##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546527_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1339296
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.410662019449024	31.0	31.0	33.0	30.0	34.0
2	31.798929437555252	31.0	31.0	34.0	30.0	34.0
3	31.942115857883543	33.0	31.0	34.0	30.0	34.0
4	35.62138765440948	37.0	35.0	37.0	33.0	37.0
5	35.50433212672927	37.0	35.0	37.0	33.0	37.0
6	35.543708784316536	37.0	35.0	37.0	33.0	37.0
7	36.0101471220701	37.0	35.0	37.0	35.0	37.0
8	35.99521689006762	37.0	35.0	37.0	35.0	37.0
9	37.68849007239624	39.0	37.0	39.0	35.0	39.0
10	37.054029131722935	39.0	37.0	39.0	33.0	39.0
11	36.8104063627458	39.0	37.0	39.0	32.0	39.0
12	36.366800916302296	38.0	35.0	39.0	32.0	39.0
13	36.17889547941605	39.0	35.0	39.0	32.0	39.0
14	37.22906213413614	39.0	35.0	41.0	32.0	41.0
15	37.40789788067761	39.0	35.0	41.0	32.0	41.0
16	37.48432236040427	39.0	35.0	41.0	32.0	41.0
17	37.42946219506368	39.0	35.0	41.0	32.0	41.0
18	37.36595270948319	39.0	35.0	41.0	32.0	41.0
19	37.353297553341456	39.0	35.0	41.0	32.0	41.0
20	37.21049939669797	39.0	35.0	41.0	32.0	41.0
21	37.09128228561871	39.0	35.0	41.0	32.0	41.0
22	37.02303672974458	39.0	35.0	41.0	32.0	41.0
23	36.93220318734619	39.0	35.0	41.0	32.0	41.0
24	36.881340644637184	38.0	35.0	41.0	32.0	41.0
25	36.70313731990538	38.0	35.0	41.0	31.0	41.0
26	36.58889819726185	38.0	35.0	41.0	31.0	41.0
27	36.48608672018732	38.0	35.0	40.0	31.0	41.0
28	36.55598239672186	38.0	35.0	40.0	31.0	41.0
29	36.504208181014505	38.0	35.0	40.0	31.0	41.0
30	36.48491520918453	38.0	35.0	40.0	31.0	41.0
31	36.38546071966167	38.0	35.0	40.0	30.0	41.0
32	36.19379285833752	38.0	35.0	40.0	30.0	41.0
33	36.14299527512962	38.0	35.0	41.0	30.0	41.0
34	36.069055682985685	38.0	35.0	41.0	30.0	41.0
35	35.905373420065466	38.0	35.0	41.0	29.0	41.0
36	35.7457134195876	38.0	35.0	40.0	29.0	41.0
37	35.71558490430793	38.0	35.0	40.0	29.0	41.0
38	35.65141761044608	38.0	35.0	40.0	29.0	41.0
39	35.5608916923518	38.0	35.0	40.0	28.0	41.0
40	35.45984233507753	38.0	35.0	40.0	27.0	41.0
41	35.46611055360428	38.0	35.0	40.0	27.0	41.0
42	35.29339966669056	38.0	34.0	40.0	27.0	41.0
43	35.31545080400449	38.0	34.0	40.0	27.0	41.0
44	34.97267967648675	38.0	34.0	40.0	26.0	41.0
45	34.938949268869614	37.0	34.0	40.0	26.0	41.0
46	34.96066366210307	37.0	34.0	40.0	26.0	41.0
47	34.89615813083889	37.0	34.0	40.0	26.0	41.0
48	34.867623736649705	37.0	34.0	40.0	26.0	41.0
49	34.86084554870619	37.0	34.0	40.0	26.0	41.0
50	34.315167072850215	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	3.0
12	4.0
13	9.0
14	13.0
15	39.0
16	107.0
17	220.0
18	485.0
19	998.0
20	1715.0
21	2980.0
22	4240.0
23	6384.0
24	8988.0
25	12917.0
26	17739.0
27	21736.0
28	24348.0
29	27434.0
30	31764.0
31	38847.0
32	47756.0
33	61458.0
34	99683.0
35	156117.0
36	98751.0
37	125002.0
38	189873.0
39	359683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.83785511193941	23.77331075430674	27.514604687835998	13.874229445917855
2	32.06669772776145	24.898603445392204	29.014198504288817	14.020500322557522
3	29.62100984397773	25.154260148615393	30.92146918978329	14.303260817623586
4	25.603451365493513	28.274705516928293	30.972615463646573	15.149227653931618
5	22.842373903901752	32.796260124722245	29.307113588034312	15.054252383341696
6	20.555799464793445	41.165059852340335	27.368408477289563	10.910732205576661
7	86.30743315891334	4.3812570186127635	7.147038444078083	2.164271378395814
8	86.16108761618044	3.7778056531192505	6.923712159223951	3.1373945714763574
9	80.38372398633312	5.812456693665926	9.901321291185818	3.9024980288151387
10	41.64740281461305	27.43874393711323	16.35934102692758	14.554512221346142
11	33.86801722696103	25.409020858719806	25.575526246625092	15.147435667694072
12	31.862560628867705	22.330164504336604	27.879945882015626	17.927328984780065
13	22.871941676821255	31.659991517931807	28.82379996654959	16.644266838697344
14	18.775759802164718	34.2771874178673	29.440840561011157	17.506212218956826
15	17.439983394260867	25.083028695672947	40.867963467373905	16.60902444269228
16	19.28505722409385	22.497939215826822	39.26368778821112	18.953315771868205
17	19.876114018111007	22.70715360906028	30.30151661768571	27.115215755143
18	22.837819272214656	23.68199412228514	32.93222708049603	20.54795952500418
19	26.213025350631973	26.27372888442883	27.166959357752134	20.34628640718706
20	27.511319379733827	24.703276945499724	26.84059386423912	20.94480981052732
21	23.54535517167228	28.434864286908944	28.206983370367716	19.81279717105106
22	22.890010871383172	25.190099873366307	27.78541860798509	24.13447064726543
23	20.867530404033165	29.483325568059637	27.123951688051033	22.52519233985616
24	20.868949059804553	25.711642534585334	33.30555754665138	20.113850858958738
25	20.07375516689365	26.639294076888152	32.22618450290302	21.060766253315176
26	19.2681080209304	29.94856999498244	28.861133013165123	21.922188970922036
27	20.046278044584618	29.37625439036628	29.2854604209973	21.2920071440518
28	19.15043425799823	27.858442047165077	33.497897402814615	19.49322629202208
29	19.54063926122381	26.18129226100877	32.895566028719564	21.38250244904786
30	20.785397701478985	27.54596444699305	30.243501063245166	21.4251367882828
31	25.141268248393185	26.71119752466968	25.90891035290182	22.238623874035316
32	25.25371538479918	27.399544238166918	26.741661290707953	20.60507908632595
33	23.829982319069124	27.687232695386232	25.90943301555444	22.573351969990206
34	20.041275416338138	28.351088930303682	28.868300958115306	22.739334695242874
35	21.06524621890904	27.18622320980575	28.663641196568946	23.08488937471627
36	24.819009390007885	26.687528373115427	26.75838649559171	21.735075741284973
37	22.265354335412038	28.957601605619672	27.353549924736576	21.423494134231717
38	21.758968891118915	30.45973406924235	25.27268057247987	22.508616467158866
39	21.02903316369197	29.011062528373117	28.018750149332188	21.94115415860273
40	23.242285499247366	26.13962858098583	27.42985867201873	23.18822724774807
41	19.50741284973598	25.404914223592094	29.03510501039352	26.052567916278402
42	22.77741440279072	27.074522734332067	26.356682914008555	23.79137994886866
43	22.169109741237186	27.30367297445822	27.403426875014937	23.123790409289658
44	21.411024896662127	29.2998709769909	27.924820204047503	21.364283922299478
45	20.082267101521996	30.084387618569757	26.432021002078702	23.401324277829545
46	22.153504527751895	28.82469595966836	27.65094497407593	21.37085453850381
47	22.286858170262587	26.95147301268726	28.021886125247892	22.73978269180226
48	22.89740281461305	24.9031580770793	29.04645425656464	23.152984851743007
49	21.59828745848565	24.891958163094642	29.75548347788689	23.754270900532816
50	20.218383389482238	28.49937579146059	28.533348863880725	22.74889195517645
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1482.0
1	1662.5
2	1843.0
3	7201.0
4	12559.0
5	8898.5
6	5238.0
7	5448.5
8	5659.0
9	5956.0
10	6253.0
11	6227.0
12	6201.0
13	6204.0
14	6207.0
15	5897.5
16	5588.0
17	5382.5
18	5177.0
19	5325.5
20	5474.0
21	5961.5
22	6449.0
23	6390.5
24	6332.0
25	7710.5
26	9089.0
27	11193.5
28	13298.0
29	14838.0
30	16378.0
31	19753.0
32	23128.0
33	26547.5
34	29967.0
35	34346.5
36	38726.0
37	43662.5
38	48599.0
39	55832.5
40	63066.0
41	75882.0
42	88698.0
43	100066.5
44	111435.0
45	117266.5
46	123098.0
47	124339.0
48	125580.0
49	123214.5
50	120849.0
51	111715.5
52	102582.0
53	90872.5
54	79163.0
55	72235.0
56	65307.0
57	59781.0
58	54255.0
59	48343.5
60	42432.0
61	38289.5
62	34147.0
63	30709.5
64	27272.0
65	22117.5
66	16963.0
67	13964.5
68	10966.0
69	9424.5
70	7883.0
71	6380.5
72	4878.0
73	3895.0
74	2912.0
75	2302.0
76	1692.0
77	1601.0
78	1510.0
79	1049.5
80	589.0
81	403.0
82	217.0
83	145.0
84	73.0
85	58.5
86	44.0
87	30.5
88	17.0
89	12.5
90	8.0
91	9.5
92	11.0
93	6.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1339296.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.82563942829376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.68791010655193	19.534776836712506
2	6.257447946775578	3.1069029293593524
3	2.245591455053264	1.6724473129922959
4	1.393689533923645	1.3839693537670075
5	0.9373589167226628	1.1635267240726432
6	0.7337897355216968	1.093007963414681
7	0.5837126878466222	1.0143728502740301
8	0.48767127525518283	0.9685400991217091
9	0.43843791414512445	0.9796051411434068
>10	6.639227392657678	41.4825046931646
>50	1.5350083001078592	24.556477908678577
>100	0.05803873971992252	2.054185150556012
>500	0.0012091404107850367	0.17732946738125954
>1k	6.045702053925183E-4	0.24929686442680668
>5k	3.0228510269625917E-4	0.5630567049350784
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7503	0.560219697512723	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2163	0.16150275965880584	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04517298640479774	0.0
2	0.0	0.0	0.0	0.18121460827180846	0.0
3	0.0	0.0	0.0	0.2994856999498244	0.0
4	0.0	0.0	0.0	0.4824922944591786	0.0
5	0.0	0.0	0.0	0.8150550737103672	0.0
6	0.0	0.0	0.0	1.495337849138652	0.0
7	0.0	0.0	0.0	1.8836015339402192	0.0
8	0.0	0.0	0.0	3.0919975867918668	0.0
9	0.0	0.0	0.0	3.766381740854897	0.0
10	0.0	0.0	0.0	4.6913453038014	0.0
11	0.0	0.0	0.0	5.17256827467565	0.0
12	0.0	0.0	0.0	5.637588703318759	0.0
13	0.0	0.0	0.0	5.877042864310802	0.0
14	0.0	0.0	0.0	5.982546054046305	0.0
15	0.0	0.0	0.0	6.081105297111318	0.0
16	0.0	0.0	0.0	6.2819570879028985	0.0
17	0.0	0.0	0.0	6.493560796119753	0.0
18	0.0	0.0	0.0	6.856139344849831	0.0
19	0.0	0.0	0.0	6.984863689580197	0.0
20	0.0	0.0	0.0	7.1545797194944205	0.0
21	0.0	0.0	0.0	7.324594413781568	0.0
22	0.0	0.0	0.0	7.504241034095525	0.0
23	0.0	0.0	0.0	7.701359520225552	0.0
24	0.0	0.0	0.0	7.845689078441211	0.0
25	0.0	0.0	0.0	7.9788933887654405	0.0
26	0.0	0.0	0.0	8.104556423673333	0.0
27	0.0	0.0	0.0	8.24029938116742	0.0
28	0.0	0.0	0.0	8.384703605476309	0.0
29	7.466609323107066E-5	0.0	0.0	8.530003822903973	0.0
30	7.466609323107066E-5	0.0	0.0	8.729660956203857	0.0
31	7.466609323107066E-5	0.0	0.0	8.893329032566363	0.0
32	1.493321864621413E-4	0.0	0.0	9.041914558096193	0.0
33	1.493321864621413E-4	0.0	0.0	9.197966692949132	0.0
34	1.493321864621413E-4	0.0	0.0	9.355661481853152	0.0
35	1.493321864621413E-4	0.0	0.0	9.533441449836332	0.0
36	1.493321864621413E-4	0.0	0.0	9.689717582968964	0.0
37	1.493321864621413E-4	0.0	0.0	9.85696963180656	0.0
38	1.493321864621413E-4	0.0	0.0	10.027506988746326	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTACGC	20	7.8569475E-4	44.000004	33
CTATGCG	50	2.7284841E-11	44.0	1
CGACGGT	35	1.446333E-7	44.0	28
CTTACGT	25	4.4432345E-5	44.0	19
CGTTTTT	4885	0.0	41.748207	1
CGTTAGG	245	0.0	41.30612	2
GCGTAAG	105	0.0	39.809525	1
GTATGCG	45	2.3514076E-8	39.111107	1
GGTTACG	40	4.1252133E-7	38.500004	1
ACGGGAT	540	0.0	38.296295	5
TAGGGAC	965	0.0	38.07254	5
GCGCGAC	330	0.0	38.0	9
ACGGGTC	145	0.0	37.931034	5
TGATCGC	35	7.29111E-6	37.714287	43
GGCGATT	700	0.0	37.714287	8
TCGTTGA	100	0.0	37.4	24
AACGGGA	330	0.0	37.333332	4
TACGGGA	395	0.0	37.316456	4
ATCGTTG	95	0.0	37.052635	23
GGCGCGA	340	0.0	36.882355	8
>>END_MODULE