FastQCFastQC Report
Thu 26 May 2016
SRR1546525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546525_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2083929
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150860.7239210165029615No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA21710.10417821336523461No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT96750.041.7715761
CGACGGT1000.039.628
GCGTAAG1000.039.61
TACGGGA7550.039.337754
GTACGAG1850.039.243241
TTACGGG5500.038.43
ACGGGAC5000.037.845
CGTAAGG2500.037.842
TACGGGT1050.037.7142874
GGGCGAT43650.036.238267
TAGGGAT29200.036.1643835
CTATACG553.426976E-936.01
AGGGCGA22850.035.719916
CGGGATC5550.035.6756746
TAGGGCG9750.035.651285
GTCGACA250.00235318335.233
CTACGGG3700.035.081083
TAAGGGA25650.035.079924
AAGGGAT28700.035.031365
AAGGGAC20700.034.85995
Produced by FastQC (version 0.11.5)