FastQCFastQC Report
Thu 26 May 2016
SRR1546527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546527_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1339296
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75030.560219697512723No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21630.16150275965880584No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACGC207.8569475E-444.00000433
CTATGCG502.7284841E-1144.01
CGACGGT351.446333E-744.028
CTTACGT254.4432345E-544.019
CGTTTTT48850.041.7482071
CGTTAGG2450.041.306122
GCGTAAG1050.039.8095251
GTATGCG452.3514076E-839.1111071
GGTTACG404.1252133E-738.5000041
ACGGGAT5400.038.2962955
TAGGGAC9650.038.072545
GCGCGAC3300.038.09
ACGGGTC1450.037.9310345
TGATCGC357.29111E-637.71428743
GGCGATT7000.037.7142878
TCGTTGA1000.037.424
AACGGGA3300.037.3333324
TACGGGA3950.037.3164564
ATCGTTG950.037.05263523
GGCGCGA3400.036.8823558
Produced by FastQC (version 0.11.5)