FastQCFastQC Report
Thu 26 May 2016
SRR1546528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546528_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1927060
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89880.4664099716666839No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA38920.20196568866563572No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGC254.443966E-544.029
GTATACG351.4467696E-744.01
CGTTTTT65700.041.756471
TATAACG950.041.684211
CGCGTCC404.1264684E-738.520
GTACGGG5200.038.53
CGCATCG1150.038.2608721
CGGTCTA1100.038.031
CTAACCG357.2927705E-637.7142871
CGTAAGG3850.037.7142872
GGTACCT8300.037.3734938
GGCGATA9550.037.3193748
TCTAGCG651.0913936E-1137.230771
TCGTACA651.0913936E-1137.2307734
AACGGGA7450.037.2080544
CGTTAGG2550.037.098042
TCGATAG950.037.052631
TAACGGG6100.036.7868843
ACGGGAT6600.036.6666685
TAGCGAG1200.036.6666641
Produced by FastQC (version 0.11.5)