##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546526_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1300318
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.422518953056098	31.0	31.0	33.0	30.0	34.0
2	31.817726894498115	31.0	31.0	34.0	30.0	34.0
3	31.9474859226743	33.0	31.0	34.0	30.0	34.0
4	35.63099564875669	37.0	35.0	37.0	33.0	37.0
5	35.521673928992755	37.0	35.0	37.0	33.0	37.0
6	35.55777279096344	37.0	35.0	37.0	33.0	37.0
7	36.0282546269451	37.0	35.0	37.0	35.0	37.0
8	36.02099640241848	37.0	35.0	37.0	35.0	37.0
9	37.7057012207783	39.0	37.0	39.0	35.0	39.0
10	37.06059517748735	39.0	37.0	39.0	33.0	39.0
11	36.859369784929534	39.0	37.0	39.0	32.0	39.0
12	36.5566053842214	39.0	35.0	39.0	32.0	39.0
13	36.46025126161447	39.0	35.0	39.0	32.0	39.0
14	37.55960388151206	40.0	36.0	41.0	32.0	41.0
15	37.685311593010326	40.0	36.0	41.0	33.0	41.0
16	37.72252325969494	40.0	36.0	41.0	33.0	41.0
17	37.661449737679554	40.0	36.0	41.0	33.0	41.0
18	37.58599358003196	39.0	36.0	41.0	32.0	41.0
19	37.56956452190926	39.0	36.0	41.0	33.0	41.0
20	37.40738957701116	39.0	35.0	41.0	32.0	41.0
21	37.307633209722546	39.0	35.0	41.0	32.0	41.0
22	37.2349409913575	39.0	35.0	41.0	32.0	41.0
23	37.13016123748191	39.0	35.0	41.0	32.0	41.0
24	37.08728710976853	39.0	35.0	41.0	32.0	41.0
25	36.93382849426064	39.0	35.0	41.0	31.0	41.0
26	36.847029726574576	39.0	35.0	41.0	31.0	41.0
27	36.729033974766175	39.0	35.0	41.0	31.0	41.0
28	36.78868399883721	39.0	35.0	41.0	31.0	41.0
29	36.72904551040592	39.0	35.0	41.0	31.0	41.0
30	36.711332920101086	39.0	35.0	41.0	31.0	41.0
31	36.603966875794995	39.0	35.0	41.0	31.0	41.0
32	36.41476085080726	39.0	35.0	41.0	30.0	41.0
33	36.33927931475224	39.0	35.0	41.0	30.0	41.0
34	36.2345810793975	39.0	35.0	41.0	30.0	41.0
35	36.053058559521595	39.0	35.0	41.0	29.0	41.0
36	35.923596381808146	39.0	35.0	41.0	29.0	41.0
37	35.862523628835405	38.0	35.0	41.0	28.0	41.0
38	35.83237715697237	38.0	35.0	41.0	28.0	41.0
39	35.725461002616285	38.0	35.0	41.0	27.0	41.0
40	35.655423519477544	38.0	35.0	40.0	27.0	41.0
41	35.66213956893621	38.0	35.0	40.0	27.0	41.0
42	35.495069667573624	38.0	35.0	40.0	27.0	41.0
43	35.55036537216281	38.0	35.0	40.0	27.0	41.0
44	35.22374142325185	38.0	34.0	40.0	26.0	41.0
45	35.167102201153874	38.0	34.0	40.0	26.0	41.0
46	35.192424468476176	38.0	34.0	40.0	26.0	41.0
47	35.115358704563036	38.0	34.0	40.0	26.0	41.0
48	35.09809062090965	38.0	34.0	40.0	26.0	41.0
49	35.09767149266564	38.0	34.0	40.0	26.0	41.0
50	34.55751823784644	37.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	3.0
13	5.0
14	6.0
15	38.0
16	72.0
17	196.0
18	432.0
19	952.0
20	1609.0
21	2624.0
22	3964.0
23	5702.0
24	8583.0
25	12230.0
26	17196.0
27	20841.0
28	23153.0
29	25538.0
30	29551.0
31	35896.0
32	44539.0
33	56388.0
34	87539.0
35	129176.0
36	96516.0
37	124974.0
38	193263.0
39	379329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.52047883671533	23.911996911524717	27.107599833271557	14.459924418488402
2	33.4556623841245	25.01341979423495	28.241091794468737	13.289826027171813
3	29.37412233007618	25.2518230156008	31.160839117815797	14.213215536507223
4	25.353874975198377	28.280312969596665	31.18467943995238	15.181132615252576
5	22.55978922079061	33.01923068049508	29.831625802303748	14.58935429641057
6	20.39378059828442	40.98905037075546	27.900790422035225	10.716378608924893
7	86.30965656093356	3.9129659052631744	7.867690826397851	1.9096867074054193
8	85.9968100110896	3.4939914697789307	7.611830336886823	2.8973681822446506
9	79.51478023068204	5.956850555018081	10.470900195183026	4.057469019116862
10	37.41077182658396	31.850901087272497	17.27062149412682	13.46770559201672
11	29.855619932970246	26.36678104894341	28.022991299051466	15.754607719034883
12	27.883948388009706	24.062114036720246	30.236603661565866	17.817333913704186
13	22.789271547421478	29.21600716132515	30.94719906976601	17.04752222148736
14	19.07956361443893	31.863974812315142	31.24458786235367	17.81187371089226
15	18.237769530222607	25.81899196965665	39.497030726330024	16.446207773790718
16	19.733865100690753	24.19592745774495	37.5221292022413	18.548078239322997
17	20.31180065184055	23.984363824848998	31.047174614209755	24.656660909100697
18	22.620620494371376	24.484318451332673	32.94424902216227	19.950812032133676
19	25.78592313572526	26.262652674192005	28.81625879208009	19.135165398002645
20	26.378239784421964	25.711095285922365	27.918786020035096	19.99187890962057
21	23.56600462348441	27.325315807364042	29.58491692032257	19.523762648828978
22	22.529104419072873	25.42139691983038	29.13287365090693	22.916625010189815
23	20.351560156823176	28.789419203610194	28.81295190868695	22.04606873087968
24	20.02410179663744	25.988335161091364	33.66553412319141	20.32202891907979
25	19.750937847511146	27.726140836318503	31.416391990266995	21.106529325903356
26	19.231910963318207	29.583609547818302	30.04326633946465	21.141213149398837
27	20.418313058805616	28.197179459178447	30.25406092971104	21.1304465523049
28	19.26021173282228	27.2567941072876	33.46358352341504	20.019410636475076
29	19.768625828451196	25.828451194246334	32.484515326250964	21.91840765105151
30	20.856052134939297	27.17512177790356	30.71894721137445	21.249878875782695
31	23.50940308447626	26.871426835589446	27.578715360396455	22.040454719537834
32	23.90069198457608	27.24818082961245	27.788048769608665	21.063078416202803
33	22.839643840968133	27.90748109308646	27.96339049371	21.289484572235406
34	20.69985957281219	27.867644683838876	28.84463646584912	22.58785927749981
35	20.51736575206988	27.79735418566843	30.144549256412663	21.54073080584903
36	24.11540869233526	27.302321432141984	27.618628673908997	20.963641201613758
37	21.299866648004564	29.68466175197144	28.852711413669578	20.162760186354415
38	21.363081953798993	30.178540941523536	27.29986049566337	21.158516609014104
39	21.155132821356005	29.382197277896637	27.319855604552117	22.142814296195237
40	22.443509972176038	27.178044139971913	28.4242008493307	21.954245038521346
41	19.307277142975796	26.76268420494064	28.989601005292553	24.94043764679102
42	22.056220093853966	27.94831725777848	27.15812593534812	22.83733671301943
43	21.602715643404153	27.471203197986956	28.477034079355974	22.44904707925292
44	21.314709171141214	28.75235134790105	28.70705473584154	21.225884745116193
45	19.79977205575867	29.669742324569835	28.3955924627668	22.134893156904695
46	21.83865792829139	29.13233532105223	28.066211495957145	20.962795254699234
47	21.09476297336498	27.27309781145843	29.387272959383782	22.244866255792815
48	22.03314881436695	25.921120833519183	29.731035023740347	22.31469532837352
49	21.354391771858882	25.27881641260061	29.94129128413204	23.42550053140847
50	20.01625756161185	28.228787112075658	29.640826320946108	22.11412900536638
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1049.0
1	1267.5
2	1486.0
3	8008.5
4	14531.0
5	10240.0
6	5949.0
7	6019.0
8	6089.0
9	6345.0
10	6601.0
11	6482.5
12	6364.0
13	6209.0
14	6054.0
15	5796.0
16	5538.0
17	5457.0
18	5376.0
19	5454.0
20	5532.0
21	6100.5
22	6669.0
23	7154.5
24	7640.0
25	9194.5
26	10749.0
27	13864.0
28	16979.0
29	19623.5
30	22268.0
31	24912.0
32	27556.0
33	32706.5
34	37857.0
35	41519.5
36	45182.0
37	49990.5
38	54799.0
39	60812.0
40	66825.0
41	77870.5
42	88916.0
43	96468.5
44	104021.0
45	109197.5
46	114374.0
47	116400.0
48	118426.0
49	115550.5
50	112675.0
51	101920.5
52	91166.0
53	80802.0
54	70438.0
55	62310.0
56	54182.0
57	50993.0
58	47804.0
59	44226.5
60	40649.0
61	36160.5
62	31672.0
63	26925.0
64	22178.0
65	18494.5
66	14811.0
67	12479.0
68	10147.0
69	8704.0
70	7261.0
71	5919.5
72	4578.0
73	3547.5
74	2517.0
75	2116.0
76	1715.0
77	1229.0
78	743.0
79	603.0
80	463.0
81	358.5
82	254.0
83	193.0
84	132.0
85	107.0
86	82.0
87	46.5
88	11.0
89	7.5
90	4.0
91	4.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1300318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.131644687845146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.15826198513565	20.934305489123695
2	6.046322021501361	3.28093321511337
3	2.477154051373892	2.0162779067679755
4	1.522631762162032	1.6524601584563112
5	1.0878504144310985	1.4757585458934828
6	0.9023401912195818	1.468918407343916
7	0.7379276479566428	1.4014833524787835
8	0.6515969936638081	1.4143118489403619
9	0.5849179273151356	1.4282806837918582
>10	8.219502843356148	53.298170144739075
>50	0.5850686871227374	9.515205199445084
>100	0.025004750312944853	1.141441651482356
>500	8.524346693536825E-4	0.15842648879425836
>1k	2.841448897845608E-4	0.14524355469021058
>5k	2.841448897845608E-4	0.6687833529392658
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8675	0.6671444984995978	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1884	0.14488763517847172	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04521970779455487	0.0
2	0.0	0.0	0.0	0.18449333163118561	0.0
3	0.0	0.0	0.0	0.3130772626388314	0.0
4	0.0	0.0	0.0	0.48495829481711394	0.0
5	0.0	0.0	0.0	0.8352572216950007	0.0
6	0.0	0.0	0.0	1.475946653049485	0.0
7	0.0	0.0	0.0	1.8496244764742162	0.0
8	0.0	0.0	0.0	2.9415112303298114	0.0
9	0.0	0.0	0.0	3.561744127205807	0.0
10	0.0	0.0	0.0	4.3960784977213265	0.0
11	0.0	0.0	0.0	4.832279488555876	0.0
12	0.0	0.0	0.0	5.269018809245123	0.0
13	0.0	0.0	0.0	5.489964762465797	0.0
14	0.0	0.0	0.0	5.601783563712877	0.0
15	0.0	0.0	0.0	5.704450757430106	0.0
16	0.0	0.0	0.0	5.90986204912952	0.0
17	0.0	0.0	0.0	6.118887841281902	0.0
18	0.0	0.0	0.0	6.4523447341342655	0.0
19	0.0	0.0	0.0	6.593079539005074	0.0
20	0.0	0.0	0.0	6.768267454576496	0.0
21	0.0	0.0	0.0	6.937841358806077	0.0
22	0.0	0.0	0.0	7.118797094249253	0.0
23	0.0	0.0	0.0	7.327284556546937	0.0
24	0.0	0.0	0.0	7.478170724392033	0.0
25	0.0	0.0	0.0	7.606908463929592	0.0
26	0.0	0.0	0.0	7.742644491578214	0.0
27	0.0	0.0	0.0	7.896760638551493	0.0
28	0.0	0.0	0.0	8.031343102225764	0.0
29	0.0	0.0	0.0	8.183921163899907	0.0
30	0.0	0.0	0.0	8.39617693518047	0.0
31	0.0	0.0	0.0	8.554599720991327	0.0
32	0.0	0.0	0.0	8.731710243186667	0.0
33	0.0	0.0	0.0	8.898823210937632	0.0
34	0.0	0.0	0.0	9.066935934133035	0.0
35	0.0	0.0	0.0	9.249275946345433	0.0
36	7.690426495672597E-5	0.0	0.0	9.415542967181874	0.0
37	7.690426495672597E-5	0.0	0.0	9.589961840103728	0.0
38	7.690426495672597E-5	0.0	0.0	9.772071139521255	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATGCG	30	2.527975E-6	44.0	1
TCTAGCG	45	4.802132E-10	44.0	1
CCGAACC	20	7.856863E-4	44.0	25
TCGACGG	25	4.4431632E-5	44.0	2
CGTTTCG	20	7.856863E-4	44.0	42
CGTTTTT	5665	0.0	41.941746	1
AATGCGG	115	0.0	40.173916	2
TACGGGA	535	0.0	39.88785	4
CGTATGG	200	0.0	39.600002	2
CGACAAT	50	1.3478711E-9	39.600002	20
ACGGGAT	555	0.0	38.45045	5
AAGGGCG	670	0.0	38.089554	5
TTACGGG	400	0.0	37.95	3
TAACGGG	250	0.0	37.84	3
CGGGTAT	70	0.0	37.714287	6
CGAAAGG	170	0.0	37.52941	2
GCGATCG	65	1.0913936E-11	37.230766	9
GCGATAC	65	1.0913936E-11	37.230766	9
TAAGGGA	1675	0.0	37.17015	4
TCCGCTA	30	1.3011895E-4	36.666664	41
>>END_MODULE