FastQCFastQC Report
Thu 26 May 2016
SRR1546526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546526_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1300318
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86750.6671444984995978No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA18840.14488763517847172No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATGCG302.527975E-644.01
TCTAGCG454.802132E-1044.01
CCGAACC207.856863E-444.025
TCGACGG254.4431632E-544.02
CGTTTCG207.856863E-444.042
CGTTTTT56650.041.9417461
AATGCGG1150.040.1739162
TACGGGA5350.039.887854
CGTATGG2000.039.6000022
CGACAAT501.3478711E-939.60000220
ACGGGAT5550.038.450455
AAGGGCG6700.038.0895545
TTACGGG4000.037.953
TAACGGG2500.037.843
CGGGTAT700.037.7142876
CGAAAGG1700.037.529412
GCGATCG651.0913936E-1137.2307669
GCGATAC651.0913936E-1137.2307669
TAAGGGA16750.037.170154
TCCGCTA301.3011895E-436.66666441
Produced by FastQC (version 0.11.5)