FastQCFastQC Report
Thu 26 May 2016
SRR1546529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546529_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2013695
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90950.4516572767971317No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA33030.16402682630686374No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTACG254.4440374E-544.01
CCGAATT254.4440374E-544.030
TCCGTCG351.446806E-743.99999616
CGTTTTT64200.041.669781
CACGACG1600.039.87526
CGGTCTA1750.038.97142831
TCGATAG800.038.51
TCGAAAT404.1265957E-738.510
GACCGAT11550.038.476199
TAGCACG1500.038.133331
AGGGCGA26150.037.6902476
AACGCCG1000.037.39999813
AGGGCGC3650.037.3698656
GGGCGAT47900.037.1106457
ACGGGTC1550.036.9032255
GCGCGAC5850.036.8547029
CGTAATC301.3014427E-436.66666834
TTACGAG601.9826984E-1036.6666681
ACGGGAT5300.036.52835
CGAATAT2300.036.34782814
Produced by FastQC (version 0.11.5)