FastQCFastQC Report
Thu 26 May 2016
SRR1546524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546524_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2124749
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147530.6943408374353864No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA31210.1468879382929466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT254.44412E-544.042
CGTACGT254.44412E-544.031
TATCGCG551.8189894E-1244.01
CGTTTTT85550.041.6341321
ACGGGAT5900.038.779665
GCGATAA1950.038.3589749
TACGGGA6400.038.1562544
GGCGATA8400.037.7142878
CGCATCG950.037.0526321
TCGTAAG1250.036.961
AGGGCGA24200.036.8181846
TCATACG301.3014674E-436.6666641
GTACGTA301.3014674E-436.66666415
CTAATCC9200.036.58695211
ATGGGAC13300.036.5563935
CGTTAGG3200.036.43752
TATACTA8400.035.88095544
GGGCGAT46200.035.523817
AAGGGCG11400.035.5087745
GTTTTTT106250.035.3656462
Produced by FastQC (version 0.11.5)