##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546525_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2083929
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.433918334069922	31.0	31.0	33.0	30.0	34.0
2	31.830280686146217	31.0	31.0	34.0	30.0	34.0
3	31.96073618630961	33.0	31.0	34.0	30.0	34.0
4	35.64122434113638	37.0	35.0	37.0	33.0	37.0
5	35.534111766763644	37.0	35.0	37.0	33.0	37.0
6	35.56546024360715	37.0	35.0	37.0	33.0	37.0
7	36.02668948894132	37.0	35.0	37.0	35.0	37.0
8	36.01852366371407	37.0	35.0	37.0	35.0	37.0
9	37.70389634195791	39.0	37.0	39.0	35.0	39.0
10	37.108262325635856	39.0	37.0	39.0	33.0	39.0
11	36.86772246079401	39.0	37.0	39.0	32.0	39.0
12	36.58918753949871	39.0	35.0	39.0	32.0	39.0
13	36.50475376080471	39.0	35.0	39.0	32.0	39.0
14	37.61489522915608	40.0	36.0	41.0	32.0	41.0
15	37.734960740025215	40.0	36.0	41.0	33.0	41.0
16	37.76888032173841	40.0	36.0	41.0	33.0	41.0
17	37.69700263300717	40.0	36.0	41.0	33.0	41.0
18	37.621493342623474	39.0	36.0	41.0	33.0	41.0
19	37.590986545127016	39.0	36.0	41.0	33.0	41.0
20	37.44180152010937	39.0	35.0	41.0	32.0	41.0
21	37.34019057271145	39.0	35.0	41.0	32.0	41.0
22	37.266221641908146	39.0	35.0	41.0	32.0	41.0
23	37.1662345502174	39.0	35.0	41.0	32.0	41.0
24	37.112140096903495	39.0	35.0	41.0	32.0	41.0
25	36.95585070316695	39.0	35.0	41.0	31.0	41.0
26	36.84609888340726	39.0	35.0	41.0	31.0	41.0
27	36.75619898758547	39.0	35.0	41.0	31.0	41.0
28	36.79864813052652	39.0	35.0	41.0	31.0	41.0
29	36.72921918165158	39.0	35.0	41.0	31.0	41.0
30	36.696403284372934	39.0	35.0	41.0	31.0	41.0
31	36.58537694902274	39.0	35.0	41.0	31.0	41.0
32	36.39633547975963	39.0	35.0	41.0	30.0	41.0
33	36.30513419603067	39.0	35.0	41.0	30.0	41.0
34	36.18913216333186	39.0	35.0	41.0	30.0	41.0
35	36.008642328985296	39.0	35.0	41.0	29.0	41.0
36	35.8918283684329	39.0	35.0	41.0	29.0	41.0
37	35.850314957947226	38.0	35.0	41.0	28.0	41.0
38	35.81473601067982	38.0	35.0	41.0	28.0	41.0
39	35.70591272543354	38.0	35.0	41.0	28.0	41.0
40	35.607363782547296	38.0	35.0	40.0	27.0	41.0
41	35.6038823779505	38.0	35.0	40.0	27.0	41.0
42	35.41664999143445	38.0	35.0	40.0	27.0	41.0
43	35.45449389110666	38.0	35.0	40.0	27.0	41.0
44	35.20647488470097	38.0	34.0	40.0	26.0	41.0
45	35.12669817445796	38.0	34.0	40.0	26.0	41.0
46	35.120726761804264	38.0	34.0	40.0	26.0	41.0
47	35.039096341574016	38.0	34.0	40.0	26.0	41.0
48	34.98600336191876	38.0	34.0	40.0	26.0	41.0
49	34.97472898548847	37.0	34.0	40.0	26.0	41.0
50	34.4415932596552	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	3.0
13	10.0
14	17.0
15	52.0
16	99.0
17	297.0
18	746.0
19	1478.0
20	2521.0
21	4222.0
22	6287.0
23	9264.0
24	13284.0
25	19517.0
26	27776.0
27	34293.0
28	37531.0
29	40926.0
30	47751.0
31	56608.0
32	70711.0
33	90606.0
34	139287.0
35	205525.0
36	159714.0
37	205867.0
38	311936.0
39	597595.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.70633068593028	23.7305109723028	27.418880393717828	15.144277948049092
2	33.340483289018	25.397026482188213	27.603147708007324	13.659342520786458
3	29.708593718883897	25.223700039684655	31.404572804543722	13.663133436887726
4	25.419340102277953	28.386043862338877	30.68223533527294	15.512380700110223
5	23.015467417555975	32.280802273014096	29.79468110477852	14.909049204651406
6	20.488030062444544	40.66961974232328	27.856131374917283	10.986218820314896
7	85.63962591815748	3.9118415262708086	8.250329065913474	2.198203489658237
8	84.9675780700782	3.5119238707268816	8.213715534454389	3.3067825247405267
9	78.12497450728888	6.277277200902717	11.359456104310656	4.23829218749775
10	38.4329312562952	31.851373055415994	16.512174838970044	13.203520849318762
11	29.18962210324824	25.77856539258295	29.314818307149622	15.716994197019188
12	27.675990880687394	22.79895332326581	30.86160804902662	18.66344774702017
13	22.293417865963765	28.19496249632305	31.68303718600778	17.828582451705408
14	18.978669618782597	31.18230035668202	30.95599706132023	18.883032963215157
15	18.087804334984543	24.85175838524249	39.50278536360884	17.55765191616413
16	20.510919517891445	23.70099941024862	36.90624776563885	18.881833306221086
17	20.59086465997642	23.58276121691286	31.021882223434677	24.804491899676044
18	22.29946413721389	24.045588885225936	33.08836337514378	20.566583602416397
19	25.405711998825296	26.48482745813317	28.393961598499757	19.715498944541775
20	26.766698865460388	25.17264263801694	27.762846047058225	20.297812449464452
21	24.0977499713282	27.35385898463911	29.117834628722957	19.430556415309734
22	23.095604504759997	24.94518767194084	28.6128270204983	23.346380802800862
23	20.57694863884518	28.806643604460614	28.21458888474607	22.40181887194813
24	20.432893826996985	25.20220218635088	33.21571896163449	21.149185025017648
25	20.843560409207797	26.677012508583548	31.083496606650225	21.395930475558426
26	19.85182796534815	28.706448252315695	29.94986873353171	21.491855048804446
27	20.7080471551574	28.17927098284059	29.36395625762682	21.748725604375196
28	19.833689151597774	27.679062002592218	32.57150315581769	19.915745689992317
29	20.734583567866274	25.397266413587026	31.903198237559916	21.96495178098678
30	21.517239790799014	26.901780242992924	30.655890867683112	20.92508909852495
31	23.958205869777714	26.43213852295352	27.4552539937781	22.15440161349067
32	24.197129556717144	27.399541922973388	27.30481700672144	21.098511513588033
33	23.11067219660555	26.550856579086908	27.947401279026302	22.391069945281245
34	20.289606795624994	27.64820682470468	29.065337638662353	22.996848741007973
35	20.152414021782892	26.83445549248559	29.33924332354893	23.673887162182588
36	22.98341258267436	26.722503501798766	27.172758764813963	23.121325150712906
37	20.675176553519815	28.81537710737746	28.518438008204694	21.99100833089803
38	20.908677790846042	28.62923832817721	26.77768772352609	23.684396157450664
39	20.74638819268795	27.819661802297485	28.577269187194	22.856680817820568
40	22.471159046205507	25.580861919959847	29.1312228007768	22.816756233057845
41	19.604938555968076	25.94685327571141	29.54097764367212	24.907230524648394
42	21.968022902891605	26.753886528763697	27.91395484203157	23.364135726313133
43	21.889517349199515	25.655480584991142	29.24106339515406	23.21393867065529
44	21.644547391009965	27.77124364601673	28.78322629993632	21.800982663036987
45	19.823564046567803	28.56863165683668	28.572422572937946	23.035381723657572
46	22.124554147478154	28.012710605783592	27.863569248280534	21.99916599845772
47	21.522902171811037	26.700093909149498	28.940765256397892	22.836238662641577
48	21.831933813484046	24.805019748753438	29.643428350965888	23.719618086796622
49	21.801462525834616	24.817976044289416	29.89761167486992	23.48294975500605
50	20.331882708096103	27.272810158119594	29.299318738786205	23.095988394998102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1664.0
1	2065.0
2	2466.0
3	14016.0
4	25566.0
5	17910.5
6	10255.0
7	10383.0
8	10511.0
9	10835.5
10	11160.0
11	11116.0
12	11072.0
13	10727.0
14	10382.0
15	10177.0
16	9972.0
17	9788.0
18	9604.0
19	9251.0
20	8898.0
21	9947.0
22	10996.0
23	11458.0
24	11920.0
25	14427.5
26	16935.0
27	20283.5
28	23632.0
29	27610.0
30	31588.0
31	36707.5
32	41827.0
33	47690.0
34	53553.0
35	59329.0
36	65105.0
37	72411.5
38	79718.0
39	89002.5
40	98287.0
41	114494.5
42	130702.0
43	144365.0
44	158028.0
45	165376.0
46	172724.0
47	182211.0
48	191698.0
49	186138.0
50	180578.0
51	163478.5
52	146379.0
53	131486.5
54	116594.0
55	104094.0
56	91594.0
57	88200.5
58	84807.0
59	78630.0
60	72453.0
61	65633.5
62	58814.0
63	51089.0
64	43364.0
65	36374.5
66	29385.0
67	25389.0
68	21393.0
69	18383.0
70	15373.0
71	12779.5
72	10186.0
73	8321.0
74	6456.0
75	5069.0
76	3682.0
77	3053.0
78	2424.0
79	1877.5
80	1331.0
81	894.5
82	458.0
83	360.0
84	262.0
85	164.0
86	66.0
87	50.0
88	34.0
89	29.5
90	25.0
91	14.0
92	3.0
93	2.0
94	1.0
95	2.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2083929.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.093936025156427
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76798524926075	19.515048466502577
2	6.693022104033207	3.359085369871343
3	2.44922000512498	1.8438171036041864
4	1.3704611305267127	1.375610557376035
5	0.9433621759655848	1.1836335046116373
6	0.7446511789343664	1.121173742714178
7	0.6068383025176366	1.0659573078694466
8	0.5461090719279721	1.0963220890974466
9	0.4591687983746595	1.037011720604549
>10	7.091337546765916	44.93472072533602
>50	1.2732398532926297	20.022337253710603
>100	0.050566946617724734	1.9151059607850285
>500	0.0028840261841462968	0.45724357016108913
>1k	9.613420613820989E-4	0.34506763889333225
>5k	0.0	0.0
>10k+	1.922684122764198E-4	0.7278649888625294
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15086	0.7239210165029615	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	2171	0.10417821336523461	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06924420169785055	0.0
2	4.798627976289019E-5	0.0	0.0	0.2432424521180904	0.0
3	4.798627976289019E-5	0.0	0.0	0.411674294085835	0.0
4	4.798627976289019E-5	0.0	0.0	0.6371138364118931	0.0
5	4.798627976289019E-5	0.0	0.0	1.07023799755174	0.0
6	4.798627976289019E-5	0.0	0.0	1.87515985429446	0.0
7	4.798627976289019E-5	0.0	0.0	2.349936106268496	0.0
8	4.798627976289019E-5	0.0	0.0	3.6791560557005543	0.0
9	4.798627976289019E-5	0.0	0.0	4.412914259554908	0.0
10	4.798627976289019E-5	0.0	0.0	5.423985174159005	0.0
11	4.798627976289019E-5	0.0	0.0	5.960951644705745	0.0
12	9.597255952578039E-5	0.0	0.0	6.475940399121083	0.0
13	9.597255952578039E-5	0.0	0.0	6.749750111448135	0.0
14	9.597255952578039E-5	0.0	0.0	6.879888902165093	0.0
15	9.597255952578039E-5	0.0	0.0	6.998750917137772	0.0
16	9.597255952578039E-5	0.0	0.0	7.218624051011335	0.0
17	9.597255952578039E-5	0.0	0.0	7.448526317355341	0.0
18	9.597255952578039E-5	0.0	0.0	7.815477398702163	0.0
19	9.597255952578039E-5	0.0	0.0	7.965770426919535	0.0
20	9.597255952578039E-5	0.0	0.0	8.151765247280498	0.0
21	9.597255952578039E-5	0.0	0.0	8.334832904575924	0.0
22	9.597255952578039E-5	0.0	0.0	8.5316726241633	0.0
23	9.597255952578039E-5	0.0	0.0	8.741612598125943	0.0
24	9.597255952578039E-5	0.0	0.0	8.910956179409183	0.0
25	9.597255952578039E-5	0.0	0.0	9.056882456168132	0.0
26	9.597255952578039E-5	0.0	0.0	9.201992966171112	0.0
27	9.597255952578039E-5	0.0	0.0	9.356412814448094	0.0
28	9.597255952578039E-5	0.0	0.0	9.513040031594167	0.0
29	9.597255952578039E-5	0.0	0.0	9.670435029216446	0.0
30	9.597255952578039E-5	0.0	0.0	9.895778598982979	0.0
31	9.597255952578039E-5	0.0	0.0	10.075967079492631	0.0
32	9.597255952578039E-5	0.0	0.0	10.256299518841573	0.0
33	9.597255952578039E-5	0.0	0.0	10.437783628904823	0.0
34	9.597255952578039E-5	0.0	0.0	10.614181193313208	0.0
35	9.597255952578039E-5	0.0	0.0	10.806942079120738	0.0
36	9.597255952578039E-5	0.0	0.0	10.981516164898132	0.0
37	9.597255952578039E-5	0.0	0.0	11.162712357282805	0.0
38	9.597255952578039E-5	0.0	0.0	11.353985668417685	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	9675	0.0	41.771576	1
CGACGGT	100	0.0	39.6	28
GCGTAAG	100	0.0	39.6	1
TACGGGA	755	0.0	39.33775	4
GTACGAG	185	0.0	39.24324	1
TTACGGG	550	0.0	38.4	3
ACGGGAC	500	0.0	37.84	5
CGTAAGG	250	0.0	37.84	2
TACGGGT	105	0.0	37.714287	4
GGGCGAT	4365	0.0	36.23826	7
TAGGGAT	2920	0.0	36.164383	5
CTATACG	55	3.426976E-9	36.0	1
AGGGCGA	2285	0.0	35.71991	6
CGGGATC	555	0.0	35.675674	6
TAGGGCG	975	0.0	35.65128	5
GTCGACA	25	0.002353183	35.2	33
CTACGGG	370	0.0	35.08108	3
TAAGGGA	2565	0.0	35.07992	4
AAGGGAT	2870	0.0	35.03136	5
AAGGGAC	2070	0.0	34.8599	5
>>END_MODULE