41.0	31.0	41.0
26	36.73843252761365	38.0	35.0	41.0	31.0	41.0
27	36.647163416090734	38.0	35.0	41.0	31.0	41.0
28	36.71310036121155	38.0	35.0	41.0	31.0	41.0
29	36.63721887402617	38.0	35.0	41.0	31.0	41.0
30	36.60874140561911	38.0	35.0	40.0	31.0	41.0
31	36.49083440298613	38.0	35.0	40.0	31.0	41.0
32	36.30250375634181	38.0	35.0	41.0	30.0	41.0
33	36.235893345109254	38.0	35.0	41.0	30.0	41.0
34	36.13918853176392	38.0	35.0	41.0	30.0	41.0
35	35.95839517023317	38.0	35.0	41.0	29.0	41.0
36	35.83185673069413	38.0	35.0	41.0	29.0	41.0
37	35.78549704566715	38.0	35.0	41.0	29.0	41.0
38	35.74542919472519	38.0	35.0	40.0	29.0	41.0
39	35.64416941332183	38.0	35.0	40.0	28.0	41.0
40	35.54414278219614	38.0	35.0	40.0	27.0	41.0
41	35.5375059120484	38.0	35.0	40.0	28.0	41.0
42	35.34815307300434	38.0	34.0	40.0	27.0	41.0
43	35.37239662295185	38.0	35.0	40.0	27.0	41.0
44	35.11753798010599	38.0	34.0	40.0	26.0	41.0
45	35.038188296204545	38.0	34.0	40.0	26.0	41.0
46	35.027865506049814	37.0	34.0	40.0	26.0	41.0
47	34.938321574840536	37.0	34.0	40.0	26.0	41.0
48	34.89567610154762	37.0	34.0	40.0	26.0	41.0
49	34.899346645615985	37.0	34.0	40.0	26.0	41.0
50	34.3765512015681	36.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	3.0
13	4.0
14	10.0
15	40.0
16	107.0
17	248.0
18	607.0
19	1147.0
20	2078.0
21	3272.0
22	5021.0
23	7449.0
24	10783.0
25	15349.0
26	20793.0
27	25457.0
28	28592.0
29	31688.0
30	37497.0
31	45587.0
32	56734.0
33	72921.0
34	117883.0
35	180310.0
36	123037.0
37	156485.0
38	234398.0
39	448412.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.3023149398155	24.49079504058325	27.673552832032634	14.533337187568614
2	31.69602138362536	26.32348391707572	28.16662843183262	13.813866267466299
3	29.41743028703187	25.493429246388345	30.842226263702265	14.246914202877514
4	25.484142179459347	29.005047613131545	30.005283172511266	15.505527034897845
5	23.377023550402633	32.37274719435248	29.258197066639934	14.992032188604954
6	20.49249746450772	41.17774769560808	27.352257218541904	10.977497621342295
7	85.60154054604263	4.427101752426477	7.616501949361498	2.3548557521693914
8	85.08896825606719	3.8941717197126295	7.413908582049392	3.6029514421707876
9	78.91638995102456	6.07773951560873	10.526121567091062	4.479748966275646
10	39.60466616684615	29.236055715021124	16.24123494618729	14.918043171945431
11	32.34623907616441	25.281179789620257	26.492065708151152	15.880515426064184
12	29.699855527680686	22.85479517097121	28.750053046988256	18.695296254359846
13	22.711860445520895	29.62402139838626	30.24773097273723	17.41638718335561
14	18.912712026505655	33.43774620721722	29.797954016102913	17.851587750174208
15	17.398612598306062	25.22631844060921	40.03193274933468	17.343136211750046
16	18.86972090211247	23.022884932010673	38.15287004195171	19.954524123925143
17	19.569633628284837	23.372164753797396	30.71128477037881	26.34691684753896
18	21.78665946662714	24.288570695798125	33.15981074064666	20.76495909692807
19	26.208656407430393	26.44323172708078	27.401706237157246	19.946405628331583
20	26.197278212848502	26.100471303270705	26.803459217167912	20.898791266712877
21	23.305187165150496	28.452313371469764	28.56910900125898	19.67339046212076
22	22.38386092279003	25.64355991111477	27.626440956088167	24.34613821000703
23	19.98976577525175	29.57426486099844	26.884705676858044	23.551263686891765
24	20.37963807500629	25.411198726626267	33.27180907758514	20.937354120782302
25	20.41703235774028	26.488313979126854	30.94801272143658	22.146640941696287
26	18.785706773469986	29.28236804215713	29.411033896564216	22.520891287808663
27	19.857778718101844	28.5836853910747	29.083833922641805	22.47470196818165
28	19.561269117673287	27.22383737915281	33.32365674262585	19.891236760548047
29	20.099180954501367	25.81946064897532	32.39162884698296	21.689729549540353
30	21.184168687577536	26.553015928857377	30.99020429702131	21.272611086543776
31	24.778878626645763	25.82524200189801	27.03895709313575	22.35692227832048
32	26.029372963072532	25.549090144207852	28.21066512005225	20.210871772667364
33	24.04858304575203	25.45498939982791	28.744456205329055	21.751971349091008
34	21.423602803833163	25.640054197108462	30.217040599243383	22.719302399814996
35	20.938645699626733	24.76055050780575	31.46304516159189	22.837758630975628
36	24.819594112122576	24.771190657333676	28.42691232086263	21.98230290968112
37	21.24388883319382	27.367879172184068	29.6290032025005	21.759228792121615
38	21.24690251716416	27.67527493715854	26.660339980454108	24.417482565223196
39	20.706530530156215	26.44452330592521	28.587560127607993	24.261386036310586
40	22.257470707299326	24.36563490018248	28.90768716976328	24.469207222754914
41	19.11536689757226	24.30560723579371	29.73528169027078	26.843744176363245
42	22.203593418360224	26.13891114983114	27.68105629008123	23.976439141727408
43	22.177761841471614	24.924888539820913	29.26939074995833	23.627958868749143
44	22.181390562986916	27.361359774207415	28.874106119808086	21.583143542997583
45	20.064062310683756	27.98912859635517	28.619972606227744	23.32683648673333
46	22.26097642130564	27.65836140467195	28.114903774300902	21.965758399721512
47	21.674415114670676	25.874198990477375	29.270190298766792	23.181195596085164
48	21.92559644803517	24.28955475587007	29.71941372161064	24.06543507448412
49	21.961822159433723	23.06187831236158	30.515456816061338	24.460842712143364
50	20.42644243217827	26.4186302252821	29.877539874421633	23.27738746811799
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1869.0
1	2048.0
2	2227.0
3	9571.5
4	16916.0
5	11922.5
6	6929.0
7	7296.5
8	7664.0
9	8023.0
10	8382.0
11	8352.5
12	8323.0
13	8258.5
14	8194.0
15	7993.5
16	7793.0
17	7745.0
18	7697.0
19	7333.5
20	6970.0
21	7424.5
22	7879.0
23	8686.5
24	9494.0
25	10756.0
26	12018.0
27	14196.5
28	16375.0
29	18028.5
30	19682.0
31	23247.5
32	26813.0
33	31576.0
34	36339.0
35	42305.5
36	48272.0
37	52098.5
38	55925.0
39	64586.0
40	73247.0
41	87055.0
42	100863.0
43	111111.0
44	121359.0
45	135181.5
46	149004.0
47	152939.5
48	156875.0
49	154619.5
50	152364.0
51	135950.0
52	119536.0
53	106040.0
54	92544.0
55	83012.5
56	73481.0
57	68950.5
58	64420.0
59	59801.0
60	55182.0
61	50870.0
62	46558.0
63	40850.0
64	35142.0
65	29314.5
66	23487.0
67	19324.0
68	15161.0
69	13295.5
70	11430.0
71	9388.5
72	7347.0
73	6581.0
74	5815.0
75	4459.5
76	3104.0
77	2229.5
78	1355.0
79	1219.5
80	1084.0
81	740.0
82	396.0
83	319.5
84	243.0
85	169.5
86	96.0
87	72.0
88	48.0
89	30.0
90	12.0
91	7.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1625917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.583810115585454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.64850690248201	20.121284664674118
2	6.763289508762655	3.4606142909782998
3	2.4062009918823484	1.8467936782875414
4	1.3866629562800972	1.4190448707114554
5	0.9111228613838557	1.1654997138806724
6	0.7086689333340812	1.0878270855140173
7	0.5834077854038838	1.0448055801209035
8	0.4899987675478591	1.002882834065226
9	0.43612345688623033	1.0041929737137032
>10	6.103949553761135	39.09294307248849
>50	1.4878286859240866	24.56232315027819
>100	0.07062403168703177	2.7310910177594163
>500	0.002169338798807393	0.3408313713102998
>1k	0.0012051882215596628	0.48371291415921686
>5k	0.0	0.0
>10k+	2.4103764431193255E-4	0.6361527820584499
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10316	0.6344727313878875	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2501	0.1538208899962298	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09397773687094729	0.0
2	0.0	0.0	0.0	0.3182819295203876	0.0
3	0.0	0.0	0.0	0.4967043213152947	0.0
4	0.0	0.0	0.0	0.74807016594328	0.0
5	0.0	0.0	0.0	1.2148221588186852	0.0
6	0.0	0.0	0.0	2.0958019382293194	0.0
7	0.0	0.0	0.0	2.603945957880999	0.0
8	0.0	0.0	0.0	4.132744783405303	0.0
9	0.0	0.0	0.0	5.044107417537304	0.0
10	0.0	0.0	0.0	6.302904760821124	0.0
11	0.0	0.0	0.0	7.013703651539408	0.0
12	6.150375449669326E-5	0.0	0.0	7.675115027396847	0.0
13	6.150375449669326E-5	0.0	0.0	8.02734702939941	0.0
14	6.150375449669326E-5	0.0	0.0	8.180245363078189	0.0
15	6.150375449669326E-5	0.0	0.0	8.32717783257079	0.0
16	6.150375449669326E-5	0.0	0.0	8.611509689609003	0.0
17	6.150375449669326E-5	0.0	0.0	8.902545455887354	0.0
18	6.150375449669326E-5	0.0	0.0	9.39143880038157	0.0
19	6.150375449669326E-5	0.0	0.0	9.581054875494875	0.0
20	6.150375449669326E-5	0.0	0.0	9.822088089367416	0.0
21	6.150375449669326E-5	0.0	0.0	10.044055139345982	0.0
22	6.150375449669326E-5	0.0	0.0	10.279799030331807	0.0
23	6.150375449669326E-5	0.0	0.0	10.53325600261268	0.0
24	6.150375449669326E-5	0.0	0.0	10.731236588337534	0.0
25	6.150375449669326E-5	0.0	0.0	10.899449356885992	0.0
26	6.150375449669326E-5	0.0	0.0	11.064340922691626	0.0
27	6.150375449669326E-5	0.0	0.0	11.25229639643352	0.0
28	6.150375449669326E-5	0.0	0.0	11.42733608173111	0.0
29	6.150375449669326E-5	0.0	0.0	11.608772157496354	0.0
30	6.150375449669326E-5	0.0	0.0	11.850727927686346	0.0
31	6.150375449669326E-5	0.0	0.0	12.040097987781664	0.0
32	6.150375449669326E-5	0.0	0.0	12.230021581667453	0.0
33	6.150375449669326E-5	0.0	0.0	12.412995251295115	0.0
34	6.150375449669326E-5	0.0	0.0	12.603164860198891	0.0
35	6.150375449669326E-5	0.0	0.0	12.804897174948044	0.0
36	6.150375449669326E-5	0.0	0.0	12.983996108042415	0.0
37	6.150375449669326E-5	0.0	0.0	13.178778498533443	0.0
38	6.150375449669326E-5	0.0	0.0	13.381986903390517	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	6435	0.0	41.94872	1
GCGTAAG	165	0.0	41.333332	1
TAATACG	55	7.8216544E-11	40.0	1
TATCGCG	55	7.8216544E-11	40.0	1
CGTAAGG	285	0.0	39.368423	2
TACGGGA	415	0.0	39.228912	4
TAACGGG	320	0.0	39.187504	3
CGGGTAT	90	0.0	39.111107	6
ATTGCGG	315	0.0	38.4127	2
TAAACGG	115	0.0	38.260868	2
TGCGTAG	150	0.0	38.13333	1
CATACGA	145	0.0	37.931034	18
TAGGGCG	570	0.0	37.824562	5
ACGGGAT	490	0.0	37.265305	5
ATTGGGC	440	0.0	37.0	4
ACGGGAC	435	0.0	36.91954	5
GTACGGG	590	0.0	36.915253	3
TCGTTGA	60	1.9826984E-10	36.666664	24
CGCATAA	30	1.301333E-4	36.666664	35
ATTCGCG	30	1.301333E-4	36.666664	1
>>END_MODULE