##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546524_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2124749
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.383519182736407	31.0	31.0	33.0	30.0	34.0
2	31.774459947975032	31.0	31.0	34.0	30.0	34.0
3	31.914131504474174	31.0	31.0	34.0	30.0	34.0
4	35.601371738497114	37.0	35.0	37.0	33.0	37.0
5	35.49091116174193	37.0	35.0	37.0	33.0	37.0
6	35.52658455186942	37.0	35.0	37.0	33.0	37.0
7	35.98883868165134	37.0	35.0	37.0	35.0	37.0
8	35.9668285524549	37.0	35.0	37.0	35.0	37.0
9	37.64732587237363	39.0	37.0	39.0	35.0	39.0
10	37.035555258527005	39.0	37.0	39.0	33.0	39.0
11	36.80597049345593	39.0	37.0	39.0	32.0	39.0
12	36.41364885922996	39.0	35.0	39.0	32.0	39.0
13	36.295663158330704	39.0	35.0	39.0	32.0	39.0
14	37.3549558088979	39.0	35.0	41.0	32.0	41.0
15	37.49707989037764	39.0	35.0	41.0	32.0	41.0
16	37.546032025429824	39.0	35.0	41.0	32.0	41.0
17	37.46740909161505	39.0	35.0	41.0	32.0	41.0
18	37.39996065417609	39.0	36.0	41.0	32.0	41.0
19	37.383204792660216	39.0	36.0	41.0	32.0	41.0
20	37.22718824670584	39.0	35.0	41.0	32.0	41.0
21	37.12422220224601	39.0	35.0	41.0	32.0	41.0
22	37.057938608277965	39.0	35.0	41.0	32.0	41.0
23	36.962842669887124	39.0	35.0	41.0	31.0	41.0
24	36.878890636023364	39.0	35.0	41.0	31.0	41.0
25	36.69319670229284	38.0	35.0	41.0	31.0	41.0
26	36.58775977774316	38.0	35.0	41.0	31.0	41.0
27	36.512614901807225	38.0	35.0	40.0	31.0	41.0
28	36.547865183134576	38.0	35.0	40.0	31.0	41.0
29	36.474627826627994	38.0	35.0	40.0	30.0	41.0
30	36.42995855039819	38.0	35.0	40.0	30.0	41.0
31	36.33925042440307	38.0	35.0	40.0	30.0	41.0
32	36.16232882095721	38.0	35.0	40.0	30.0	41.0
33	36.090586699887844	38.0	35.0	41.0	30.0	41.0
34	35.9895837108289	38.0	35.0	41.0	29.0	41.0
35	35.82144196796892	38.0	35.0	41.0	29.0	41.0
36	35.70490302619274	38.0	35.0	40.0	28.0	41.0
37	35.67901149735804	38.0	35.0	40.0	28.0	41.0
38	35.61631609192427	38.0	35.0	40.0	28.0	41.0
39	35.512710913147856	38.0	35.0	40.0	27.0	41.0
40	35.38593029106026	38.0	34.0	40.0	27.0	41.0
41	35.37080897555429	38.0	34.0	40.0	27.0	41.0
42	35.170852180657576	38.0	34.0	40.0	26.0	41.0
43	35.18979700661114	38.0	34.0	40.0	27.0	41.0
44	34.84197474619355	37.0	34.0	40.0	25.0	41.0
45	34.774833168529554	37.0	34.0	40.0	25.0	41.0
46	34.80057079683294	37.0	34.0	40.0	25.0	41.0
47	34.71984643833225	37.0	34.0	40.0	25.0	41.0
48	34.685337421031846	37.0	34.0	40.0	25.0	41.0
49	34.66818574805777	36.0	34.0	40.0	26.0	41.0
50	34.11539010019536	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	1.0
11	6.0
12	3.0
13	10.0
14	27.0
15	65.0
16	154.0
17	367.0
18	842.0
19	1657.0
20	2889.0
21	4617.0
22	7097.0
23	10315.0
24	14637.0
25	20677.0
26	28754.0
27	35616.0
28	39939.0
29	44512.0
30	51856.0
31	62336.0
32	77038.0
33	98090.0
34	156031.0
35	226283.0
36	167407.0
37	213454.0
38	312459.0
39	547606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.751893517775514	23.07962022808341	26.763631845455627	14.40485440868545
2	33.360222783961774	24.983562764354755	27.757396285396535	13.898818166286937
3	30.334453622522002	25.321437967496397	30.213027515250033	14.131080894731566
4	25.931815946260006	27.989000112483875	30.247572772125086	15.831611169131035
5	23.21605987342505	32.409616382923346	29.111250317096278	15.263073426555323
6	20.662864178310002	40.79851314202289	27.268350285139565	11.270272394527542
7	85.50487610536585	4.269963181533442	7.851986281673741	2.373174431426959
8	84.85096357263846	3.780587730597826	7.821394432942433	3.5470542638212796
9	78.68600008753975	5.729382623547535	11.027184858070296	4.55743243084242
10	41.32229265668557	28.269739155071967	16.38814749412754	14.019820694114928
11	33.012134609782144	25.156524370643307	25.63728703955149	16.194053980023053
12	29.67992925281998	22.214623939109984	29.317439377545302	18.788007430524733
13	23.610600593293608	28.019262510536542	30.198155170328356	18.171981725841498
14	19.92978935394251	30.168739931163636	30.923181985260378	18.978288729633476
15	19.03909591203479	23.135862165366355	39.31509086485039	18.50995105774847
16	21.272394998185668	22.54369810269354	36.172978549466315	20.010928349654478
17	21.707599344675536	22.34640421056793	29.976623121131013	25.969373323625522
18	24.304706108815676	22.572642698031625	32.04519686795946	21.077454325193234
19	26.848865442459317	25.747605952514864	26.883740150013015	20.519788455012804
20	28.038370649897942	24.056535619030768	26.84547680690755	21.05961692416375
21	25.11915525080845	27.3181444019976	27.330004626428817	20.232695720765133
22	24.092681064916373	23.841733776554314	27.609002286858352	24.45658287167096
23	22.63166143389172	27.244771029425124	26.621403281046373	23.502164255636785
24	22.503175669220223	24.343298902599788	31.835007334984038	21.318518093195948
25	22.5312260412877	25.122967465804198	29.697437203170825	22.648369289737282
26	20.587325844135	27.55765504537242	28.57735196016094	23.277667150331638
27	21.514776568902963	26.573350546346887	28.323910259517714	23.58796262523244
28	21.032366646601552	26.440346600939684	31.801544558910255	20.72574219354851
29	21.916753461232364	24.3336036397711	30.975517578782245	22.774125320214296
30	22.602434452257654	25.959678060796826	29.624628603190306	21.81325888375521
31	25.97958629466351	25.006247796798586	26.545582560575394	22.46858334796251
32	26.14930045854828	25.17064368544237	26.633451763008242	22.0466040930011
33	24.950241181428957	26.36631432700992	26.385751917049966	22.297692574511156
34	21.404410591556932	25.922356005344632	29.700919967487927	22.972313435610513
35	21.66142918528259	26.24215848554347	30.520404998425697	21.576007330748244
36	24.96308975789611	25.602271138849815	27.267408997486292	22.16723010576779
37	22.659523548428545	27.33348739074592	28.4903769809987	21.516612079826842
38	22.58370282795756	27.854066527387474	26.489481816440435	23.072748828214532
39	22.981302732699252	26.111507759269447	27.24632415405302	23.66086535397828
40	24.076114402218803	24.634721560052505	27.68246978819616	23.606694249532534
41	21.526072020742216	24.40933023147675	28.011402758631725	26.053194989149308
42	23.87750270737861	25.807942491089538	26.145252921639216	24.16930187989264
43	23.204999743499112	25.02827392788513	27.833358199015507	23.93336812960025
44	22.381043596208304	27.05232476871386	28.11179108685308	22.454840548224755
45	21.066535388415293	27.641429646513544	27.82803992377453	23.46399504129664
46	23.258559010970238	27.096235837738952	27.20919035613148	22.436014795159334
47	22.30931747702905	25.74633521418295	28.425851712366967	23.518495596421037
48	23.159959129290094	24.31995496879867	28.560079331723422	23.96000657018782
49	23.01773056488084	23.867195607575294	29.254278976010813	23.86079485153305
50	21.11670602033464	26.451312602100295	29.4046026142382	23.02737876332687
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1397.0
1	1846.0
2	2295.0
3	13246.5
4	24198.0
5	16550.5
6	8903.0
7	8993.5
8	9084.0
9	9373.0
10	9662.0
11	9755.5
12	9849.0
13	9415.0
14	8981.0
15	8965.5
16	8950.0
17	8612.0
18	8274.0
19	8105.5
20	7937.0
21	8410.5
22	8884.0
23	10024.0
24	11164.0
25	12392.0
26	13620.0
27	15384.5
28	17149.0
29	20370.5
30	23592.0
31	28412.5
32	33233.0
33	39428.0
34	45623.0
35	49626.5
36	53630.0
37	60407.5
38	67185.0
39	74917.0
40	82649.0
41	100088.5
42	117528.0
43	131396.5
44	145265.0
45	160685.5
46	176106.0
47	182358.0
48	188610.0
49	187361.0
50	186112.0
51	171602.5
52	157093.0
53	142341.5
54	127590.0
55	116676.5
56	105763.0
57	102802.0
58	99841.0
59	95281.5
60	90722.0
61	84856.0
62	78990.0
63	70325.0
64	61660.0
65	52818.0
66	43976.0
67	37077.5
68	30179.0
69	26281.0
70	22383.0
71	18896.5
72	15410.0
73	12777.0
74	10144.0
75	7721.5
76	5299.0
77	4036.0
78	2773.0
79	2184.5
80	1596.0
81	1198.5
82	801.0
83	563.5
84	326.0
85	284.0
86	242.0
87	144.5
88	47.0
89	32.5
90	18.0
91	13.0
92	8.0
93	7.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2124749.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.30744795600501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37510574448989	19.050988039305768
2	6.592447485210523	3.204911482989018
3	2.377670308089245	1.7338529181125315
4	1.3508206578882131	1.3134001135805675
5	0.945687067687311	1.149361959023815
6	0.6736143811776176	0.9824307907734877
7	0.5718606168142089	0.9730330526910214
8	0.47892905206296393	0.9313234406111447
9	0.4305627651219444	0.9419293804495746
>10	6.48965497601964	39.585421669782114
>50	1.6196148890230553	25.532773481735592
>100	0.09109967644352868	3.2512208614754416
>500	0.0019549199812280544	0.3208965883346283
>1k	7.819679924912218E-4	0.32740670718578246
>5k	0.0	0.0
>10k+	1.9549199812280544E-4	0.7010495139494701
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14753	0.6943408374353864	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	3121	0.1468879382929466	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04866457167411303	0.0
2	0.0	0.0	0.0	0.1747971172124331	0.0
3	0.0	0.0	0.0	0.3006943408374354	0.0
4	0.0	0.0	0.0	0.48556323593986866	0.0
5	0.0	0.0	0.0	0.8192967734071177	0.0
6	0.0	0.0	0.0	1.5476651595082525	0.0
7	0.0	0.0	0.0	1.9771747156958304	0.0
8	0.0	0.0	0.0	3.3215217420975374	0.0
9	0.0	0.0	0.0	4.088341728834794	0.0
10	0.0	0.0	0.0	5.1147217859615415	0.0
11	0.0	0.0	0.0	5.618216551696224	0.0
12	0.0	0.0	0.0	6.118510939409784	0.0
13	0.0	0.0	0.0	6.376235498875396	0.0
14	0.0	0.0	0.0	6.496155545902128	0.0
15	0.0	0.0	0.0	6.604874269854934	0.0
16	0.0	0.0	0.0	6.796520436060918	0.0
17	9.412876532710452E-5	0.0	0.0	6.990425692634753	0.0
18	9.412876532710452E-5	0.0	0.0	7.318793890478358	0.0
19	9.412876532710452E-5	0.0	0.0	7.460222360382332	0.0
20	9.412876532710452E-5	0.0	0.0	7.632148550252289	0.0
21	9.412876532710452E-5	0.0	0.0	7.804968963392852	0.0
22	9.412876532710452E-5	0.0	0.0	7.981507462763837	0.0
23	9.412876532710452E-5	0.0	0.0	8.178001260384168	0.0
24	9.412876532710452E-5	0.0	0.0	8.334113817679171	0.0
25	9.412876532710452E-5	0.0	0.0	8.467070698703706	0.0
26	9.412876532710452E-5	0.0	0.0	8.600121708493567	0.0
27	9.412876532710452E-5	0.0	0.0	8.746256616663898	0.0
28	9.412876532710452E-5	0.0	0.0	8.8872615071239	0.0
29	9.412876532710452E-5	0.0	0.0	9.03499660430479	0.0
30	9.412876532710452E-5	0.0	0.0	9.259776095905917	0.0
31	9.412876532710452E-5	0.0	0.0	9.424454370845686	0.0
32	9.412876532710452E-5	0.0	0.0	9.624619190313773	0.0
33	9.412876532710452E-5	0.0	0.0	9.802710814312656	0.0
34	1.8825753065420905E-4	0.0	0.0	9.97355452338135	0.0
35	1.8825753065420905E-4	0.0	0.0	10.160305993790326	0.0
36	2.353219133177613E-4	0.0	0.0	10.323760594780842	0.0
37	2.353219133177613E-4	0.0	0.0	10.502793506432996	0.0
38	2.353219133177613E-4	0.0	0.0	10.706958798427484	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACCGT	25	4.44412E-5	44.0	42
CGTACGT	25	4.44412E-5	44.0	31
TATCGCG	55	1.8189894E-12	44.0	1
CGTTTTT	8555	0.0	41.634132	1
ACGGGAT	590	0.0	38.77966	5
GCGATAA	195	0.0	38.358974	9
TACGGGA	640	0.0	38.156254	4
GGCGATA	840	0.0	37.714287	8
CGCATCG	95	0.0	37.05263	21
TCGTAAG	125	0.0	36.96	1
AGGGCGA	2420	0.0	36.818184	6
TCATACG	30	1.3014674E-4	36.666664	1
GTACGTA	30	1.3014674E-4	36.666664	15
CTAATCC	920	0.0	36.586952	11
ATGGGAC	1330	0.0	36.556393	5
CGTTAGG	320	0.0	36.4375	2
TATACTA	840	0.0	35.880955	44
GGGCGAT	4620	0.0	35.52381	7
AAGGGCG	1140	0.0	35.508774	5
GTTTTTT	10625	0.0	35.365646	2
>>END_MODULE