##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546529_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2013695
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.405086172434256	31.0	31.0	33.0	30.0	34.0
2	31.79677955201756	31.0	31.0	34.0	30.0	34.0
3	31.93455712011998	33.0	31.0	34.0	30.0	34.0
4	35.60558674476522	37.0	35.0	37.0	33.0	37.0
5	35.486206699624326	37.0	35.0	37.0	33.0	37.0
6	35.52023121674335	37.0	35.0	37.0	33.0	37.0
7	36.00693054310608	37.0	35.0	37.0	35.0	37.0
8	36.00194120758109	37.0	35.0	37.0	35.0	37.0
9	37.70032204479824	39.0	37.0	39.0	35.0	39.0
10	37.03321903267376	39.0	37.0	39.0	33.0	39.0
11	36.807765326923885	39.0	37.0	39.0	32.0	39.0
12	36.30327283923335	38.0	35.0	39.0	32.0	39.0
13	36.13558855735352	38.0	35.0	39.0	32.0	39.0
14	37.1613342636298	39.0	35.0	41.0	32.0	41.0
15	37.328519959576795	39.0	35.0	41.0	32.0	41.0
16	37.3769011692436	39.0	35.0	41.0	32.0	41.0
17	37.27980503502268	39.0	35.0	41.0	32.0	41.0
18	37.2447346792836	39.0	35.0	41.0	32.0	41.0
19	37.253419708545735	39.0	35.0	41.0	32.0	41.0
20	37.11754957925604	39.0	35.0	41.0	32.0	41.0
21	37.01207332788729	39.0	35.0	41.0	32.0	41.0
22	36.93040952080628	39.0	35.0	41.0	31.0	41.0
23	36.845492986773074	38.0	35.0	41.0	31.0	41.0
24	36.782833547284966	38.0	35.0	41.0	31.0	41.0
25	36.63287389599716	38.0	35.0	41.0	31.0	41.0
26	36.53532535960014	38.0	35.0	41.0	31.0	41.0
27	36.44470190371432	38.0	35.0	40.0	31.0	41.0
28	36.51683248952796	38.0	35.0	40.0	31.0	41.0
29	36.4758739531061	38.0	35.0	40.0	31.0	41.0
30	36.44300303670615	38.0	35.0	40.0	31.0	41.0
31	36.34472002959733	38.0	35.0	40.0	30.0	41.0
32	36.15400892389364	38.0	35.0	40.0	30.0	41.0
33	36.121073449554174	38.0	35.0	41.0	30.0	41.0
34	36.01900933358826	38.0	35.0	41.0	30.0	41.0
35	35.85551337218397	38.0	35.0	41.0	29.0	41.0
36	35.73374865607751	38.0	35.0	40.0	29.0	41.0
37	35.71486843836827	38.0	35.0	40.0	29.0	41.0
38	35.643233955489784	38.0	35.0	40.0	29.0	41.0
39	35.559016137001876	38.0	35.0	40.0	28.0	41.0
40	35.43644295685295	38.0	34.0	40.0	27.0	41.0
41	35.448591767869516	38.0	34.0	40.0	28.0	41.0
42	35.26890616503492	38.0	34.0	40.0	27.0	41.0
43	35.30519815562933	38.0	34.0	40.0	27.0	41.0
44	35.06255068419001	37.0	34.0	40.0	27.0	41.0
45	34.94636377405715	37.0	34.0	40.0	26.0	41.0
46	34.941507030607916	37.0	34.0	40.0	26.0	41.0
47	34.85192345414772	37.0	34.0	40.0	26.0	41.0
48	34.82418588713782	37.0	34.0	40.0	26.0	41.0
49	34.82584403298414	37.0	34.0	40.0	26.0	41.0
50	34.28967594397364	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	3.0
12	6.0
13	5.0
14	25.0
15	63.0
16	141.0
17	371.0
18	736.0
19	1531.0
20	2694.0
21	4348.0
22	6549.0
23	9763.0
24	13804.0
25	19375.0
26	26308.0
27	31944.0
28	36184.0
29	41218.0
30	49078.0
31	59512.0
32	73449.0
33	94163.0
34	155338.0
35	241018.0
36	147661.0
37	186821.0
38	281323.0
39	530262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.935973421992905	23.837671544101763	28.6101420522969	13.616212981608436
2	32.56173352965568	25.592207360101703	27.898812878812336	13.947246231430283
3	29.65389495430043	25.54870524086319	30.712347202530673	14.085052602305712
4	25.824615942334862	28.961982822622094	29.781322394900915	15.432078840142127
5	23.733484961724592	32.21371657574757	28.755298096285685	15.297500366242156
6	20.74256528421633	41.21488110165641	26.750575434710814	11.291978179416445
7	87.093725713179	4.157580964346637	6.707023655518834	2.041669666955522
8	86.74690059815414	3.4849368946141297	6.571054702921743	3.197107804309987
9	80.88955874648346	5.45017989318144	9.538981821974032	4.1212795383610725
10	40.56373979177582	28.955427708764237	15.86565989387668	14.615172605583268
11	35.749356282853164	24.989136885178738	24.532463953081276	14.729042878886823
12	32.490769456149025	22.546115474289802	26.669778690417367	18.293336379143813
13	23.584803061039533	31.685036711120603	27.166080265382792	17.564079962457075
14	18.9408525124212	33.56486458972188	28.321717042551132	19.17256585530579
15	19.159157667869266	23.687499844812645	39.85573783517365	17.29760465214444
16	21.24224373601762	22.135328339197347	37.022538169881734	19.599889754903298
17	20.397180307842053	22.777729497267458	29.458780997122208	27.36630919776828
18	23.46204365606509	22.84347927565992	32.50090008665662	21.193576981618367
19	26.57085606310787	27.62593143450224	26.270413344622696	19.53279915776719
20	28.36030282639625	25.672457844906997	25.463488760711034	20.50375056798572
21	24.31798261405029	29.499253859199133	26.67082154944021	19.511941977310368
22	23.434184422169196	25.235698554150453	26.646835791914864	24.683281231765484
23	20.868254626445413	29.795376161732534	26.655526283771874	22.680842928050176
24	21.27690638353872	25.692570126061792	33.19186867921905	19.838654811180444
25	20.824156587765277	26.821291208450138	30.483762436714596	21.870789767069986
26	19.320453196735354	29.433951020387894	28.372072235368318	22.873523547508437
27	20.060386503417845	27.980950441849433	28.642967281539654	23.31569577319306
28	18.355212681165717	28.404152565309047	32.53571171403812	20.70492303948711
29	19.947509429183665	25.626472727995054	30.97931911237799	23.446698730443288
30	22.04966491946397	27.033438529668096	30.680167552683002	20.236728998184926
31	25.992565904965748	26.014614924305818	26.257203797000045	21.735615373728397
32	26.112494692592474	26.850590580996624	25.842841145257843	21.194073581153056
33	24.642212450246934	26.804158524503464	26.76870131772687	21.78492770752274
34	19.834880654716827	28.79716143705973	28.727438862389786	22.640519045833653
35	20.061131402719877	27.4902107816725	31.29182919955604	21.156828616051587
36	24.585798743106576	29.677533092151492	26.074256528421635	19.662411636320297
37	22.752998840440085	30.161717638470577	27.555960560064957	19.529322961024388
38	21.738197691308763	31.407089951556717	25.96818286781265	20.886529489321866
39	23.43696537956344	29.02589518273621	25.67444424304575	21.862695194654602
40	24.726783351004002	25.612418961163435	26.31570322218608	23.345094465646486
41	20.115211092047204	25.981591055249183	26.806889821944235	27.096308030759374
42	22.473661602179078	27.036318806969277	25.189415477517695	25.300604113333947
43	21.622092720099122	25.910478001882115	27.419643987793584	25.047785290225182
44	21.56255043589024	28.266296534480144	27.77267659700203	22.398476432627582
45	20.51482473760922	29.69953245153809	26.272797022389188	23.512845788463498
46	23.01013807950062	29.350522298560605	26.268774566158232	21.370565055780546
47	21.746292263724147	26.76750947884362	27.901146896625356	23.585051360806876
48	22.46775206771631	24.760502459409196	29.428041485925128	23.343703986949365
49	22.764917229272555	24.096151601905948	29.57299889010004	23.565932278721455
50	20.866367548213606	27.816029736380138	28.583424997330773	22.73417771807548
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1578.0
1	1949.0
2	2320.0
3	9693.5
4	17067.0
5	12075.5
6	7084.0
7	7216.0
8	7348.0
9	7856.0
10	8364.0
11	8382.5
12	8401.0
13	8205.5
14	8010.0
15	7675.5
16	7341.0
17	7393.5
18	7446.0
19	7293.5
20	7141.0
21	7759.0
22	8377.0
23	9414.5
24	10452.0
25	11743.5
26	13035.0
27	15909.5
28	18784.0
29	21877.0
30	24970.0
31	29331.0
32	33692.0
33	38224.0
34	42756.0
35	50856.0
36	58956.0
37	62991.5
38	67027.0
39	78089.0
40	89151.0
41	110543.5
42	131936.0
43	144196.0
44	156456.0
45	169013.5
46	181571.0
47	186173.5
48	190776.0
49	188747.0
50	186718.0
51	172555.5
52	158393.0
53	141153.5
54	123914.0
55	109586.0
56	95258.0
57	88388.0
58	81518.0
59	75070.5
60	68623.0
61	64073.5
62	59524.0
63	52013.5
64	44503.0
65	36370.0
66	28237.0
67	24335.5
68	20434.0
69	16257.0
70	12080.0
71	10736.5
72	9393.0
73	7681.5
74	5970.0
75	5027.0
76	4084.0
77	3481.0
78	2878.0
79	2156.5
80	1435.0
81	964.5
82	494.0
83	299.0
84	104.0
85	73.0
86	42.0
87	33.0
88	24.0
89	17.5
90	11.0
91	11.0
92	11.0
93	7.5
94	4.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2013695.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.400704550444512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56867962902034	17.59993779288213
2	7.0506485966731365	3.158789922061623
3	2.4515147973463676	1.6474697602919652
4	1.2782925614288096	1.1453861599039086
5	0.947244839449456	1.0609475892720257
6	0.6492391315949936	0.872604838166797
7	0.5369957666064621	0.8420358458813572
8	0.478458615399475	0.857424806654272
9	0.3901978102370365	0.7866635277015241
>10	5.287154363647165	28.794687608692954
>50	2.0135690916681366	32.01758425589154
>100	0.343551761510015	9.819602932917672
>500	0.003117124793061206	0.4925212081573336
>1k	0.0011132588546647164	0.45072548436636184
>5k	2.2265177093294328E-4	0.4536182671585771
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9095	0.4516572767971317	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3303	0.16402682630686374	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04896471412006287	0.0
2	0.0	0.0	0.0	0.19198538010969884	0.0
3	0.0	0.0	0.0	0.3272590933582295	0.0
4	0.0	0.0	0.0	0.549636364990726	0.0
5	0.0	0.0	0.0	0.9238737743302734	0.0
6	0.0	0.0	0.0	1.7042302831362246	0.0
7	0.0	0.0	0.0	2.1790787582032034	0.0
8	0.0	0.0	0.0	3.573530251602154	0.0
9	0.0	0.0	0.0	4.401758955551859	0.0
10	0.0	0.0	0.0	5.535942632821753	0.0
11	0.0	0.0	0.0	6.1124450326390045	0.0
12	0.0	0.0	0.0	6.655923563399621	0.0
13	0.0	0.0	0.0	6.927116569291774	0.0
14	4.96599534686236E-5	0.0	0.0	7.048833115243371	0.0
15	4.96599534686236E-5	0.0	0.0	7.1592271918041215	0.0
16	4.96599534686236E-5	0.0	0.0	7.377433027345253	0.0
17	4.96599534686236E-5	0.0	0.0	7.598469480234097	0.0
18	4.96599534686236E-5	0.0	0.0	7.960142921346082	0.0
19	4.96599534686236E-5	0.0	0.0	8.103958146591216	0.0
20	4.96599534686236E-5	0.0	0.0	8.288593853587559	0.0
21	4.96599534686236E-5	0.0	0.0	8.470647243003533	0.0
22	4.96599534686236E-5	0.0	0.0	8.647486337305303	0.0
23	4.96599534686236E-5	0.0	0.0	8.85427038354865	0.0
24	4.96599534686236E-5	0.0	0.0	9.01814822999511	0.0
25	4.96599534686236E-5	0.0	0.0	9.151137585384083	0.0
26	4.96599534686236E-5	0.0	0.0	9.28507047988896	0.0
27	4.96599534686236E-5	0.0	0.0	9.428637405366752	0.0
28	4.96599534686236E-5	0.0	0.0	9.56718867554421	0.0
29	4.96599534686236E-5	0.0	0.0	9.727093725713178	0.0
30	4.96599534686236E-5	0.0	0.0	9.947534259160399	0.0
31	4.96599534686236E-5	0.0	0.0	10.104062432493501	0.0
32	4.96599534686236E-5	0.0	0.0	10.271962735170916	0.0
33	4.96599534686236E-5	0.0	0.0	10.436088881384718	0.0
34	4.96599534686236E-5	0.0	0.0	10.594454472996159	0.0
35	4.96599534686236E-5	0.0	0.0	10.789022170686225	0.0
36	4.96599534686236E-5	0.0	0.0	10.94078298848634	0.0
37	4.96599534686236E-5	0.0	0.0	11.106150633536856	0.0
38	4.96599534686236E-5	0.0	0.0	11.294461177089877	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CACTACG	25	4.4440374E-5	44.0	1
CCGAATT	25	4.4440374E-5	44.0	30
TCCGTCG	35	1.446806E-7	43.999996	16
CGTTTTT	6420	0.0	41.66978	1
CACGACG	160	0.0	39.875	26
CGGTCTA	175	0.0	38.971428	31
TCGATAG	80	0.0	38.5	1
TCGAAAT	40	4.1265957E-7	38.5	10
GACCGAT	1155	0.0	38.47619	9
TAGCACG	150	0.0	38.13333	1
AGGGCGA	2615	0.0	37.690247	6
AACGCCG	100	0.0	37.399998	13
AGGGCGC	365	0.0	37.369865	6
GGGCGAT	4790	0.0	37.110645	7
ACGGGTC	155	0.0	36.903225	5
GCGCGAC	585	0.0	36.854702	9
CGTAATC	30	1.3014427E-4	36.666668	34
TTACGAG	60	1.9826984E-10	36.666668	1
ACGGGAT	530	0.0	36.5283	5
CGAATAT	230	0.0	36.347828	14
>>END_MODULE